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  1. 1
    Bacterial Strains and Plasmids Used in This Study.

    Bacterial Strains and Plasmids Used in This Study. von Jianhua Liao (1393228)

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  2. 2
    Membrane integrity in the <i>yqhG</i> mutant.

    Membrane integrity in the <i>yqhG</i> mutant. von Jianhua Liao (1393228)

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  3. 3
    Kidney bacterial burden in mice infected with MRSA wild-type, Δ<i>yqhG</i>, and complemented strains.

    Kidney bacterial burden in mice infected with MRSA wild-type, Δ<i>yqhG</i>, and complemented strains. von Jianhua Liao (1393228)

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  4. 4
    Crystal violet quantification of biofilm formation in MRSA wild-type, ΔyqhG, and complemented strains.

    Crystal violet quantification of biofilm formation in MRSA wild-type, ΔyqhG, and complemented strains. von Jianhua Liao (1393228)

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  5. 5
    Primers Used in This Study.

    Primers Used in This Study. von Jianhua Liao (1393228)

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  6. 6
    Survival data of BALB/c mice infected with MRSA wild-type, Δ<i>yqhG</i>, and complemented strains.

    Survival data of BALB/c mice infected with MRSA wild-type, Δ<i>yqhG</i>, and complemented strains. von Jianhua Liao (1393228)

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  7. 7
    Oxidative stress resistance of the <i>yqhG</i> mutant.

    Oxidative stress resistance of the <i>yqhG</i> mutant. von Jianhua Liao (1393228)

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  8. 8
    YqhG is required for systemic virulence but not for <i>in vitro</i> growth.

    YqhG is required for systemic virulence but not for <i>in vitro</i> growth. von Jianhua Liao (1393228)

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  9. 9
    Optical density measurements of MRSA wild-type, Δ<i>yqhG</i>, and complemented strains during <i>in vitro</i> growth.

    Optical density measurements of MRSA wild-type, Δ<i>yqhG</i>, and complemented strains during <i>in vitro</i> growth. von Jianhua Liao (1393228)

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  10. 10
    Colony diameter measurements from soft agar motility assays.

    Colony diameter measurements from soft agar motility assays. von Jianhua Liao (1393228)

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  11. 11
    Amount of PSMs (μg/1mL culture) in MRSA wild-type, ΔyqhG, and complemented strains.

    Amount of PSMs (μg/1mL culture) in MRSA wild-type, ΔyqhG, and complemented strains. von Jianhua Liao (1393228)

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  12. 12
    Spleen bacterial burden in mice infected with MRSA wild-type, ΔyqhG, and complemented strains.

    Spleen bacterial burden in mice infected with MRSA wild-type, ΔyqhG, and complemented strains. von Jianhua Liao (1393228)

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  13. 13
    Heart bacterial burden in mice infected with MRSA wild-type, Δ<i>yqhG</i>, and complemented strains.

    Heart bacterial burden in mice infected with MRSA wild-type, Δ<i>yqhG</i>, and complemented strains. von Jianhua Liao (1393228)

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  14. 14
    Quantification of cell-associated PSMs in wild-type, Δ<i>yqhG</i>, and complemented strains. Cell-associated PSMs were extracted from bacterial pellets using 6 M guanidine hydrochloride and quantified by reversed-phase HPLC. The Δ<i>yqhG</i> mutant exhibited a significant reduction in surface-bound PSMs compared with the wild-type strain, whereas complementation restored PSM levels to near wild-type values. Data represent mean ± s.e.m. from three independent experiments. Statistical analysis was performed using one-way ANOVA; *<i>P</i> < 0.05, ns, not significant.

    Quantification of cell-associated PSMs in wild-type, Δ<i>yqhG</i>, and complemented strains. Cell-associated PSMs were extracted from bacterial pellets using 6 M guanidine hydrochl... von Jianhua Liao (1393228)

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  15. 15
    SYTOX Green fluorescence measurements for membrane permeability assessment.

    SYTOX Green fluorescence measurements for membrane permeability assessment. von Jianhua Liao (1393228)

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  16. 16
    Biofilm formation in MRSA strains.

    Biofilm formation in MRSA strains. von Jianhua Liao (1393228)

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  17. 17
    Liver bacterial burden in mice infected with MRSA wild-type, ΔyqhG, and complemented strains.

    Liver bacterial burden in mice infected with MRSA wild-type, ΔyqhG, and complemented strains. von Jianhua Liao (1393228)

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  18. 18
    Surface motility of the <i>yqhG</i> mutant.

    Surface motility of the <i>yqhG</i> mutant. von Jianhua Liao (1393228)

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  19. 19
    Viable CFU counts following hydrogen peroxide exposure.

    Viable CFU counts following hydrogen peroxide exposure. von Jianhua Liao (1393228)

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