Application of STUPPIT to identify novel intermediary proteins between SMAD4 and β-catenin, central hubs of BMP and Wnt pathway. nork Lin Xie (136792)

Beste egile batzuk: “...Lijuan Gao (5093777)...”

Validation of the intermediary proteins between β-catenin and SMAD4. nork Lin Xie (136792)

Beste egile batzuk: “...Lijuan Gao (5093777)...”

Immunofluorescence analysis of the indicated proteins expressed in HeLa cells. nork Lin Xie (136792)

Beste egile batzuk: “...Lijuan Gao (5093777)...”

Proximity labeling of the intermediary protein LATS1 between MOB1A and YAP1 by STUPPIT. nork Lin Xie (136792)

Beste egile batzuk: “...Lijuan Gao (5093777)...”

Proximity labeling of the intermediary protein α-catenin between actin and β-catenin by STUPPIT. nork Lin Xie (136792)

Beste egile batzuk: “...Lijuan Gao (5093777)...”

Validation of the YAP1 and actin related plasmids expression in the approach II of STUPPIT. nork Lin Xie (136792)

Beste egile batzuk: “...Lijuan Gao (5093777)...”

Validation of the interaction of RNF20 with β-catenin and SMAD4 using PUP-IT system. nork Lin Xie (136792)

Beste egile batzuk: “...Lijuan Gao (5093777)...”

Design of STUPPIT for labeling and capturing intermediary proteins between two non-interacting proteins. nork Lin Xie (136792)

Beste egile batzuk: “...Lijuan Gao (5093777)...”

Proximity labeling of the intermediary protein AMOT between β-actin and YAP1 by STUPPIT. nork Lin Xie (136792)

Beste egile batzuk: “...Lijuan Gao (5093777)...”

Immunoblotting confirmation that β-actin-PafA-Myc ligates 3 × Flag-TbN-PupE substrate to HA-AMOT. nork Lin Xie (136792)

Beste egile batzuk: “...Lijuan Gao (5093777)...”

Validating the intermediary protein labeling efficiency of STUPPIT by mass spectrometry analysis. nork Lin Xie (136792)

Beste egile batzuk: “...Lijuan Gao (5093777)...”

Analysis of TRIM33 interactions with β-catenin and SMAD proteins. nork Lin Xie (136792)

Beste egile batzuk: “...Lijuan Gao (5093777)...”