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1
Application of STUPPIT to identify novel intermediary proteins between SMAD4 and β-catenin, central hubs of BMP and Wnt pathway.
Izdano 2025Daljnji autori: “…Lijuan Gao (5093777)…”
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2
Validation of the intermediary proteins between β-catenin and SMAD4.
Izdano 2025Daljnji autori: “…Lijuan Gao (5093777)…”
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3
Immunofluorescence analysis of the indicated proteins expressed in HeLa cells.
Izdano 2025Daljnji autori: “…Lijuan Gao (5093777)…”
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4
Proximity labeling of the intermediary protein LATS1 between MOB1A and YAP1 by STUPPIT.
Izdano 2025Daljnji autori: “…Lijuan Gao (5093777)…”
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5
Proximity labeling of the intermediary protein α-catenin between actin and β-catenin by STUPPIT.
Izdano 2025Daljnji autori: “…Lijuan Gao (5093777)…”
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6
Validation of the YAP1 and actin related plasmids expression in the approach II of STUPPIT.
Izdano 2025Daljnji autori: “…Lijuan Gao (5093777)…”
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7
Validation of the interaction of RNF20 with β-catenin and SMAD4 using PUP-IT system.
Izdano 2025Daljnji autori: “…Lijuan Gao (5093777)…”
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8
Design of STUPPIT for labeling and capturing intermediary proteins between two non-interacting proteins.
Izdano 2025Daljnji autori: “…Lijuan Gao (5093777)…”
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9
Proximity labeling of the intermediary protein AMOT between β-actin and YAP1 by STUPPIT.
Izdano 2025Daljnji autori: “…Lijuan Gao (5093777)…”
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10
Immunoblotting confirmation that β-actin-PafA-Myc ligates 3 × Flag-TbN-PupE substrate to HA-AMOT.
Izdano 2025Daljnji autori: “…Lijuan Gao (5093777)…”
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11
Validating the intermediary protein labeling efficiency of STUPPIT by mass spectrometry analysis.
Izdano 2025Daljnji autori: “…Lijuan Gao (5093777)…”
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12
Analysis of TRIM33 interactions with β-catenin and SMAD proteins.
Izdano 2025Daljnji autori: “…Lijuan Gao (5093777)…”