Microsatellite DNA Identification and Genotyping of Candida albicans from Lebanese Clinical Isolates
The present study involves collecting 125 isolates labeled as C. albicans from five different Lebanese hospitals and utilizing the microsatellite genotyping test to determine the following: first, the accuracy of hospital identification by comparing microsatellite results to hospital results. Second...
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2008
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| Online Access: | http://hdl.handle.net/10725/3367 http://dx.doi.org/10.1007/s11046-008-9089-0 http://link.springer.com/article/10.1007/s11046-008-9089-0 |
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| author | Khalaf, Roy A. |
| author2 | Barada, Ghyda Basma, Raida |
| author2_role | author author |
| author_facet | Khalaf, Roy A. Barada, Ghyda Basma, Raida |
| author_role | author |
| dc.creator.none.fl_str_mv | Khalaf, Roy A. Barada, Ghyda Basma, Raida |
| dc.date.none.fl_str_mv | 2008 2016-03-21T08:00:14Z 2016-03-21T08:00:14Z 2016-03-21 |
| dc.identifier.none.fl_str_mv | 0301-486X http://hdl.handle.net/10725/3367 http://dx.doi.org/10.1007/s11046-008-9089-0 Barada, G., Basma, R., & Khalaf, R. A. (2008). Microsatellite DNA identification and genotyping of Candida albicans from Lebanese clinical isolates. Mycopathologia, 165(3), 115-125. http://link.springer.com/article/10.1007/s11046-008-9089-0 |
| dc.language.none.fl_str_mv | en |
| dc.relation.none.fl_str_mv | Mycopathologia |
| dc.rights.*.fl_str_mv | info:eu-repo/semantics/openAccess |
| dc.title.none.fl_str_mv | Microsatellite DNA Identification and Genotyping of Candida albicans from Lebanese Clinical Isolates |
| dc.type.none.fl_str_mv | Article info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article |
| description | The present study involves collecting 125 isolates labeled as C. albicans from five different Lebanese hospitals and utilizing the microsatellite genotyping test to determine the following: first, the accuracy of hospital identification by comparing microsatellite results to hospital results. Second, the frequency and genotypes of infectious strains present relative to tissue and hospital location- a possible indicator of nosocomial infection, and third, a possible relationship between lack of microsatellite heterozygosity to azole resistance. Our results showed that the error in hospital identification varied from 2 to 33%, averaging at 7%, with the highest identification error in stool. Misidentified isolates were mainly Candida tropicalis followed by C. glabrata and C. parapsilosis. Strains with similar genotypes were also found to occur within certain hospitals suggesting the possibility of nosocomial infection. Finally, a relationship between lack of heterozygosity and azole resistance was observed since nine out of 10 homozygous isolates sharing a common allele with a heterozygote strain were sensitive to all drugs tested, whereas the homozygous genotype was resistant to at least one drug. |
| eu_rights_str_mv | openAccess |
| format | article |
| id | LAURepo_0d41e919c9d15664fa40636fc7d8d10c |
| identifier_str_mv | 0301-486X Barada, G., Basma, R., & Khalaf, R. A. (2008). Microsatellite DNA identification and genotyping of Candida albicans from Lebanese clinical isolates. Mycopathologia, 165(3), 115-125. |
| language_invalid_str_mv | en |
| network_acronym_str | LAURepo |
| network_name_str | Lebanese American University repository |
| oai_identifier_str | oai:laur.lau.edu.lb:10725/3367 |
| publishDate | 2008 |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| spelling | Microsatellite DNA Identification and Genotyping of Candida albicans from Lebanese Clinical IsolatesKhalaf, Roy A.Barada, GhydaBasma, RaidaThe present study involves collecting 125 isolates labeled as C. albicans from five different Lebanese hospitals and utilizing the microsatellite genotyping test to determine the following: first, the accuracy of hospital identification by comparing microsatellite results to hospital results. Second, the frequency and genotypes of infectious strains present relative to tissue and hospital location- a possible indicator of nosocomial infection, and third, a possible relationship between lack of microsatellite heterozygosity to azole resistance. Our results showed that the error in hospital identification varied from 2 to 33%, averaging at 7%, with the highest identification error in stool. Misidentified isolates were mainly Candida tropicalis followed by C. glabrata and C. parapsilosis. Strains with similar genotypes were also found to occur within certain hospitals suggesting the possibility of nosocomial infection. Finally, a relationship between lack of heterozygosity and azole resistance was observed since nine out of 10 homozygous isolates sharing a common allele with a heterozygote strain were sensitive to all drugs tested, whereas the homozygous genotype was resistant to at least one drug.PublishedN/A2016-03-21T08:00:14Z2016-03-21T08:00:14Z20082016-03-21Articleinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article0301-486Xhttp://hdl.handle.net/10725/3367http://dx.doi.org/10.1007/s11046-008-9089-0Barada, G., Basma, R., & Khalaf, R. A. (2008). Microsatellite DNA identification and genotyping of Candida albicans from Lebanese clinical isolates. Mycopathologia, 165(3), 115-125.http://link.springer.com/article/10.1007/s11046-008-9089-0enMycopathologiainfo:eu-repo/semantics/openAccessoai:laur.lau.edu.lb:10725/33672016-08-17T08:35:29Z |
| spellingShingle | Microsatellite DNA Identification and Genotyping of Candida albicans from Lebanese Clinical Isolates Khalaf, Roy A. |
| status_str | publishedVersion |
| title | Microsatellite DNA Identification and Genotyping of Candida albicans from Lebanese Clinical Isolates |
| title_full | Microsatellite DNA Identification and Genotyping of Candida albicans from Lebanese Clinical Isolates |
| title_fullStr | Microsatellite DNA Identification and Genotyping of Candida albicans from Lebanese Clinical Isolates |
| title_full_unstemmed | Microsatellite DNA Identification and Genotyping of Candida albicans from Lebanese Clinical Isolates |
| title_short | Microsatellite DNA Identification and Genotyping of Candida albicans from Lebanese Clinical Isolates |
| title_sort | Microsatellite DNA Identification and Genotyping of Candida albicans from Lebanese Clinical Isolates |
| url | http://hdl.handle.net/10725/3367 http://dx.doi.org/10.1007/s11046-008-9089-0 http://link.springer.com/article/10.1007/s11046-008-9089-0 |