Gender specific differences in levels of DNA methylation at selected loci from human total blood
Abnormal patterns of DNA methylation are observed in many diseases such as tumors and imprinting disorders. Little is known about inter-individual and gender specific variations. Here, we report on accurate and sensitive quantitative measurements of methylation in DNA from total blood in 96 healthy...
محفوظ في:
| المؤلف الرئيسي: | |
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| مؤلفون آخرون: | , , , |
| التنسيق: | article |
| منشور في: |
2007
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| الوصول للمادة أونلاين: | http://hdl.handle.net/10725/6180 http://dx.doi.org/10.1007/s00439-007-0430-3 http://libraries.lau.edu.lb/research/laur/terms-of-use/articles.php https://link.springer.com/article/10.1007/s00439-007-0430-3 |
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| _version_ | 1864513478641844224 |
|---|---|
| author | El Maarri, Osman |
| author2 | Becker, Tim Junen, Judith Manzoor, Syed Saadi Diaz-Lacava, Amalia |
| author2_role | author author author author |
| author_facet | El Maarri, Osman Becker, Tim Junen, Judith Manzoor, Syed Saadi Diaz-Lacava, Amalia |
| author_role | author |
| dc.creator.none.fl_str_mv | El Maarri, Osman Becker, Tim Junen, Judith Manzoor, Syed Saadi Diaz-Lacava, Amalia |
| dc.date.none.fl_str_mv | 2007 2017-09-13T09:23:19Z 2017-09-13T09:23:19Z 2017-09-13 |
| dc.identifier.none.fl_str_mv | 1432-1203 http://hdl.handle.net/10725/6180 http://dx.doi.org/10.1007/s00439-007-0430-3 El-Maarri, O., Becker, T., Junen, J., Manzoor, S. S., Diaz-Lacava, A., Schwaab, R., ... & Oldenburg, J. (2007). Gender specific differences in levels of DNA methylation at selected loci from human total blood: a tendency toward higher methylation levels in males. Human genetics, 122(5), 505-514. http://libraries.lau.edu.lb/research/laur/terms-of-use/articles.php https://link.springer.com/article/10.1007/s00439-007-0430-3 |
| dc.language.none.fl_str_mv | en |
| dc.relation.none.fl_str_mv | Human Genetics |
| dc.rights.*.fl_str_mv | info:eu-repo/semantics/openAccess |
| dc.title.none.fl_str_mv | Gender specific differences in levels of DNA methylation at selected loci from human total blood a tendency toward higher methylation levels in males |
| dc.type.none.fl_str_mv | Article info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article |
| description | Abnormal patterns of DNA methylation are observed in many diseases such as tumors and imprinting disorders. Little is known about inter-individual and gender specific variations. Here, we report on accurate and sensitive quantitative measurements of methylation in DNA from total blood in 96 healthy human males and 96 healthy human females. Global methylation was estimated by studying two repetitive DNA elements, namely Line-1 and Alu repeats, while single loci were investigated for three differentially methylated regions (DMRs) at PEG3, NESP55 and H19 imprinted genes and two additional loci at Xq28 (F8 gene) and at 19q13.4 (locus between PEG3 and ubiquitin specific protease 29). We observed inter-individual correlations in the degree of methylation between Alu and Line-1 repeats. Moreover, all studied CpGs showed slightly higher methylation in males (P < 0.0003–0.0381), with the exception of DMRs at imprinted genes (P = 0.0342–0.9616) which were almost equally methylated in both sexes with only a small tendency towards higher methylation in males. This observed difference could be due to the process of X chromosome inactivation or merely to the presence of an additional X chromosome in female cells or could be a result of downstream effects of sex determination. |
| eu_rights_str_mv | openAccess |
| format | article |
| id | LAURepo_0f70a4230e7fbea3afa13deefcbc0ce4 |
| identifier_str_mv | 1432-1203 El-Maarri, O., Becker, T., Junen, J., Manzoor, S. S., Diaz-Lacava, A., Schwaab, R., ... & Oldenburg, J. (2007). Gender specific differences in levels of DNA methylation at selected loci from human total blood: a tendency toward higher methylation levels in males. Human genetics, 122(5), 505-514. |
| language_invalid_str_mv | en |
| network_acronym_str | LAURepo |
| network_name_str | Lebanese American University repository |
| oai_identifier_str | oai:laur.lau.edu.lb:10725/6180 |
| publishDate | 2007 |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| spelling | Gender specific differences in levels of DNA methylation at selected loci from human total blooda tendency toward higher methylation levels in malesEl Maarri, OsmanBecker, TimJunen, JudithManzoor, Syed SaadiDiaz-Lacava, AmaliaAbnormal patterns of DNA methylation are observed in many diseases such as tumors and imprinting disorders. Little is known about inter-individual and gender specific variations. Here, we report on accurate and sensitive quantitative measurements of methylation in DNA from total blood in 96 healthy human males and 96 healthy human females. Global methylation was estimated by studying two repetitive DNA elements, namely Line-1 and Alu repeats, while single loci were investigated for three differentially methylated regions (DMRs) at PEG3, NESP55 and H19 imprinted genes and two additional loci at Xq28 (F8 gene) and at 19q13.4 (locus between PEG3 and ubiquitin specific protease 29). We observed inter-individual correlations in the degree of methylation between Alu and Line-1 repeats. Moreover, all studied CpGs showed slightly higher methylation in males (P < 0.0003–0.0381), with the exception of DMRs at imprinted genes (P = 0.0342–0.9616) which were almost equally methylated in both sexes with only a small tendency towards higher methylation in males. This observed difference could be due to the process of X chromosome inactivation or merely to the presence of an additional X chromosome in female cells or could be a result of downstream effects of sex determination.PublishedN/A2017-09-13T09:23:19Z2017-09-13T09:23:19Z20072017-09-13Articleinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1432-1203http://hdl.handle.net/10725/6180http://dx.doi.org/10.1007/s00439-007-0430-3El-Maarri, O., Becker, T., Junen, J., Manzoor, S. S., Diaz-Lacava, A., Schwaab, R., ... & Oldenburg, J. (2007). Gender specific differences in levels of DNA methylation at selected loci from human total blood: a tendency toward higher methylation levels in males. Human genetics, 122(5), 505-514.http://libraries.lau.edu.lb/research/laur/terms-of-use/articles.phphttps://link.springer.com/article/10.1007/s00439-007-0430-3enHuman Geneticsinfo:eu-repo/semantics/openAccessoai:laur.lau.edu.lb:10725/61802021-03-19T10:00:49Z |
| spellingShingle | Gender specific differences in levels of DNA methylation at selected loci from human total blood El Maarri, Osman |
| status_str | publishedVersion |
| title | Gender specific differences in levels of DNA methylation at selected loci from human total blood |
| title_full | Gender specific differences in levels of DNA methylation at selected loci from human total blood |
| title_fullStr | Gender specific differences in levels of DNA methylation at selected loci from human total blood |
| title_full_unstemmed | Gender specific differences in levels of DNA methylation at selected loci from human total blood |
| title_short | Gender specific differences in levels of DNA methylation at selected loci from human total blood |
| title_sort | Gender specific differences in levels of DNA methylation at selected loci from human total blood |
| url | http://hdl.handle.net/10725/6180 http://dx.doi.org/10.1007/s00439-007-0430-3 http://libraries.lau.edu.lb/research/laur/terms-of-use/articles.php https://link.springer.com/article/10.1007/s00439-007-0430-3 |