Identification, Typing, Antifungal Resistance Profile, and Biofilm Formation of Candida albicans Isolates from Lebanese Hospital Patients
As leading opportunistic fungal pathogens identification and subtyping of Candida species are crucial in recognizing outbreaks of infection, recognizing particularly virulent strains, and detecting the emergence of drug resistant strains. In this study our objective was to compare identification of...
محفوظ في:
| المؤلف الرئيسي: | |
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| مؤلفون آخرون: | , , |
| التنسيق: | article |
| منشور في: |
2014
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| الوصول للمادة أونلاين: | http://hdl.handle.net/10725/3365 http://dx.doi.org/10.1155/2014/931372 http://www.hindawi.com/journals/bmri/2014/931372/abs/ |
| الوسوم: |
إضافة وسم
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| _version_ | 1864513461018427392 |
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| author | Khalaf, Roy A. |
| author2 | Tokajian, Sima Bitar, Ibrahim Harastani, Houda |
| author2_role | author author author |
| author_facet | Khalaf, Roy A. Tokajian, Sima Bitar, Ibrahim Harastani, Houda |
| author_role | author |
| dc.creator.none.fl_str_mv | Khalaf, Roy A. Tokajian, Sima Bitar, Ibrahim Harastani, Houda |
| dc.date.none.fl_str_mv | 2014 2016-03-21T07:34:15Z 2016-03-21T07:34:15Z 2016-03-21 |
| dc.identifier.none.fl_str_mv | 2314-6133 http://hdl.handle.net/10725/3365 http://dx.doi.org/10.1155/2014/931372 Bitar, I., Khalaf, R. A., Harastani, H., & Tokajian, S. (2014). Identification, Typing, Antifungal Resistance Profile, and Biofilm Formation of Candida albicans Isolates from Lebanese Hospital Patients. BioMed research international, 2014. http://www.hindawi.com/journals/bmri/2014/931372/abs/ |
| dc.language.none.fl_str_mv | en |
| dc.relation.none.fl_str_mv | BioMed Research International |
| dc.rights.*.fl_str_mv | info:eu-repo/semantics/openAccess |
| dc.title.none.fl_str_mv | Identification, Typing, Antifungal Resistance Profile, and Biofilm Formation of Candida albicans Isolates from Lebanese Hospital Patients |
| dc.type.none.fl_str_mv | Article info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article |
| description | As leading opportunistic fungal pathogens identification and subtyping of Candida species are crucial in recognizing outbreaks of infection, recognizing particularly virulent strains, and detecting the emergence of drug resistant strains. In this study our objective was to compare identification of Candida albicans by the hospitals through the use of conventional versus identification based on the ITS (Internal Transcribed Spacer) and to assess biofilm forming capabilities, drug resistance patterns and correlate these with MLST typing. ITS typing revealed a 21.2% hospital misidentification rate. Multidrug resistance to three drugs out of four tested was detected within 25% of the isolates raising concerns about the followed treatment regimens. Drug resistant strains as well as biofilm formers were phylogenetically related, with some isolates with significant biofilm forming capabilities being correlated to those that were multidrug resistant. Such isolates were grouped closely together in a neighbor-joining tree generated by MLST typing indicating phylogenetic relatedness, microevolution, or recurrent infection. In conclusion, this pilot study gives much needed insight concerning C. albicans isolates circulating in Lebanese hospitals and is the first study of its kind correlating biofilm formation, antifungal resistance, and evolutionary relatedness. |
| eu_rights_str_mv | openAccess |
| format | article |
| id | LAURepo_1bb57017dece26b27456ce8a321a68a3 |
| identifier_str_mv | 2314-6133 Bitar, I., Khalaf, R. A., Harastani, H., & Tokajian, S. (2014). Identification, Typing, Antifungal Resistance Profile, and Biofilm Formation of Candida albicans Isolates from Lebanese Hospital Patients. BioMed research international, 2014. |
| language_invalid_str_mv | en |
| network_acronym_str | LAURepo |
| network_name_str | Lebanese American University repository |
| oai_identifier_str | oai:laur.lau.edu.lb:10725/3365 |
| publishDate | 2014 |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| spelling | Identification, Typing, Antifungal Resistance Profile, and Biofilm Formation of Candida albicans Isolates from Lebanese Hospital PatientsKhalaf, Roy A.Tokajian, SimaBitar, IbrahimHarastani, HoudaAs leading opportunistic fungal pathogens identification and subtyping of Candida species are crucial in recognizing outbreaks of infection, recognizing particularly virulent strains, and detecting the emergence of drug resistant strains. In this study our objective was to compare identification of Candida albicans by the hospitals through the use of conventional versus identification based on the ITS (Internal Transcribed Spacer) and to assess biofilm forming capabilities, drug resistance patterns and correlate these with MLST typing. ITS typing revealed a 21.2% hospital misidentification rate. Multidrug resistance to three drugs out of four tested was detected within 25% of the isolates raising concerns about the followed treatment regimens. Drug resistant strains as well as biofilm formers were phylogenetically related, with some isolates with significant biofilm forming capabilities being correlated to those that were multidrug resistant. Such isolates were grouped closely together in a neighbor-joining tree generated by MLST typing indicating phylogenetic relatedness, microevolution, or recurrent infection. In conclusion, this pilot study gives much needed insight concerning C. albicans isolates circulating in Lebanese hospitals and is the first study of its kind correlating biofilm formation, antifungal resistance, and evolutionary relatedness.PublishedN/A2016-03-21T07:34:15Z2016-03-21T07:34:15Z20142016-03-21Articleinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article2314-6133http://hdl.handle.net/10725/3365http://dx.doi.org/10.1155/2014/931372Bitar, I., Khalaf, R. A., Harastani, H., & Tokajian, S. (2014). Identification, Typing, Antifungal Resistance Profile, and Biofilm Formation of Candida albicans Isolates from Lebanese Hospital Patients. BioMed research international, 2014.http://www.hindawi.com/journals/bmri/2014/931372/abs/enBioMed Research Internationalinfo:eu-repo/semantics/openAccessoai:laur.lau.edu.lb:10725/33652018-05-23T05:57:00Z |
| spellingShingle | Identification, Typing, Antifungal Resistance Profile, and Biofilm Formation of Candida albicans Isolates from Lebanese Hospital Patients Khalaf, Roy A. |
| status_str | publishedVersion |
| title | Identification, Typing, Antifungal Resistance Profile, and Biofilm Formation of Candida albicans Isolates from Lebanese Hospital Patients |
| title_full | Identification, Typing, Antifungal Resistance Profile, and Biofilm Formation of Candida albicans Isolates from Lebanese Hospital Patients |
| title_fullStr | Identification, Typing, Antifungal Resistance Profile, and Biofilm Formation of Candida albicans Isolates from Lebanese Hospital Patients |
| title_full_unstemmed | Identification, Typing, Antifungal Resistance Profile, and Biofilm Formation of Candida albicans Isolates from Lebanese Hospital Patients |
| title_short | Identification, Typing, Antifungal Resistance Profile, and Biofilm Formation of Candida albicans Isolates from Lebanese Hospital Patients |
| title_sort | Identification, Typing, Antifungal Resistance Profile, and Biofilm Formation of Candida albicans Isolates from Lebanese Hospital Patients |
| url | http://hdl.handle.net/10725/3365 http://dx.doi.org/10.1155/2014/931372 http://www.hindawi.com/journals/bmri/2014/931372/abs/ |