Drug-free phototherapy of superficial tumors
Visible light has long been recognized as a treatment for many diseases and an essential component of photo-induced chemotherapy. While previous data proved its inherent cytotoxicity, this study is the first to explore the use of a commercially available, high-intensity white LED light (24.5 mW.cm−2...
محفوظ في:
| المؤلف الرئيسي: | |
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| مؤلفون آخرون: | , , , , |
| التنسيق: | article |
| منشور في: |
2021
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| الوصول للمادة أونلاين: | http://hdl.handle.net/10725/14362 https://doi.org/10.1016/j.jphotobiol.2021.112324 http://libraries.lau.edu.lb/research/laur/terms-of-use/articles.php https://www.sciencedirect.com/science/article/pii/S1011134421002037 |
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| _version_ | 1864513469283303424 |
|---|---|
| author | Mehanna, Stephanie |
| author2 | Mansour, Najwa Daher, Costantine F. Elias, Maria George Dagher, Carole Khnayzer, Rony S. |
| author2_role | author author author author author |
| author_facet | Mehanna, Stephanie Mansour, Najwa Daher, Costantine F. Elias, Maria George Dagher, Carole Khnayzer, Rony S. |
| author_role | author |
| dc.creator.none.fl_str_mv | Mehanna, Stephanie Mansour, Najwa Daher, Costantine F. Elias, Maria George Dagher, Carole Khnayzer, Rony S. |
| dc.date.none.fl_str_mv | 2021 2023-01-13T13:30:02Z 2023-01-13T13:30:02Z 2023-01-13 |
| dc.identifier.none.fl_str_mv | 1011-1344 http://hdl.handle.net/10725/14362 https://doi.org/10.1016/j.jphotobiol.2021.112324 Mehanna, S., Mansour, N., Daher, C. F., Elias, M. G., Dagher, C., & Khnayzer, R. S. (2021). Drug-free phototherapy of superficial tumors: White light at the end of the tunnel. Journal of Photochemistry and Photobiology B: Biology, 224, 112324. http://libraries.lau.edu.lb/research/laur/terms-of-use/articles.php https://www.sciencedirect.com/science/article/pii/S1011134421002037 |
| dc.language.none.fl_str_mv | en |
| dc.relation.none.fl_str_mv | Journal of Photochemistry and Photobiology B: Biology |
| dc.rights.*.fl_str_mv | info:eu-repo/semantics/openAccess |
| dc.title.none.fl_str_mv | Drug-free phototherapy of superficial tumors White light at the end of the tunnel |
| dc.type.none.fl_str_mv | Article info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article |
| description | Visible light has long been recognized as a treatment for many diseases and an essential component of photo-induced chemotherapy. While previous data proved its inherent cytotoxicity, this study is the first to explore the use of a commercially available, high-intensity white LED light (24.5 mW.cm−2) as a treatment for skin tumors. After a 9-h exposure in vitro, the viability of Human Malignant Melanoma cells (A375) decreased by around 70%. Western blot analysis suggested an apoptotic cell death confirmed by the upregulation of Bax, cleaved PARP/caspase-3/8, cytochrome c, and t-bid. Additionally, cellular ROS accumulation and DNA damage were induced upon irradiation with blue light. When tested on a DMBA/TPA skin carcinogenesis model, a 90-min exposure to white light thrice weekly resulted in a significant decrease in tumor volumes/incidence compared to control and cisplatin groups, and restored normal morphological features, as confirmed by histopathology. Toxicological evaluation of ight-treated animals indicated a 100% survival rate, no skin irritation, no signs of discomfort or changes in body weight/behavior, and no toxicities to vital organs. Although these results must be confirmed by further studies, this research showed that short-exposure by commercially available high-intensity white LED light irradiation may be a promising approach for the treatment of superficial malignancies. |
| eu_rights_str_mv | openAccess |
| format | article |
| id | LAURepo_1be58bb7da65a3069fa67354da871f5f |
| identifier_str_mv | 1011-1344 Mehanna, S., Mansour, N., Daher, C. F., Elias, M. G., Dagher, C., & Khnayzer, R. S. (2021). Drug-free phototherapy of superficial tumors: White light at the end of the tunnel. Journal of Photochemistry and Photobiology B: Biology, 224, 112324. |
| language_invalid_str_mv | en |
| network_acronym_str | LAURepo |
| network_name_str | Lebanese American University repository |
| oai_identifier_str | oai:laur.lau.edu.lb:10725/14362 |
| publishDate | 2021 |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| spelling | Drug-free phototherapy of superficial tumorsWhite light at the end of the tunnelMehanna, StephanieMansour, NajwaDaher, Costantine F.Elias, Maria GeorgeDagher, CaroleKhnayzer, Rony S.Visible light has long been recognized as a treatment for many diseases and an essential component of photo-induced chemotherapy. While previous data proved its inherent cytotoxicity, this study is the first to explore the use of a commercially available, high-intensity white LED light (24.5 mW.cm−2) as a treatment for skin tumors. After a 9-h exposure in vitro, the viability of Human Malignant Melanoma cells (A375) decreased by around 70%. Western blot analysis suggested an apoptotic cell death confirmed by the upregulation of Bax, cleaved PARP/caspase-3/8, cytochrome c, and t-bid. Additionally, cellular ROS accumulation and DNA damage were induced upon irradiation with blue light. When tested on a DMBA/TPA skin carcinogenesis model, a 90-min exposure to white light thrice weekly resulted in a significant decrease in tumor volumes/incidence compared to control and cisplatin groups, and restored normal morphological features, as confirmed by histopathology. Toxicological evaluation of ight-treated animals indicated a 100% survival rate, no skin irritation, no signs of discomfort or changes in body weight/behavior, and no toxicities to vital organs. Although these results must be confirmed by further studies, this research showed that short-exposure by commercially available high-intensity white LED light irradiation may be a promising approach for the treatment of superficial malignancies.Published2023-01-13T13:30:02Z2023-01-13T13:30:02Z20212023-01-13Articleinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1011-1344http://hdl.handle.net/10725/14362https://doi.org/10.1016/j.jphotobiol.2021.112324Mehanna, S., Mansour, N., Daher, C. F., Elias, M. G., Dagher, C., & Khnayzer, R. S. (2021). Drug-free phototherapy of superficial tumors: White light at the end of the tunnel. Journal of Photochemistry and Photobiology B: Biology, 224, 112324.http://libraries.lau.edu.lb/research/laur/terms-of-use/articles.phphttps://www.sciencedirect.com/science/article/pii/S1011134421002037enJournal of Photochemistry and Photobiology B: Biologyinfo:eu-repo/semantics/openAccessoai:laur.lau.edu.lb:10725/143622023-01-16T13:06:20Z |
| spellingShingle | Drug-free phototherapy of superficial tumors Mehanna, Stephanie |
| status_str | publishedVersion |
| title | Drug-free phototherapy of superficial tumors |
| title_full | Drug-free phototherapy of superficial tumors |
| title_fullStr | Drug-free phototherapy of superficial tumors |
| title_full_unstemmed | Drug-free phototherapy of superficial tumors |
| title_short | Drug-free phototherapy of superficial tumors |
| title_sort | Drug-free phototherapy of superficial tumors |
| url | http://hdl.handle.net/10725/14362 https://doi.org/10.1016/j.jphotobiol.2021.112324 http://libraries.lau.edu.lb/research/laur/terms-of-use/articles.php https://www.sciencedirect.com/science/article/pii/S1011134421002037 |