The pro-apoptotic effect of kefir in malignant T-lymphocytes involves a p53 dependent pathway
Context: Kefir is an important probiotic that contains many bioactive ingredients which give it unique health benefits. Several studies have reported the anti-tumor effect of kefir on certain types of cancer. Adult lymphoblastic Leukemia (ALL) characterized by the continuous division of white blood...
محفوظ في:
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| مؤلفون آخرون: | , , |
| التنسيق: | article |
| منشور في: |
2013
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| الوصول للمادة أونلاين: | http://hdl.handle.net/10725/8070 https://doi.org/10.1016/j.clml.2013.07.062 http://libraries.lau.edu.lb/research/laur/terms-of-use/articles.php https://www.clinical-lymphoma-myeloma-leukemia.com/article/S2152-2650(13)00304-2/abstract |
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| الملخص: | Context: Kefir is an important probiotic that contains many bioactive ingredients which give it unique health benefits. Several studies have reported the anti-tumor effect of kefir on certain types of cancer. Adult lymphoblastic Leukemia (ALL) characterized by the continuous division of white blood cells in the bone marrow, and Adult T Cell Leukemia (ATL) a malignancy of activated T lymphocytes caused by Human T-cell Lymphotropic Virus type 1 (HTLV-1) will be addressed in this study. Objective: The aim of this study is to inspect the effect of a cell-free fraction of kefir on the induction of apoptosis in two malignant T-lymphocyte cell lines. Methods: CEM cells (HTLV-1 negative) and HUT-201 cells (HTLV-1 positive) leukemic cells were incubated with different concentrations of a cell free fraction of kefir. Apoptosis induction was measured using Cell Death Elisa, as well as annexin labeling followed by flow cytometry . The molecular mechanism underlying apoptosis induction was further investigated by western blots to assess the expression of the proapoptotic protein Bax, the anti-apoptotic protein Bcl-2, and p53. Results: Previous work in our lab has shown the anti-proliferative effect of kefir in both HTLV-1 negative as well as HTLV-1 positive leukemic cell lines with no significant cytotoxic effect on normal lymphocytes. We now report a dose- and time-dependent proapoptotic effect of kefir on both cell lines using Cell Death Elisa; this was further confirmed using annexin staining followed by flow cytomtery analysis. Furthermore, kefir treatment did not alter p53 expression, even though it caused an up-regulation of Bax proteins and a down-regulation of Bcl-2 in a dose and time dependent manner in both cell lines. This confirms the pro-apoptotic effect of kefir via a p53-independent mechanism in HTLV-1 negative and HTLV-1 positive cell lines. Conclusion: These results suggest that milk kefir is a promising food component in terms of cancer prevention. Further studies should aim at identifying the active ingredient in kefir responsible for its anti-tumor effect, and assessing its activity in vivo. |
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