The vitamin E derivative gamma tocotrienol promotes anti-tumor effects in acute myeloid leukemia cell lines. (c2019)
Acute Myeloid Leukemia (AML) is a type of cancer characterized by the formation of faulty myelogenous cells with morphological heterogeneity and cytogenic aberrations leading to a total loss of their function. In the midst of the struggle to find an effective and safe AML treatment, vitamin E deriva...
Saved in:
| Main Author: | |
|---|---|
| Format: | masterThesis |
| Published: |
2019
|
| Subjects: | |
| Online Access: | http://hdl.handle.net/10725/11496 https://doi.org/10.26756/th.2019.131 http://libraries.lau.edu.lb/research/laur/terms-of-use/thesis.php |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1864513488802545664 |
|---|---|
| author | Mohamad, Maya Riad |
| author_facet | Mohamad, Maya Riad |
| author_role | author |
| dc.creator.none.fl_str_mv | Mohamad, Maya Riad |
| dc.date.none.fl_str_mv | 2019-11-06T11:00:28Z 2019-11-06T11:00:28Z 2019 2019-11-06 2019-08-05 |
| dc.identifier.none.fl_str_mv | http://hdl.handle.net/10725/11496 https://doi.org/10.26756/th.2019.131 http://libraries.lau.edu.lb/research/laur/terms-of-use/thesis.php |
| dc.language.none.fl_str_mv | en |
| dc.publisher.none.fl_str_mv | Lebanese American University |
| dc.rights.*.fl_str_mv | info:eu-repo/semantics/openAccess |
| dc.subject.none.fl_str_mv | Dissertations, Academic Lebanese American University -- Dissertations Drug resistance in cancer cells Acute myeloid leukemia -- Treatment Tocotrienol |
| dc.title.none.fl_str_mv | The vitamin E derivative gamma tocotrienol promotes anti-tumor effects in acute myeloid leukemia cell lines. (c2019) |
| dc.type.none.fl_str_mv | Thesis info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/masterThesis |
| description | Acute Myeloid Leukemia (AML) is a type of cancer characterized by the formation of faulty myelogenous cells with morphological heterogeneity and cytogenic aberrations leading to a total loss of their function. In the midst of the struggle to find an effective and safe AML treatment, vitamin E derivatives, mainly tocopherols were previously considered as potential anti-tumor compounds. Recently, other isoforms of vitamin E, namely tocotrienols have been proposed as potential potent anti-cancerous agents, displaying promising therapeutic effects in different cancer types. The aim of the present study is to investigate the anti-cancerous effect of γ-tocotrienol, through examining the extent of its effects on the growth of human-derived AML cell lines in vitro, and deciphering the underlying molecular mechanisms. This was achieved by performing MTS cell viability assays, propidium iodide (PI) based cell cycle analysis, flow cytometry-based annexin V/PI apoptosis staining assay, cell death detection ELISA, western immunoblotting, and reactive oxygen species detection assay. Analysis of our results show that γ-tocotrienol exhibits time and dose-dependent anti-proliferative, pro-apoptotic and antioxidant effects on U937 and KG-1 cell lines, through the upregulation of several proteins involved in the intrinsic apoptotic pathway. |
| eu_rights_str_mv | openAccess |
| format | masterThesis |
| id | LAURepo_201e800cc4ee11dfd8d414cfe8506bd9 |
| language_invalid_str_mv | en |
| network_acronym_str | LAURepo |
| network_name_str | Lebanese American University repository |
| oai_identifier_str | oai:laur.lau.edu.lb:10725/11496 |
| publishDate | 2019 |
| publisher.none.fl_str_mv | Lebanese American University |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| spelling | The vitamin E derivative gamma tocotrienol promotes anti-tumor effects in acute myeloid leukemia cell lines. (c2019)Mohamad, Maya RiadDissertations, AcademicLebanese American University -- DissertationsDrug resistance in cancer cellsAcute myeloid leukemia -- TreatmentTocotrienolAcute Myeloid Leukemia (AML) is a type of cancer characterized by the formation of faulty myelogenous cells with morphological heterogeneity and cytogenic aberrations leading to a total loss of their function. In the midst of the struggle to find an effective and safe AML treatment, vitamin E derivatives, mainly tocopherols were previously considered as potential anti-tumor compounds. Recently, other isoforms of vitamin E, namely tocotrienols have been proposed as potential potent anti-cancerous agents, displaying promising therapeutic effects in different cancer types. The aim of the present study is to investigate the anti-cancerous effect of γ-tocotrienol, through examining the extent of its effects on the growth of human-derived AML cell lines in vitro, and deciphering the underlying molecular mechanisms. This was achieved by performing MTS cell viability assays, propidium iodide (PI) based cell cycle analysis, flow cytometry-based annexin V/PI apoptosis staining assay, cell death detection ELISA, western immunoblotting, and reactive oxygen species detection assay. Analysis of our results show that γ-tocotrienol exhibits time and dose-dependent anti-proliferative, pro-apoptotic and antioxidant effects on U937 and KG-1 cell lines, through the upregulation of several proteins involved in the intrinsic apoptotic pathway.N/A1 hard copy: xvi, 92 leaves; col. ill.; 31 cm. available at RNL.Bibliography: leaves 77-92.Lebanese American University2019-11-06T11:00:28Z2019-11-06T11:00:28Z20192019-11-062019-08-05Thesisinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesishttp://hdl.handle.net/10725/11496https://doi.org/10.26756/th.2019.131http://libraries.lau.edu.lb/research/laur/terms-of-use/thesis.phpeninfo:eu-repo/semantics/openAccessoai:laur.lau.edu.lb:10725/114962021-03-19T10:47:38Z |
| spellingShingle | The vitamin E derivative gamma tocotrienol promotes anti-tumor effects in acute myeloid leukemia cell lines. (c2019) Mohamad, Maya Riad Dissertations, Academic Lebanese American University -- Dissertations Drug resistance in cancer cells Acute myeloid leukemia -- Treatment Tocotrienol |
| status_str | publishedVersion |
| title | The vitamin E derivative gamma tocotrienol promotes anti-tumor effects in acute myeloid leukemia cell lines. (c2019) |
| title_full | The vitamin E derivative gamma tocotrienol promotes anti-tumor effects in acute myeloid leukemia cell lines. (c2019) |
| title_fullStr | The vitamin E derivative gamma tocotrienol promotes anti-tumor effects in acute myeloid leukemia cell lines. (c2019) |
| title_full_unstemmed | The vitamin E derivative gamma tocotrienol promotes anti-tumor effects in acute myeloid leukemia cell lines. (c2019) |
| title_short | The vitamin E derivative gamma tocotrienol promotes anti-tumor effects in acute myeloid leukemia cell lines. (c2019) |
| title_sort | The vitamin E derivative gamma tocotrienol promotes anti-tumor effects in acute myeloid leukemia cell lines. (c2019) |
| topic | Dissertations, Academic Lebanese American University -- Dissertations Drug resistance in cancer cells Acute myeloid leukemia -- Treatment Tocotrienol |
| url | http://hdl.handle.net/10725/11496 https://doi.org/10.26756/th.2019.131 http://libraries.lau.edu.lb/research/laur/terms-of-use/thesis.php |