The Effect of HuArgI (Co)-PEG5000 on The Migration of Ovarian Cancer
Ovarian cancer being the first cause of death in gynecological diseases, paves the way towards increasing the search for a targeted therapy that could control the metastasis of a late-stage diagnosed ovarian cancer. HuArgI (Co)-PEG5000 is a potential drug to target cancers that depend on exogenous s...
محفوظ في:
| المؤلف الرئيسي: | |
|---|---|
| التنسيق: | masterThesis |
| منشور في: |
2020
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| الموضوعات: | |
| الوصول للمادة أونلاين: | http://hdl.handle.net/10725/13842 https://doi.org/10.26756/th.2022.368 http://libraries.lau.edu.lb/research/laur/terms-of-use/thesis.php |
| الوسوم: |
إضافة وسم
لا توجد وسوم, كن أول من يضع وسما على هذه التسجيلة!
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| _version_ | 1864513468312322048 |
|---|---|
| author | El Mais, Nour |
| author_facet | El Mais, Nour |
| author_role | author |
| dc.creator.none.fl_str_mv | El Mais, Nour |
| dc.date.none.fl_str_mv | 2020 2020-05-22 2022-07-20T08:43:07Z 2022-07-20T08:43:07Z |
| dc.identifier.none.fl_str_mv | http://hdl.handle.net/10725/13842 https://doi.org/10.26756/th.2022.368 http://libraries.lau.edu.lb/research/laur/terms-of-use/thesis.php |
| dc.language.none.fl_str_mv | en |
| dc.publisher.none.fl_str_mv | Lebanese American University |
| dc.rights.*.fl_str_mv | info:eu-repo/semantics/openAccess |
| dc.subject.none.fl_str_mv | Ovaries -- Cancer -- Treatment Cancer cells -- Motility Arginine -- Therapeutic use Lebanese American University -- Dissertations Dissertations, Academic |
| dc.title.none.fl_str_mv | The Effect of HuArgI (Co)-PEG5000 on The Migration of Ovarian Cancer |
| dc.type.none.fl_str_mv | Thesis info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/masterThesis |
| description | Ovarian cancer being the first cause of death in gynecological diseases, paves the way towards increasing the search for a targeted therapy that could control the metastasis of a late-stage diagnosed ovarian cancer. HuArgI (Co)-PEG5000 is a potential drug to target cancers that depend on exogenous sources of arginine and cannot synthesize their own. In this study, we hypothesized that HuArgI (Co)- PEG5000 can decrease the migration of ovarian cancer cells. Our results show a decrease in the migratory rate of the cells and their rate in wound closure. The second thing we looked into was the adhesion of those cells and the formation of focal adhesions. The number and area of focal adhesions were quantified and the results show a decrease in the area and number of focal adhesions upon treating with HuArgI (Co)-PEG5000. Finally, we looked at the effect of HuArgI (Co)-PEG5000 on 3D motility of the ovarian cancer cell line, using a collagen based assay. It showed that SKOV3 cell line does not show significant invasiveness to collagen. In conclusion, we noticed an effect on ovarian cancer by treating the cells with HuArgI (Co)-PEG5000 which opens the way for further studies on the effect of arginine deprivation and autophagy induction on the migration of ovarian cancer. |
| eu_rights_str_mv | openAccess |
| format | masterThesis |
| id | LAURepo_234fd8787d0fc7fd0201762c846deb61 |
| language_invalid_str_mv | en |
| network_acronym_str | LAURepo |
| network_name_str | Lebanese American University repository |
| oai_identifier_str | oai:laur.lau.edu.lb:10725/13842 |
| publishDate | 2020 |
| publisher.none.fl_str_mv | Lebanese American University |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| spelling | The Effect of HuArgI (Co)-PEG5000 on The Migration of Ovarian CancerEl Mais, NourOvaries -- Cancer -- TreatmentCancer cells -- MotilityArginine -- Therapeutic useLebanese American University -- DissertationsDissertations, AcademicOvarian cancer being the first cause of death in gynecological diseases, paves the way towards increasing the search for a targeted therapy that could control the metastasis of a late-stage diagnosed ovarian cancer. HuArgI (Co)-PEG5000 is a potential drug to target cancers that depend on exogenous sources of arginine and cannot synthesize their own. In this study, we hypothesized that HuArgI (Co)- PEG5000 can decrease the migration of ovarian cancer cells. Our results show a decrease in the migratory rate of the cells and their rate in wound closure. The second thing we looked into was the adhesion of those cells and the formation of focal adhesions. The number and area of focal adhesions were quantified and the results show a decrease in the area and number of focal adhesions upon treating with HuArgI (Co)-PEG5000. Finally, we looked at the effect of HuArgI (Co)-PEG5000 on 3D motility of the ovarian cancer cell line, using a collagen based assay. It showed that SKOV3 cell line does not show significant invasiveness to collagen. In conclusion, we noticed an effect on ovarian cancer by treating the cells with HuArgI (Co)-PEG5000 which opens the way for further studies on the effect of arginine deprivation and autophagy induction on the migration of ovarian cancer.1 online resource (xi, 60 leaves): col. ill.Bibliography: leaf 48-50.Lebanese American University2022-07-20T08:43:07Z2022-07-20T08:43:07Z20202020-05-22Thesisinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesishttp://hdl.handle.net/10725/13842https://doi.org/10.26756/th.2022.368http://libraries.lau.edu.lb/research/laur/terms-of-use/thesis.phpeninfo:eu-repo/semantics/openAccessoai:laur.lau.edu.lb:10725/138422022-07-20T08:47:15Z |
| spellingShingle | The Effect of HuArgI (Co)-PEG5000 on The Migration of Ovarian Cancer El Mais, Nour Ovaries -- Cancer -- Treatment Cancer cells -- Motility Arginine -- Therapeutic use Lebanese American University -- Dissertations Dissertations, Academic |
| status_str | publishedVersion |
| title | The Effect of HuArgI (Co)-PEG5000 on The Migration of Ovarian Cancer |
| title_full | The Effect of HuArgI (Co)-PEG5000 on The Migration of Ovarian Cancer |
| title_fullStr | The Effect of HuArgI (Co)-PEG5000 on The Migration of Ovarian Cancer |
| title_full_unstemmed | The Effect of HuArgI (Co)-PEG5000 on The Migration of Ovarian Cancer |
| title_short | The Effect of HuArgI (Co)-PEG5000 on The Migration of Ovarian Cancer |
| title_sort | The Effect of HuArgI (Co)-PEG5000 on The Migration of Ovarian Cancer |
| topic | Ovaries -- Cancer -- Treatment Cancer cells -- Motility Arginine -- Therapeutic use Lebanese American University -- Dissertations Dissertations, Academic |
| url | http://hdl.handle.net/10725/13842 https://doi.org/10.26756/th.2022.368 http://libraries.lau.edu.lb/research/laur/terms-of-use/thesis.php |