Adjuvant Epigenetic Therapy of Decitabine and Suberoylanilide Hydroxamic Acid Exerts Anti-Neoplastic Effects in Acute Myeloid Leukemia Cells
Atypical epigenetic processes including histone acetylation and DNA methylation have been identified as a fundamental theme in hematologic malignancies. Such mechanisms modify gene expression and prompt, in part at least, the initiation and progression of several malignancies including acute myeloid...
Saved in:
| Main Author: | |
|---|---|
| Other Authors: | , , , |
| Format: | article |
| Published: |
2019
|
| Online Access: | http://hdl.handle.net/10725/11976 https://doi.org/10.3390/cells8121480 http://libraries.lau.edu.lb/research/laur/terms-of-use/articles.php https://www.mdpi.com/2073-4409/8/12/1480 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1864513489658183680 |
|---|---|
| author | Abou Najem, Sonia |
| author2 | Khawaja, Ghada Hodroj, Mohammad Hassan Babikian, Patil Rizk, Sandra |
| author2_role | author author author author |
| author_facet | Abou Najem, Sonia Khawaja, Ghada Hodroj, Mohammad Hassan Babikian, Patil Rizk, Sandra |
| author_role | author |
| dc.creator.none.fl_str_mv | Abou Najem, Sonia Khawaja, Ghada Hodroj, Mohammad Hassan Babikian, Patil Rizk, Sandra |
| dc.date.none.fl_str_mv | 2019 2020-07-15T09:09:04Z 2020-07-15T09:09:04Z 2020-07-15 |
| dc.identifier.none.fl_str_mv | 2073-4409 http://hdl.handle.net/10725/11976 https://doi.org/10.3390/cells8121480 Abou Najem, S., Khawaja, G., Hodroj, M. H., Babikian, P., & Rizk, S. (2019). Adjuvant epigenetic therapy of decitabine and suberoylanilide hydroxamic acid exerts anti-neoplastic effects in acute myeloid leukemia cells. Cells, 8(12), 1-23. http://libraries.lau.edu.lb/research/laur/terms-of-use/articles.php https://www.mdpi.com/2073-4409/8/12/1480 |
| dc.language.none.fl_str_mv | en |
| dc.relation.none.fl_str_mv | Cells |
| dc.rights.*.fl_str_mv | info:eu-repo/semantics/openAccess |
| dc.title.none.fl_str_mv | Adjuvant Epigenetic Therapy of Decitabine and Suberoylanilide Hydroxamic Acid Exerts Anti-Neoplastic Effects in Acute Myeloid Leukemia Cells |
| dc.type.none.fl_str_mv | Article info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article |
| description | Atypical epigenetic processes including histone acetylation and DNA methylation have been identified as a fundamental theme in hematologic malignancies. Such mechanisms modify gene expression and prompt, in part at least, the initiation and progression of several malignancies including acute myeloid leukemia. In the current study we determined the effects of treating KG-1 and U937 acute myeloid leukemia (AML) cells, in vitro, with the HDAC inhibitor, suberoylanilide hydroxamic acid (SAHA), or with a DNMT inhibitor, decitabine (DAC), or their combination, on cell proliferation, cell cycle progression, apoptosis, and expression of apoptosis-related proteins. Each of SAHA and DAC attenuated cell proliferation and induced cell cycle arrest and apoptotic cell death of KG-1 and U937 cell lines. Besides, their sequential combination improved the obtained anti-neoplastic effect: significant augmentation of growth inhibition and apoptosis induction as compared to cells treated with either drug alone. This effect was featured by the upregulated expression of Bax, cytochrome c1, p21, and cleaved caspases 8, 9, and 3, signifying the activation of both the intrinsic and extrinsic pathways of apoptosis. The sequential combination of SAHA and DAC causes a profound antitumorigenic effect in AML cell lines by inducing the expression of tumor suppressor genes |
| eu_rights_str_mv | openAccess |
| format | article |
| id | LAURepo_262e85d51ceaf4721fb6ff2ed2e63ba9 |
| identifier_str_mv | 2073-4409 Abou Najem, S., Khawaja, G., Hodroj, M. H., Babikian, P., & Rizk, S. (2019). Adjuvant epigenetic therapy of decitabine and suberoylanilide hydroxamic acid exerts anti-neoplastic effects in acute myeloid leukemia cells. Cells, 8(12), 1-23. |
| language_invalid_str_mv | en |
| network_acronym_str | LAURepo |
| network_name_str | Lebanese American University repository |
| oai_identifier_str | oai:laur.lau.edu.lb:10725/11976 |
| publishDate | 2019 |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| spelling | Adjuvant Epigenetic Therapy of Decitabine and Suberoylanilide Hydroxamic Acid Exerts Anti-Neoplastic Effects in Acute Myeloid Leukemia CellsAbou Najem, SoniaKhawaja, GhadaHodroj, Mohammad HassanBabikian, PatilRizk, SandraAtypical epigenetic processes including histone acetylation and DNA methylation have been identified as a fundamental theme in hematologic malignancies. Such mechanisms modify gene expression and prompt, in part at least, the initiation and progression of several malignancies including acute myeloid leukemia. In the current study we determined the effects of treating KG-1 and U937 acute myeloid leukemia (AML) cells, in vitro, with the HDAC inhibitor, suberoylanilide hydroxamic acid (SAHA), or with a DNMT inhibitor, decitabine (DAC), or their combination, on cell proliferation, cell cycle progression, apoptosis, and expression of apoptosis-related proteins. Each of SAHA and DAC attenuated cell proliferation and induced cell cycle arrest and apoptotic cell death of KG-1 and U937 cell lines. Besides, their sequential combination improved the obtained anti-neoplastic effect: significant augmentation of growth inhibition and apoptosis induction as compared to cells treated with either drug alone. This effect was featured by the upregulated expression of Bax, cytochrome c1, p21, and cleaved caspases 8, 9, and 3, signifying the activation of both the intrinsic and extrinsic pathways of apoptosis. The sequential combination of SAHA and DAC causes a profound antitumorigenic effect in AML cell lines by inducing the expression of tumor suppressor genesPublishedN/A2020-07-15T09:09:04Z2020-07-15T09:09:04Z20192020-07-15Articleinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article2073-4409http://hdl.handle.net/10725/11976https://doi.org/10.3390/cells8121480Abou Najem, S., Khawaja, G., Hodroj, M. H., Babikian, P., & Rizk, S. (2019). Adjuvant epigenetic therapy of decitabine and suberoylanilide hydroxamic acid exerts anti-neoplastic effects in acute myeloid leukemia cells. Cells, 8(12), 1-23.http://libraries.lau.edu.lb/research/laur/terms-of-use/articles.phphttps://www.mdpi.com/2073-4409/8/12/1480enCellsinfo:eu-repo/semantics/openAccessoai:laur.lau.edu.lb:10725/119762021-03-19T10:47:40Z |
| spellingShingle | Adjuvant Epigenetic Therapy of Decitabine and Suberoylanilide Hydroxamic Acid Exerts Anti-Neoplastic Effects in Acute Myeloid Leukemia Cells Abou Najem, Sonia |
| status_str | publishedVersion |
| title | Adjuvant Epigenetic Therapy of Decitabine and Suberoylanilide Hydroxamic Acid Exerts Anti-Neoplastic Effects in Acute Myeloid Leukemia Cells |
| title_full | Adjuvant Epigenetic Therapy of Decitabine and Suberoylanilide Hydroxamic Acid Exerts Anti-Neoplastic Effects in Acute Myeloid Leukemia Cells |
| title_fullStr | Adjuvant Epigenetic Therapy of Decitabine and Suberoylanilide Hydroxamic Acid Exerts Anti-Neoplastic Effects in Acute Myeloid Leukemia Cells |
| title_full_unstemmed | Adjuvant Epigenetic Therapy of Decitabine and Suberoylanilide Hydroxamic Acid Exerts Anti-Neoplastic Effects in Acute Myeloid Leukemia Cells |
| title_short | Adjuvant Epigenetic Therapy of Decitabine and Suberoylanilide Hydroxamic Acid Exerts Anti-Neoplastic Effects in Acute Myeloid Leukemia Cells |
| title_sort | Adjuvant Epigenetic Therapy of Decitabine and Suberoylanilide Hydroxamic Acid Exerts Anti-Neoplastic Effects in Acute Myeloid Leukemia Cells |
| url | http://hdl.handle.net/10725/11976 https://doi.org/10.3390/cells8121480 http://libraries.lau.edu.lb/research/laur/terms-of-use/articles.php https://www.mdpi.com/2073-4409/8/12/1480 |