Binding of 3-aminoacridinium and 7-aminoquinolinium monocations to double-stranded DNA
The complexations of the singly charged cations of 3-aminoacridine and 7-aminoquinoline by double-stranded, calf thymus DNA were studied by electronic absorption spectrophotometry. At high ratios of total DNA phosphate concentration to total probe concentration (the region associated with intercalat...
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1978
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| Online Access: | http://hdl.handle.net/10725/6347 http://dx.doi.org/10.1002/jps.2600670123 http://libraries.lau.edu.lb/research/laur/terms-of-use/articles.php http://onlinelibrary.wiley.com/doi/10.1002/jps.2600670123/full |
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| _version_ | 1864513479727120384 |
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| author | Capomacchia, Anthony C. |
| author2 | Schulman, Stephen G. |
| author2_role | author |
| author_facet | Capomacchia, Anthony C. Schulman, Stephen G. |
| author_role | author |
| dc.creator.none.fl_str_mv | Capomacchia, Anthony C. Schulman, Stephen G. |
| dc.date.none.fl_str_mv | 1978 2017-10-18T08:46:40Z 2017-10-18T08:46:40Z 2017-10-18 |
| dc.identifier.none.fl_str_mv | 1520-6017 http://hdl.handle.net/10725/6347 http://dx.doi.org/10.1002/jps.2600670123 Capomacchia, A. C., & Schulman, S. G. (1978). Binding of 3‐aminoacridinium and 7‐aminoquinolinium monocations to double‐stranded DNA: Evidence for nonlinear binding isotherm in intercalative region. Journal of pharmaceutical sciences, 67(1), 89-94. http://libraries.lau.edu.lb/research/laur/terms-of-use/articles.php http://onlinelibrary.wiley.com/doi/10.1002/jps.2600670123/full |
| dc.language.none.fl_str_mv | en |
| dc.relation.none.fl_str_mv | Journal of Pharmaceutical Sciences |
| dc.rights.*.fl_str_mv | info:eu-repo/semantics/openAccess |
| dc.title.none.fl_str_mv | Binding of 3-aminoacridinium and 7-aminoquinolinium monocations to double-stranded DNA evidence for nonlinear binding isotherm in intercalative region |
| dc.type.none.fl_str_mv | Article info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article |
| description | The complexations of the singly charged cations of 3-aminoacridine and 7-aminoquinoline by double-stranded, calf thymus DNA were studied by electronic absorption spectrophotometry. At high ratios of total DNA phosphate concentration to total probe concentration (the region associated with intercalative binding), four DNA phosphate units are associated with each bound monocation. However, a binding isotherm based on four DNA phosphate groups in a single binding site does not yield a reproducible equilibrium constant for the binding process. Rather, the sequestration of the monocations by two binding sites, each containing two DNA phosphate units, yields a satisfactory equilibrium expression. This result suggests that the intercalative mode of binding by DNA is not a simple one-step process, which is in agreement with previous kinetic studies. In the region of low DNA phosphate concentration to monocation concentration (the external binding region), the binding is described adequately by a linear isotherm. |
| eu_rights_str_mv | openAccess |
| format | article |
| id | LAURepo_27f83aa4b213e346a5dee5c438ce509a |
| identifier_str_mv | 1520-6017 Capomacchia, A. C., & Schulman, S. G. (1978). Binding of 3‐aminoacridinium and 7‐aminoquinolinium monocations to double‐stranded DNA: Evidence for nonlinear binding isotherm in intercalative region. Journal of pharmaceutical sciences, 67(1), 89-94. |
| language_invalid_str_mv | en |
| network_acronym_str | LAURepo |
| network_name_str | Lebanese American University repository |
| oai_identifier_str | oai:laur.lau.edu.lb:10725/6347 |
| publishDate | 1978 |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| spelling | Binding of 3-aminoacridinium and 7-aminoquinolinium monocations to double-stranded DNAevidence for nonlinear binding isotherm in intercalative regionCapomacchia, Anthony C.Schulman, Stephen G.The complexations of the singly charged cations of 3-aminoacridine and 7-aminoquinoline by double-stranded, calf thymus DNA were studied by electronic absorption spectrophotometry. At high ratios of total DNA phosphate concentration to total probe concentration (the region associated with intercalative binding), four DNA phosphate units are associated with each bound monocation. However, a binding isotherm based on four DNA phosphate groups in a single binding site does not yield a reproducible equilibrium constant for the binding process. Rather, the sequestration of the monocations by two binding sites, each containing two DNA phosphate units, yields a satisfactory equilibrium expression. This result suggests that the intercalative mode of binding by DNA is not a simple one-step process, which is in agreement with previous kinetic studies. In the region of low DNA phosphate concentration to monocation concentration (the external binding region), the binding is described adequately by a linear isotherm.PublishedN/A2017-10-18T08:46:40Z2017-10-18T08:46:40Z19782017-10-18Articleinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1520-6017http://hdl.handle.net/10725/6347http://dx.doi.org/10.1002/jps.2600670123Capomacchia, A. C., & Schulman, S. G. (1978). Binding of 3‐aminoacridinium and 7‐aminoquinolinium monocations to double‐stranded DNA: Evidence for nonlinear binding isotherm in intercalative region. Journal of pharmaceutical sciences, 67(1), 89-94.http://libraries.lau.edu.lb/research/laur/terms-of-use/articles.phphttp://onlinelibrary.wiley.com/doi/10.1002/jps.2600670123/fullenJournal of Pharmaceutical Sciencesinfo:eu-repo/semantics/openAccessoai:laur.lau.edu.lb:10725/63472021-03-19T10:03:26Z |
| spellingShingle | Binding of 3-aminoacridinium and 7-aminoquinolinium monocations to double-stranded DNA Capomacchia, Anthony C. |
| status_str | publishedVersion |
| title | Binding of 3-aminoacridinium and 7-aminoquinolinium monocations to double-stranded DNA |
| title_full | Binding of 3-aminoacridinium and 7-aminoquinolinium monocations to double-stranded DNA |
| title_fullStr | Binding of 3-aminoacridinium and 7-aminoquinolinium monocations to double-stranded DNA |
| title_full_unstemmed | Binding of 3-aminoacridinium and 7-aminoquinolinium monocations to double-stranded DNA |
| title_short | Binding of 3-aminoacridinium and 7-aminoquinolinium monocations to double-stranded DNA |
| title_sort | Binding of 3-aminoacridinium and 7-aminoquinolinium monocations to double-stranded DNA |
| url | http://hdl.handle.net/10725/6347 http://dx.doi.org/10.1002/jps.2600670123 http://libraries.lau.edu.lb/research/laur/terms-of-use/articles.php http://onlinelibrary.wiley.com/doi/10.1002/jps.2600670123/full |