A comparative study of the antiproliferative effect of kohlrabi and green cabbage on colorectal cancer cell lines in vitro
Experimental studies showed that phytochemicals from Brassicaceae Cruciferae vegetables possess anticarcinogenic properties. Two members of this family, namely cabbage (Brassica oleracea, group Capitata) and kohlrabi (Brassica oleracea, group Gongylodes) seem to share similar anticarcinogenic charac...
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2013
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| Online Access: | http://hdl.handle.net/10725/8082 http://libraries.lau.edu.lb/research/laur/terms-of-use/articles.php https://www.fasebj.org/doi/abs/10.1096/fasebj.27.1_supplement.639.13 |
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| _version_ | 1864513483852218368 |
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| author | Rizk, Sandra |
| author2 | Zarzour, Valia |
| author2_role | author |
| author_facet | Rizk, Sandra Zarzour, Valia |
| author_role | author |
| dc.creator.none.fl_str_mv | Rizk, Sandra Zarzour, Valia |
| dc.date.none.fl_str_mv | 2013 2018-06-22T06:24:00Z 2018-06-22T06:24:00Z 2018-06-22 |
| dc.identifier.none.fl_str_mv | 1530-6860 http://hdl.handle.net/10725/8082 Rizk, S., & Zarzour, V. (2013). A Comparative Study of the Antiproliferative Effect of Kohlrabi and Green Cabbage on Colorectal Cancer Cell Lines In Vitro. http://libraries.lau.edu.lb/research/laur/terms-of-use/articles.php https://www.fasebj.org/doi/abs/10.1096/fasebj.27.1_supplement.639.13 |
| dc.language.none.fl_str_mv | en |
| dc.relation.none.fl_str_mv | The FASEB Journal |
| dc.rights.*.fl_str_mv | info:eu-repo/semantics/openAccess |
| dc.title.none.fl_str_mv | A comparative study of the antiproliferative effect of kohlrabi and green cabbage on colorectal cancer cell lines in vitro |
| dc.type.none.fl_str_mv | Article info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article |
| description | Experimental studies showed that phytochemicals from Brassicaceae Cruciferae vegetables possess anticarcinogenic properties. Two members of this family, namely cabbage (Brassica oleracea, group Capitata) and kohlrabi (Brassica oleracea, group Gongylodes) seem to share similar anticarcinogenic characteristics. The aim of this study is to compare the effect of the ethanolic extracts of the edible parts of these two plants on the proliferation and apoptosis of HT-29 and Caco-2 colorectal cancer cell lines. The cytotoxicity of the extracts was assayed using the Trypan Blue Exclusion method and the IC50 was determined to be higher in kohlrabi compared to cabbage. Consequently, WST-1 assay was used to assess the effect of these plant extracts on the proliferation of colorectal cancer cell lines: both extracts exhibited a sharp anti-proliferative effect in a dose and time dependent manner. Analysis of cell DNA content by flow cytomtery revealed the ability of both kohlrabi and cabbage extracts to induce cell cycle arrest in HT-29 cells at the G phase and S phase in similar patterns. Furthermore both extracts promoted apoptosis in colorectal cancer cell lines. The apoptotic pathway seems to involve the pro-apoptotic protein Bax and the anti-apoptotic protein Bcl-2. As a conclusion, kohlrabi should be considered, like cabbage, among the potent anti-carcinogenic cruciferous vegetables. |
| eu_rights_str_mv | openAccess |
| format | article |
| id | LAURepo_2b40f2e168d6f52cdd253d1107ead6da |
| identifier_str_mv | 1530-6860 Rizk, S., & Zarzour, V. (2013). A Comparative Study of the Antiproliferative Effect of Kohlrabi and Green Cabbage on Colorectal Cancer Cell Lines In Vitro. |
| language_invalid_str_mv | en |
| network_acronym_str | LAURepo |
| network_name_str | Lebanese American University repository |
| oai_identifier_str | oai:laur.lau.edu.lb:10725/8082 |
| publishDate | 2013 |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| spelling | A comparative study of the antiproliferative effect of kohlrabi and green cabbage on colorectal cancer cell lines in vitroRizk, SandraZarzour, ValiaExperimental studies showed that phytochemicals from Brassicaceae Cruciferae vegetables possess anticarcinogenic properties. Two members of this family, namely cabbage (Brassica oleracea, group Capitata) and kohlrabi (Brassica oleracea, group Gongylodes) seem to share similar anticarcinogenic characteristics. The aim of this study is to compare the effect of the ethanolic extracts of the edible parts of these two plants on the proliferation and apoptosis of HT-29 and Caco-2 colorectal cancer cell lines. The cytotoxicity of the extracts was assayed using the Trypan Blue Exclusion method and the IC50 was determined to be higher in kohlrabi compared to cabbage. Consequently, WST-1 assay was used to assess the effect of these plant extracts on the proliferation of colorectal cancer cell lines: both extracts exhibited a sharp anti-proliferative effect in a dose and time dependent manner. Analysis of cell DNA content by flow cytomtery revealed the ability of both kohlrabi and cabbage extracts to induce cell cycle arrest in HT-29 cells at the G phase and S phase in similar patterns. Furthermore both extracts promoted apoptosis in colorectal cancer cell lines. The apoptotic pathway seems to involve the pro-apoptotic protein Bax and the anti-apoptotic protein Bcl-2. As a conclusion, kohlrabi should be considered, like cabbage, among the potent anti-carcinogenic cruciferous vegetables.PublishedN/A2018-06-22T06:24:00Z2018-06-22T06:24:00Z20132018-06-22Articleinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1530-6860http://hdl.handle.net/10725/8082Rizk, S., & Zarzour, V. (2013). A Comparative Study of the Antiproliferative Effect of Kohlrabi and Green Cabbage on Colorectal Cancer Cell Lines In Vitro.http://libraries.lau.edu.lb/research/laur/terms-of-use/articles.phphttps://www.fasebj.org/doi/abs/10.1096/fasebj.27.1_supplement.639.13enThe FASEB Journalinfo:eu-repo/semantics/openAccessoai:laur.lau.edu.lb:10725/80822021-03-19T10:43:08Z |
| spellingShingle | A comparative study of the antiproliferative effect of kohlrabi and green cabbage on colorectal cancer cell lines in vitro Rizk, Sandra |
| status_str | publishedVersion |
| title | A comparative study of the antiproliferative effect of kohlrabi and green cabbage on colorectal cancer cell lines in vitro |
| title_full | A comparative study of the antiproliferative effect of kohlrabi and green cabbage on colorectal cancer cell lines in vitro |
| title_fullStr | A comparative study of the antiproliferative effect of kohlrabi and green cabbage on colorectal cancer cell lines in vitro |
| title_full_unstemmed | A comparative study of the antiproliferative effect of kohlrabi and green cabbage on colorectal cancer cell lines in vitro |
| title_short | A comparative study of the antiproliferative effect of kohlrabi and green cabbage on colorectal cancer cell lines in vitro |
| title_sort | A comparative study of the antiproliferative effect of kohlrabi and green cabbage on colorectal cancer cell lines in vitro |
| url | http://hdl.handle.net/10725/8082 http://libraries.lau.edu.lb/research/laur/terms-of-use/articles.php https://www.fasebj.org/doi/abs/10.1096/fasebj.27.1_supplement.639.13 |