Malva pseudolavatera Leaf Extract Promotes ROS Induction Leading to Apoptosis in Acute Myeloid Leukemia Cells in Vitro
Malva pseudolavatera Webb & Berthel. is a plant from the Malvaceae family that has long been included in the human diet due to its various curative effects. Many plant leaf extracts from the various species of Malva genus have been reported to possess anti-cancer properties, however, studies on...
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| Format: | masterThesis |
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2020
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| Online Access: | http://hdl.handle.net/10725/13513 https://doi.org/10.26756/th.2022.291 http://libraries.lau.edu.lb/research/laur/terms-of-use/thesis.php |
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| _version_ | 1864513490799034368 |
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| author | El Khoury, Marianne |
| author_facet | El Khoury, Marianne |
| author_role | author |
| dc.creator.none.fl_str_mv | El Khoury, Marianne |
| dc.date.none.fl_str_mv | 2020 2020-03-14 2022-04-27T06:39:04Z 2022-04-27T06:39:04Z |
| dc.identifier.none.fl_str_mv | http://hdl.handle.net/10725/13513 https://doi.org/10.26756/th.2022.291 http://libraries.lau.edu.lb/research/laur/terms-of-use/thesis.php |
| dc.language.none.fl_str_mv | en |
| dc.publisher.none.fl_str_mv | Lebanese American University |
| dc.rights.*.fl_str_mv | info:eu-repo/semantics/openAccess |
| dc.subject.none.fl_str_mv | Acute myeloid leukemia -- Treatment Apoptosis -- Molecular aspects Malvaceae Medicinal plants Lebanese American University -- Dissertations Dissertations, Academic |
| dc.title.none.fl_str_mv | Malva pseudolavatera Leaf Extract Promotes ROS Induction Leading to Apoptosis in Acute Myeloid Leukemia Cells in Vitro |
| dc.type.none.fl_str_mv | Thesis info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/masterThesis |
| description | Malva pseudolavatera Webb & Berthel. is a plant from the Malvaceae family that has long been included in the human diet due to its various curative effects. Many plant leaf extracts from the various species of Malva genus have been reported to possess anti-cancer properties, however, studies on M. pseudolavatera leaves have documented anti-inflammatory and antioxidant effects with no emphasis on their possible anti-cancer potential. The present study explores the anti-cancer properties of M. pseudolavatera leaf extract on acute myeloid leukemia (AML) cell lines in vitro and deciphers the underlying molecular mechanism. Treatment of AML cell lines with M. pseudolavatera methanolic leaf extract showed a dose- and time-dependent inhibition of proliferation and a dose-dependent increase in apoptotic hallmarks such as an increase in phosphatidylserine on the outer membrane leaflet and membrane leakage in addition to DNA fragmentation. The pro-apoptotic effect was induced by reactive oxygen species as well as an upregulation of cleaved poly(ADP-ribose) polymerase (PARP), increase in Bax/Bcl-2 ratio, and release of cytochrome-c from the mitochondria. Major compounds of the extract included methyl linolenate, phytol, γ-sitosterol, and stigmasterol as revealed by gas chromatography coupled with mass spectrometry. This study shows that M. pseudolavatera methanolic leaf extract exhibits promising anti-cancerous effects on AML cell lines. |
| eu_rights_str_mv | openAccess |
| format | masterThesis |
| id | LAURepo_2b72a2f646242272dcb516bcc88d39fd |
| language_invalid_str_mv | en |
| network_acronym_str | LAURepo |
| network_name_str | Lebanese American University repository |
| oai_identifier_str | oai:laur.lau.edu.lb:10725/13513 |
| publishDate | 2020 |
| publisher.none.fl_str_mv | Lebanese American University |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| spelling | Malva pseudolavatera Leaf Extract Promotes ROS Induction Leading to Apoptosis in Acute Myeloid Leukemia Cells in VitroEl Khoury, MarianneAcute myeloid leukemia -- TreatmentApoptosis -- Molecular aspectsMalvaceaeMedicinal plantsLebanese American University -- DissertationsDissertations, AcademicMalva pseudolavatera Webb & Berthel. is a plant from the Malvaceae family that has long been included in the human diet due to its various curative effects. Many plant leaf extracts from the various species of Malva genus have been reported to possess anti-cancer properties, however, studies on M. pseudolavatera leaves have documented anti-inflammatory and antioxidant effects with no emphasis on their possible anti-cancer potential. The present study explores the anti-cancer properties of M. pseudolavatera leaf extract on acute myeloid leukemia (AML) cell lines in vitro and deciphers the underlying molecular mechanism. Treatment of AML cell lines with M. pseudolavatera methanolic leaf extract showed a dose- and time-dependent inhibition of proliferation and a dose-dependent increase in apoptotic hallmarks such as an increase in phosphatidylserine on the outer membrane leaflet and membrane leakage in addition to DNA fragmentation. The pro-apoptotic effect was induced by reactive oxygen species as well as an upregulation of cleaved poly(ADP-ribose) polymerase (PARP), increase in Bax/Bcl-2 ratio, and release of cytochrome-c from the mitochondria. Major compounds of the extract included methyl linolenate, phytol, γ-sitosterol, and stigmasterol as revealed by gas chromatography coupled with mass spectrometry. This study shows that M. pseudolavatera methanolic leaf extract exhibits promising anti-cancerous effects on AML cell lines.1 online resource (xv, 65 leaves) : ill. (some col.)Bibliography leaf 51-65.Lebanese American University2022-04-27T06:39:04Z2022-04-27T06:39:04Z20202020-03-14Thesisinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesishttp://hdl.handle.net/10725/13513https://doi.org/10.26756/th.2022.291http://libraries.lau.edu.lb/research/laur/terms-of-use/thesis.phpeninfo:eu-repo/semantics/openAccessoai:laur.lau.edu.lb:10725/135132022-07-13T05:47:33Z |
| spellingShingle | Malva pseudolavatera Leaf Extract Promotes ROS Induction Leading to Apoptosis in Acute Myeloid Leukemia Cells in Vitro El Khoury, Marianne Acute myeloid leukemia -- Treatment Apoptosis -- Molecular aspects Malvaceae Medicinal plants Lebanese American University -- Dissertations Dissertations, Academic |
| status_str | publishedVersion |
| title | Malva pseudolavatera Leaf Extract Promotes ROS Induction Leading to Apoptosis in Acute Myeloid Leukemia Cells in Vitro |
| title_full | Malva pseudolavatera Leaf Extract Promotes ROS Induction Leading to Apoptosis in Acute Myeloid Leukemia Cells in Vitro |
| title_fullStr | Malva pseudolavatera Leaf Extract Promotes ROS Induction Leading to Apoptosis in Acute Myeloid Leukemia Cells in Vitro |
| title_full_unstemmed | Malva pseudolavatera Leaf Extract Promotes ROS Induction Leading to Apoptosis in Acute Myeloid Leukemia Cells in Vitro |
| title_short | Malva pseudolavatera Leaf Extract Promotes ROS Induction Leading to Apoptosis in Acute Myeloid Leukemia Cells in Vitro |
| title_sort | Malva pseudolavatera Leaf Extract Promotes ROS Induction Leading to Apoptosis in Acute Myeloid Leukemia Cells in Vitro |
| topic | Acute myeloid leukemia -- Treatment Apoptosis -- Molecular aspects Malvaceae Medicinal plants Lebanese American University -- Dissertations Dissertations, Academic |
| url | http://hdl.handle.net/10725/13513 https://doi.org/10.26756/th.2022.291 http://libraries.lau.edu.lb/research/laur/terms-of-use/thesis.php |