The antitumor promoting activity of 2-himachalen-7-ol in two-stage mouse skin carcinogenesis test
Cedrus libani ssp. libani (Pinaceae) is a plant used in traditional medicine for the treatment of various diseases. 2-Himachalene-7-ol, previously isolated from the plant, possesses in vitro anticancer activity [1]. The present study investigates the chemopreventive effects of 2-himachalene-7-ol on...
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| مؤلفون آخرون: | , , , , , , , , , , , , , , |
| التنسيق: | article |
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2016
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| الوصول للمادة أونلاين: | http://hdl.handle.net/10725/7420 http://dx.doi.org/10.1055/s-0036-1596978 http://libraries.lau.edu.lb/research/laur/terms-of-use/articles.php https://www.thieme-connect.com/products/ejournals/abstract/10.1055/s-0036-1596978 |
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| _version_ | 1864513482125213696 |
|---|---|
| author | Daher, CF |
| author2 | Iskandar, RJ Dib El Jalbout, NO Dwairi, VF Zgheib, MC Ibrahim, MC Saad, JMC Bakhos, PE Chelala, NF Daou, CP Lteif, CS Sawma Awad, LJ Hakim, J. Taleb, R. El Sibai, M. Mroueh, M. |
| author2_role | author author author author author author author author author author author author author author author |
| author_facet | Daher, CF Iskandar, RJ Dib El Jalbout, NO Dwairi, VF Zgheib, MC Ibrahim, MC Saad, JMC Bakhos, PE Chelala, NF Daou, CP Lteif, CS Sawma Awad, LJ Hakim, J. Taleb, R. El Sibai, M. Mroueh, M. |
| author_role | author |
| dc.creator.none.fl_str_mv | Daher, CF Iskandar, RJ Dib El Jalbout, NO Dwairi, VF Zgheib, MC Ibrahim, MC Saad, JMC Bakhos, PE Chelala, NF Daou, CP Lteif, CS Sawma Awad, LJ Hakim, J. Taleb, R. El Sibai, M. Mroueh, M. |
| dc.date.none.fl_str_mv | 2016 2018-04-19T08:01:08Z 2018-04-19T08:01:08Z 2018-04-19 |
| dc.identifier.none.fl_str_mv | 1439-0221 http://hdl.handle.net/10725/7420 http://dx.doi.org/10.1055/s-0036-1596978 Daher, C. F., Iskandar, R. J., El Jalbout, N. D., Dwairi, V. F., Zgheib, M. C., Ibrahim, P. T., ... & Lteif, C. S. (2016). The antitumor promoting activity of 2-himachalen-7-ol in two-stage mouse skin carcinogenesis test. Planta Medica, 82(S 01), P994. http://libraries.lau.edu.lb/research/laur/terms-of-use/articles.php https://www.thieme-connect.com/products/ejournals/abstract/10.1055/s-0036-1596978 |
| dc.language.none.fl_str_mv | en |
| dc.relation.none.fl_str_mv | Planta Medica |
| dc.rights.*.fl_str_mv | info:eu-repo/semantics/openAccess |
| dc.title.none.fl_str_mv | The antitumor promoting activity of 2-himachalen-7-ol in two-stage mouse skin carcinogenesis test |
| dc.type.none.fl_str_mv | Article info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article |
| description | Cedrus libani ssp. libani (Pinaceae) is a plant used in traditional medicine for the treatment of various diseases. 2-Himachalene-7-ol, previously isolated from the plant, possesses in vitro anticancer activity [1]. The present study investigates the chemopreventive effects of 2-himachalene-7-ol on 7,12-dimethyl benz(a)anthracene (DMBA)/12-O-tetradecanoyl phorobol-13-acetate (TPA) skin tumorigenesis model in mice. The stem xylem was extracted with hexane and the obtained oil was subjected to silica gel chromatography to isolate 2-himachalen-7-ol. Papilloma were initiated on shaved dorsal skin using DMBA and promoted twice weekly using TPA for 20 weeks [2]. Animal groups were treated once weekly with 2-himachalen-7-ol via different routes: gavage (50 mg/kg), intraperitoneally (IP 10, 25, or 50 mg/kg) or topically (0.2mL of 0.5, 1 or 2.5%). Tumor yield, and volume were compared with those of non-treated control and cisplatinum-treated groups (IP 2.5 mg/kg). Liver and kidney toxicity as well as body weight changes were also investigated. Data were analysed using ANOVA. At week 16 all animal groups treated with either 2-himachalen-7-ol or cisplatinum had relatively less papilloma number (0 – 52%) and volume (30 – 66%; p < 0.05). At week 20 the number of papilloma was highest in the cisplatinum and lowest in the 2-himachalen-7-ol IP 10 mg/kg groups. Inhibition of papilloma volume was maximal in the 2-himachalen-7-ol IP 25, IP 50 and gavage (48 – 52%; p < 0.01) followed by IP 10 and topical 2.5% (43%: p < 0.05) then cisplatinum (28%; NS) groups. The least gain in body weight was observed with the cisplatinum group. Liver enzymes (ALP, ALT and AST) were not affected in all treated groups; however creatinine and urea levels were significantly higher (p < 0.05) in the cisplatinum group compared to control. In conclusion, 2-himachalen-7-ol has potent antitumor activity against DMBA/TPA skin carcinogenesis with relatively lower toxicity than commonly used chemotherapeutic drugs. |
| eu_rights_str_mv | openAccess |
| format | article |
| id | LAURepo_2d6db901ee5a2b7c855d8f9eb8f721b8 |
| identifier_str_mv | 1439-0221 Daher, C. F., Iskandar, R. J., El Jalbout, N. D., Dwairi, V. F., Zgheib, M. C., Ibrahim, P. T., ... & Lteif, C. S. (2016). The antitumor promoting activity of 2-himachalen-7-ol in two-stage mouse skin carcinogenesis test. Planta Medica, 82(S 01), P994. |
| language_invalid_str_mv | en |
| network_acronym_str | LAURepo |
| network_name_str | Lebanese American University repository |
| oai_identifier_str | oai:laur.lau.edu.lb:10725/7420 |
| publishDate | 2016 |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| spelling | The antitumor promoting activity of 2-himachalen-7-ol in two-stage mouse skin carcinogenesis testDaher, CFIskandar, RJDib El Jalbout, NODwairi, VFZgheib, MCIbrahim, MCSaad, JMCBakhos, PEChelala, NFDaou, CPLteif, CSSawma Awad, LJHakim, J.Taleb, R.El Sibai, M.Mroueh, M.Cedrus libani ssp. libani (Pinaceae) is a plant used in traditional medicine for the treatment of various diseases. 2-Himachalene-7-ol, previously isolated from the plant, possesses in vitro anticancer activity [1]. The present study investigates the chemopreventive effects of 2-himachalene-7-ol on 7,12-dimethyl benz(a)anthracene (DMBA)/12-O-tetradecanoyl phorobol-13-acetate (TPA) skin tumorigenesis model in mice. The stem xylem was extracted with hexane and the obtained oil was subjected to silica gel chromatography to isolate 2-himachalen-7-ol. Papilloma were initiated on shaved dorsal skin using DMBA and promoted twice weekly using TPA for 20 weeks [2]. Animal groups were treated once weekly with 2-himachalen-7-ol via different routes: gavage (50 mg/kg), intraperitoneally (IP 10, 25, or 50 mg/kg) or topically (0.2mL of 0.5, 1 or 2.5%). Tumor yield, and volume were compared with those of non-treated control and cisplatinum-treated groups (IP 2.5 mg/kg). Liver and kidney toxicity as well as body weight changes were also investigated. Data were analysed using ANOVA. At week 16 all animal groups treated with either 2-himachalen-7-ol or cisplatinum had relatively less papilloma number (0 – 52%) and volume (30 – 66%; p < 0.05). At week 20 the number of papilloma was highest in the cisplatinum and lowest in the 2-himachalen-7-ol IP 10 mg/kg groups. Inhibition of papilloma volume was maximal in the 2-himachalen-7-ol IP 25, IP 50 and gavage (48 – 52%; p < 0.01) followed by IP 10 and topical 2.5% (43%: p < 0.05) then cisplatinum (28%; NS) groups. The least gain in body weight was observed with the cisplatinum group. Liver enzymes (ALP, ALT and AST) were not affected in all treated groups; however creatinine and urea levels were significantly higher (p < 0.05) in the cisplatinum group compared to control. In conclusion, 2-himachalen-7-ol has potent antitumor activity against DMBA/TPA skin carcinogenesis with relatively lower toxicity than commonly used chemotherapeutic drugs.PublishedN/A2018-04-19T08:01:08Z2018-04-19T08:01:08Z20162018-04-19Articleinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1439-0221http://hdl.handle.net/10725/7420http://dx.doi.org/10.1055/s-0036-1596978Daher, C. F., Iskandar, R. J., El Jalbout, N. D., Dwairi, V. F., Zgheib, M. C., Ibrahim, P. T., ... & Lteif, C. S. (2016). The antitumor promoting activity of 2-himachalen-7-ol in two-stage mouse skin carcinogenesis test. Planta Medica, 82(S 01), P994.http://libraries.lau.edu.lb/research/laur/terms-of-use/articles.phphttps://www.thieme-connect.com/products/ejournals/abstract/10.1055/s-0036-1596978enPlanta Medicainfo:eu-repo/semantics/openAccessoai:laur.lau.edu.lb:10725/74202024-01-29T13:44:23Z |
| spellingShingle | The antitumor promoting activity of 2-himachalen-7-ol in two-stage mouse skin carcinogenesis test Daher, CF |
| status_str | publishedVersion |
| title | The antitumor promoting activity of 2-himachalen-7-ol in two-stage mouse skin carcinogenesis test |
| title_full | The antitumor promoting activity of 2-himachalen-7-ol in two-stage mouse skin carcinogenesis test |
| title_fullStr | The antitumor promoting activity of 2-himachalen-7-ol in two-stage mouse skin carcinogenesis test |
| title_full_unstemmed | The antitumor promoting activity of 2-himachalen-7-ol in two-stage mouse skin carcinogenesis test |
| title_short | The antitumor promoting activity of 2-himachalen-7-ol in two-stage mouse skin carcinogenesis test |
| title_sort | The antitumor promoting activity of 2-himachalen-7-ol in two-stage mouse skin carcinogenesis test |
| url | http://hdl.handle.net/10725/7420 http://dx.doi.org/10.1055/s-0036-1596978 http://libraries.lau.edu.lb/research/laur/terms-of-use/articles.php https://www.thieme-connect.com/products/ejournals/abstract/10.1055/s-0036-1596978 |