Monitoring the progression of the in vitro selection of nucleic acid aptamers by denaturing high-performance liquid chromatography
Monitoring of in-vitro selection experiments is crucial in evaluation of the success and outcome of such approaches. Furthermore, monitoring running in parallel with the selection procedure enables early intervention and adjustment of stringency to achieve the desired activities of the selected nucl...
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2008
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| Online Access: | http://hdl.handle.net/10725/6181 http://dx.doi.org/10.1007/s00216-007-1699-8 http://libraries.lau.edu.lb/research/laur/terms-of-use/articles.php https://link.springer.com/article/10.1007/s00216-007-1699-8 |
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| _version_ | 1864513478863093760 |
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| author | El Maarri, Osman |
| author2 | Muller, Jens Oldenburg, Johannes Potzsch, Bernd |
| author2_role | author author author |
| author_facet | El Maarri, Osman Muller, Jens Oldenburg, Johannes Potzsch, Bernd |
| author_role | author |
| dc.creator.none.fl_str_mv | El Maarri, Osman Muller, Jens Oldenburg, Johannes Potzsch, Bernd |
| dc.date.none.fl_str_mv | 2008 2017-09-13T11:16:09Z 2017-09-13T11:16:09Z 2017-09-13 |
| dc.identifier.none.fl_str_mv | 1618-2650 http://hdl.handle.net/10725/6181 http://dx.doi.org/10.1007/s00216-007-1699-8 Müller, J., El-Maarri, O., Oldenburg, J., Pötzsch, B., & Mayer, G. (2008). Monitoring the progression of the in vitro selection of nucleic acid aptamers by denaturing high-performance liquid chromatography. Analytical and bioanalytical chemistry, 390(4), 1033-1037. http://libraries.lau.edu.lb/research/laur/terms-of-use/articles.php https://link.springer.com/article/10.1007/s00216-007-1699-8 |
| dc.language.none.fl_str_mv | en |
| dc.relation.none.fl_str_mv | Analytical and Bioanalytical Chemistry |
| dc.rights.*.fl_str_mv | info:eu-repo/semantics/openAccess |
| dc.title.none.fl_str_mv | Monitoring the progression of the in vitro selection of nucleic acid aptamers by denaturing high-performance liquid chromatography |
| dc.type.none.fl_str_mv | Article info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article |
| description | Monitoring of in-vitro selection experiments is crucial in evaluation of the success and outcome of such approaches. Furthermore, monitoring running in parallel with the selection procedure enables early intervention and adjustment of stringency to achieve the desired activities of the selected nucleic acid species. Here we describe the use of a non-radioactive method that enables monitoring of a SELEX procedure on the basis of sequence diversity. We employ denaturing HPLC and describe for the first time an experimental set-up that is useful both for analysis of the progression of in-vitro selection experiments and for separation of distinct aptamer sequences. |
| eu_rights_str_mv | openAccess |
| format | article |
| id | LAURepo_2d97bf395ebfb09422279ed8b85a02b9 |
| identifier_str_mv | 1618-2650 Müller, J., El-Maarri, O., Oldenburg, J., Pötzsch, B., & Mayer, G. (2008). Monitoring the progression of the in vitro selection of nucleic acid aptamers by denaturing high-performance liquid chromatography. Analytical and bioanalytical chemistry, 390(4), 1033-1037. |
| language_invalid_str_mv | en |
| network_acronym_str | LAURepo |
| network_name_str | Lebanese American University repository |
| oai_identifier_str | oai:laur.lau.edu.lb:10725/6181 |
| publishDate | 2008 |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| spelling | Monitoring the progression of the in vitro selection of nucleic acid aptamers by denaturing high-performance liquid chromatographyEl Maarri, OsmanMuller, JensOldenburg, JohannesPotzsch, BerndMonitoring of in-vitro selection experiments is crucial in evaluation of the success and outcome of such approaches. Furthermore, monitoring running in parallel with the selection procedure enables early intervention and adjustment of stringency to achieve the desired activities of the selected nucleic acid species. Here we describe the use of a non-radioactive method that enables monitoring of a SELEX procedure on the basis of sequence diversity. We employ denaturing HPLC and describe for the first time an experimental set-up that is useful both for analysis of the progression of in-vitro selection experiments and for separation of distinct aptamer sequences.PublishedN/A2017-09-13T11:16:09Z2017-09-13T11:16:09Z20082017-09-13Articleinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1618-2650http://hdl.handle.net/10725/6181http://dx.doi.org/10.1007/s00216-007-1699-8Müller, J., El-Maarri, O., Oldenburg, J., Pötzsch, B., & Mayer, G. (2008). Monitoring the progression of the in vitro selection of nucleic acid aptamers by denaturing high-performance liquid chromatography. Analytical and bioanalytical chemistry, 390(4), 1033-1037.http://libraries.lau.edu.lb/research/laur/terms-of-use/articles.phphttps://link.springer.com/article/10.1007/s00216-007-1699-8enAnalytical and Bioanalytical Chemistryinfo:eu-repo/semantics/openAccessoai:laur.lau.edu.lb:10725/61812021-03-19T10:00:49Z |
| spellingShingle | Monitoring the progression of the in vitro selection of nucleic acid aptamers by denaturing high-performance liquid chromatography El Maarri, Osman |
| status_str | publishedVersion |
| title | Monitoring the progression of the in vitro selection of nucleic acid aptamers by denaturing high-performance liquid chromatography |
| title_full | Monitoring the progression of the in vitro selection of nucleic acid aptamers by denaturing high-performance liquid chromatography |
| title_fullStr | Monitoring the progression of the in vitro selection of nucleic acid aptamers by denaturing high-performance liquid chromatography |
| title_full_unstemmed | Monitoring the progression of the in vitro selection of nucleic acid aptamers by denaturing high-performance liquid chromatography |
| title_short | Monitoring the progression of the in vitro selection of nucleic acid aptamers by denaturing high-performance liquid chromatography |
| title_sort | Monitoring the progression of the in vitro selection of nucleic acid aptamers by denaturing high-performance liquid chromatography |
| url | http://hdl.handle.net/10725/6181 http://dx.doi.org/10.1007/s00216-007-1699-8 http://libraries.lau.edu.lb/research/laur/terms-of-use/articles.php https://link.springer.com/article/10.1007/s00216-007-1699-8 |