Antibiotic resistance patterns and sequencing of class I integron from uropathogenic Escherichia coli in Lebanon
Aim: To study the prevalence and molecular basis of antimicrobial resistance in UPEC. Methods and Results: PCR was used to detect the presence of the Class I integron variable region (VR). The VR amplicons were then characterized by partial sequencing and restriction digestion with AluI. VR negati...
محفوظ في:
| المؤلف الرئيسي: | |
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| مؤلفون آخرون: | , , |
| التنسيق: | article |
| منشور في: |
2010
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| الوصول للمادة أونلاين: | http://hdl.handle.net/10725/3478 http://dx.doi.org/10.1111/j.1472-765X.2010.02926.x http://onlinelibrary.wiley.com/doi/10.1111/j.1472-765X.2010.02926.x/full |
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| الملخص: | Aim: To study the prevalence and molecular basis of antimicrobial resistance in UPEC. Methods and Results: PCR was used to detect the presence of the Class I integron variable region (VR). The VR amplicons were then characterized by partial sequencing and restriction digestion with AluI. VR negative isolates showed more antibiotic susceptibility than VR positive isolates. 30% of the isolates were positive for the VR and carried the genes dfrA7, dfrA17-aadA5, dfrA1-aadA1, dfrA12-orf5-aadA2 and blaOXA-30-aadA1. Five restriction patterns were detected and isolates with the same VR amplicon size had the same restriction pattern. Conclusions: Our data demonstrated that Class I integrons are widely disseminated in Lebanon and showed their importance for the occurrence and transmission of multidrug resistance. Significance and Impact of the Study: These findings will facilitate greater understanding of the factors that contribute to the presence and transfer of integron-associated antibiotic resistance genes in UPEC. |
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