Antibiotic resistance patterns and sequencing of class I integron from uropathogenic Escherichia coli in Lebanon
Aim: To study the prevalence and molecular basis of antimicrobial resistance in UPEC. Methods and Results: PCR was used to detect the presence of the Class I integron variable region (VR). The VR amplicons were then characterized by partial sequencing and restriction digestion with AluI. VR negati...
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2010
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| Online Access: | http://hdl.handle.net/10725/3478 http://dx.doi.org/10.1111/j.1472-765X.2010.02926.x http://onlinelibrary.wiley.com/doi/10.1111/j.1472-765X.2010.02926.x/full |
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| _version_ | 1864513461303640064 |
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| author | Hashwa, F. A. |
| author2 | Tokajian, S. T. Farah, M. J. El-Najjar, N. G. |
| author2_role | author author author |
| author_facet | Hashwa, F. A. Tokajian, S. T. Farah, M. J. El-Najjar, N. G. |
| author_role | author |
| dc.creator.none.fl_str_mv | Hashwa, F. A. Tokajian, S. T. Farah, M. J. El-Najjar, N. G. |
| dc.date.none.fl_str_mv | 2010 2016-04-01T11:00:25Z 2016-04-01T11:00:25Z 2016-04-01 |
| dc.identifier.none.fl_str_mv | 0266-8254 http://hdl.handle.net/10725/3478 http://dx.doi.org/10.1111/j.1472-765X.2010.02926.x El‐Najjar, N. G., Farah, M. J., Hashwa, F. A., & Tokajian, S. T. (2010). Antibiotic resistance patterns and sequencing of class I integron from uropathogenic Escherichia coli in Lebanon. Letters in applied microbiology, 51(4), 456-461. http://onlinelibrary.wiley.com/doi/10.1111/j.1472-765X.2010.02926.x/full |
| dc.language.none.fl_str_mv | en |
| dc.relation.none.fl_str_mv | Letters in Applied Microbiology |
| dc.rights.*.fl_str_mv | info:eu-repo/semantics/openAccess |
| dc.title.none.fl_str_mv | Antibiotic resistance patterns and sequencing of class I integron from uropathogenic Escherichia coli in Lebanon |
| dc.type.none.fl_str_mv | Article info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article |
| description | Aim: To study the prevalence and molecular basis of antimicrobial resistance in UPEC. Methods and Results: PCR was used to detect the presence of the Class I integron variable region (VR). The VR amplicons were then characterized by partial sequencing and restriction digestion with AluI. VR negative isolates showed more antibiotic susceptibility than VR positive isolates. 30% of the isolates were positive for the VR and carried the genes dfrA7, dfrA17-aadA5, dfrA1-aadA1, dfrA12-orf5-aadA2 and blaOXA-30-aadA1. Five restriction patterns were detected and isolates with the same VR amplicon size had the same restriction pattern. Conclusions: Our data demonstrated that Class I integrons are widely disseminated in Lebanon and showed their importance for the occurrence and transmission of multidrug resistance. Significance and Impact of the Study: These findings will facilitate greater understanding of the factors that contribute to the presence and transfer of integron-associated antibiotic resistance genes in UPEC. |
| eu_rights_str_mv | openAccess |
| format | article |
| id | LAURepo_2f86ac504ac63bd86efa2d73df3efd80 |
| identifier_str_mv | 0266-8254 El‐Najjar, N. G., Farah, M. J., Hashwa, F. A., & Tokajian, S. T. (2010). Antibiotic resistance patterns and sequencing of class I integron from uropathogenic Escherichia coli in Lebanon. Letters in applied microbiology, 51(4), 456-461. |
| language_invalid_str_mv | en |
| network_acronym_str | LAURepo |
| network_name_str | Lebanese American University repository |
| oai_identifier_str | oai:laur.lau.edu.lb:10725/3478 |
| publishDate | 2010 |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| spelling | Antibiotic resistance patterns and sequencing of class I integron from uropathogenic Escherichia coli in LebanonHashwa, F. A.Tokajian, S. T.Farah, M. J.El-Najjar, N. G.Aim: To study the prevalence and molecular basis of antimicrobial resistance in UPEC. Methods and Results: PCR was used to detect the presence of the Class I integron variable region (VR). The VR amplicons were then characterized by partial sequencing and restriction digestion with AluI. VR negative isolates showed more antibiotic susceptibility than VR positive isolates. 30% of the isolates were positive for the VR and carried the genes dfrA7, dfrA17-aadA5, dfrA1-aadA1, dfrA12-orf5-aadA2 and blaOXA-30-aadA1. Five restriction patterns were detected and isolates with the same VR amplicon size had the same restriction pattern. Conclusions: Our data demonstrated that Class I integrons are widely disseminated in Lebanon and showed their importance for the occurrence and transmission of multidrug resistance. Significance and Impact of the Study: These findings will facilitate greater understanding of the factors that contribute to the presence and transfer of integron-associated antibiotic resistance genes in UPEC.PublishedN/A2016-04-01T11:00:25Z2016-04-01T11:00:25Z20102016-04-01Articleinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article0266-8254http://hdl.handle.net/10725/3478http://dx.doi.org/10.1111/j.1472-765X.2010.02926.xEl‐Najjar, N. G., Farah, M. J., Hashwa, F. A., & Tokajian, S. T. (2010). Antibiotic resistance patterns and sequencing of class I integron from uropathogenic Escherichia coli in Lebanon. Letters in applied microbiology, 51(4), 456-461.http://onlinelibrary.wiley.com/doi/10.1111/j.1472-765X.2010.02926.x/fullenLetters in Applied Microbiologyinfo:eu-repo/semantics/openAccessoai:laur.lau.edu.lb:10725/34782019-02-19T14:00:58Z |
| spellingShingle | Antibiotic resistance patterns and sequencing of class I integron from uropathogenic Escherichia coli in Lebanon Hashwa, F. A. |
| status_str | publishedVersion |
| title | Antibiotic resistance patterns and sequencing of class I integron from uropathogenic Escherichia coli in Lebanon |
| title_full | Antibiotic resistance patterns and sequencing of class I integron from uropathogenic Escherichia coli in Lebanon |
| title_fullStr | Antibiotic resistance patterns and sequencing of class I integron from uropathogenic Escherichia coli in Lebanon |
| title_full_unstemmed | Antibiotic resistance patterns and sequencing of class I integron from uropathogenic Escherichia coli in Lebanon |
| title_short | Antibiotic resistance patterns and sequencing of class I integron from uropathogenic Escherichia coli in Lebanon |
| title_sort | Antibiotic resistance patterns and sequencing of class I integron from uropathogenic Escherichia coli in Lebanon |
| url | http://hdl.handle.net/10725/3478 http://dx.doi.org/10.1111/j.1472-765X.2010.02926.x http://onlinelibrary.wiley.com/doi/10.1111/j.1472-765X.2010.02926.x/full |