Changes in sphingomyelinases, ceramide, Bax, Bcl2, and caspase-3 during and after experimental status epilepticus
Status epilepticus (SE) induces a number of events leading to programmed cell death (PCD). The aim of our work is to study the time sequence of activation of different factors in experimental SE (intraperitoneal kainic acid (KA) model). We studied ceramide, a known mediator of apoptosis in multiple...
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| المؤلف الرئيسي: | |
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| مؤلفون آخرون: | , , , , , , |
| التنسيق: | article |
| منشور في: |
2008
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| الوصول للمادة أونلاين: | http://hdl.handle.net/10725/2733 http://dx.doi.org/10.1016/j.eplepsyres.2008.05.009 http://www.sciencedirect.com/science/article/pii/S0920121108001393 |
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إضافة وسم
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| _version_ | 1864513459120504832 |
|---|---|
| author | Abi-Habib, Ralph |
| author2 | Mikati, Mohamad A. Zeineh, Michel El Hokayem, Jimmy Rahmeh, Amal El Sabban, Marwan Usta, Julnar Dbaibo, Ghassan |
| author2_role | author author author author author author author |
| author_facet | Abi-Habib, Ralph Mikati, Mohamad A. Zeineh, Michel El Hokayem, Jimmy Rahmeh, Amal El Sabban, Marwan Usta, Julnar Dbaibo, Ghassan |
| author_role | author |
| dc.creator.none.fl_str_mv | Abi-Habib, Ralph Mikati, Mohamad A. Zeineh, Michel El Hokayem, Jimmy Rahmeh, Amal El Sabban, Marwan Usta, Julnar Dbaibo, Ghassan |
| dc.date.none.fl_str_mv | 2008 2015-11-30T09:08:50Z 2015-11-30T09:08:50Z 2015-11-30 |
| dc.identifier.none.fl_str_mv | 0920-1211 http://hdl.handle.net/10725/2733 http://dx.doi.org/10.1016/j.eplepsyres.2008.05.009 Mikati, M. A., Zeinieh, M., Habib, R. A., El Hokayem, J., Rahmeh, A., El Sabban, M., ... & Dbaibo, G. (2008). Changes in sphingomyelinases, ceramide, Bax, Bcl 2, and caspase-3 during and after experimental status epilepticus. Epilepsy research, 81(2), 161-166. http://www.sciencedirect.com/science/article/pii/S0920121108001393 |
| dc.language.none.fl_str_mv | en |
| dc.relation.none.fl_str_mv | Epilepsy Research |
| dc.rights.*.fl_str_mv | info:eu-repo/semantics/openAccess |
| dc.title.none.fl_str_mv | Changes in sphingomyelinases, ceramide, Bax, Bcl2, and caspase-3 during and after experimental status epilepticus |
| dc.type.none.fl_str_mv | Article info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article |
| description | Status epilepticus (SE) induces a number of events leading to programmed cell death (PCD). The aim of our work is to study the time sequence of activation of different factors in experimental SE (intraperitoneal kainic acid (KA) model). We studied ceramide, a known mediator of apoptosis in multiple models, sphingomyelinases (SMases), enzymes that break down sphingomyelin and increase ceramide thus leading to apoptosis in many models, Bcl2, Bax, and caspase-3. SE induced a sustained ceramide increase starting 2 h after kainic acid injection followed by an increase in Bax protein at 6 and 12 h, and the appearance of caspase-3-activated fragment (caspase-3a) immunostaining and TUNEL positivity at 12 h. Status epilepticus also induced an increase in acidic and neutral sphingomyelinases that preceded (acidic sphingomyelinase) and parallelled (acidic and neutral sphingomyelinase) the increases in ceramide. These data suggest that, in this model, Bax is activated early in the process and that its increase is sustained till 12 h after kainic acid injection which is the time of first appearance of caspase-3 activation and TUNEL positivity, and that SMases contribute to increases in ceramide levels during and after status epilepticus. |
| eu_rights_str_mv | openAccess |
| format | article |
| id | LAURepo_31cc8d702b303f48d12decdfadf9e994 |
| identifier_str_mv | 0920-1211 Mikati, M. A., Zeinieh, M., Habib, R. A., El Hokayem, J., Rahmeh, A., El Sabban, M., ... & Dbaibo, G. (2008). Changes in sphingomyelinases, ceramide, Bax, Bcl 2, and caspase-3 during and after experimental status epilepticus. Epilepsy research, 81(2), 161-166. |
| language_invalid_str_mv | en |
| network_acronym_str | LAURepo |
| network_name_str | Lebanese American University repository |
| oai_identifier_str | oai:laur.lau.edu.lb:10725/2733 |
| publishDate | 2008 |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| spelling | Changes in sphingomyelinases, ceramide, Bax, Bcl2, and caspase-3 during and after experimental status epilepticusAbi-Habib, RalphMikati, Mohamad A.Zeineh, MichelEl Hokayem, JimmyRahmeh, AmalEl Sabban, MarwanUsta, JulnarDbaibo, GhassanStatus epilepticus (SE) induces a number of events leading to programmed cell death (PCD). The aim of our work is to study the time sequence of activation of different factors in experimental SE (intraperitoneal kainic acid (KA) model). We studied ceramide, a known mediator of apoptosis in multiple models, sphingomyelinases (SMases), enzymes that break down sphingomyelin and increase ceramide thus leading to apoptosis in many models, Bcl2, Bax, and caspase-3. SE induced a sustained ceramide increase starting 2 h after kainic acid injection followed by an increase in Bax protein at 6 and 12 h, and the appearance of caspase-3-activated fragment (caspase-3a) immunostaining and TUNEL positivity at 12 h. Status epilepticus also induced an increase in acidic and neutral sphingomyelinases that preceded (acidic sphingomyelinase) and parallelled (acidic and neutral sphingomyelinase) the increases in ceramide. These data suggest that, in this model, Bax is activated early in the process and that its increase is sustained till 12 h after kainic acid injection which is the time of first appearance of caspase-3 activation and TUNEL positivity, and that SMases contribute to increases in ceramide levels during and after status epilepticus.PublishedN/A2015-11-30T09:08:50Z2015-11-30T09:08:50Z20082015-11-30Articleinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article0920-1211http://hdl.handle.net/10725/2733http://dx.doi.org/10.1016/j.eplepsyres.2008.05.009Mikati, M. A., Zeinieh, M., Habib, R. A., El Hokayem, J., Rahmeh, A., El Sabban, M., ... & Dbaibo, G. (2008). Changes in sphingomyelinases, ceramide, Bax, Bcl 2, and caspase-3 during and after experimental status epilepticus. Epilepsy research, 81(2), 161-166.http://www.sciencedirect.com/science/article/pii/S0920121108001393enEpilepsy Researchinfo:eu-repo/semantics/openAccessoai:laur.lau.edu.lb:10725/27332016-08-01T05:59:42Z |
| spellingShingle | Changes in sphingomyelinases, ceramide, Bax, Bcl2, and caspase-3 during and after experimental status epilepticus Abi-Habib, Ralph |
| status_str | publishedVersion |
| title | Changes in sphingomyelinases, ceramide, Bax, Bcl2, and caspase-3 during and after experimental status epilepticus |
| title_full | Changes in sphingomyelinases, ceramide, Bax, Bcl2, and caspase-3 during and after experimental status epilepticus |
| title_fullStr | Changes in sphingomyelinases, ceramide, Bax, Bcl2, and caspase-3 during and after experimental status epilepticus |
| title_full_unstemmed | Changes in sphingomyelinases, ceramide, Bax, Bcl2, and caspase-3 during and after experimental status epilepticus |
| title_short | Changes in sphingomyelinases, ceramide, Bax, Bcl2, and caspase-3 during and after experimental status epilepticus |
| title_sort | Changes in sphingomyelinases, ceramide, Bax, Bcl2, and caspase-3 during and after experimental status epilepticus |
| url | http://hdl.handle.net/10725/2733 http://dx.doi.org/10.1016/j.eplepsyres.2008.05.009 http://www.sciencedirect.com/science/article/pii/S0920121108001393 |