DNA methylation at promoter regions regulates the timing of gene activation in xenopus laevis embryos
The levels of genomic DNA methylation in vertebrate species display a wide range of developmental dynamics. Here, we show that in contrast to mice, the paternal genome of the amphibian, Xenopus laevis, is not subjected to active demethylation of 5-methyl cytosine immediately after fertilization. Hig...
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2002
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| Online Access: | http://hdl.handle.net/10725/6166 https://doi.org/10.1006/dbio.2001.0560 http://libraries.lau.edu.lb/research/laur/terms-of-use/articles.php http://www.sciencedirect.com/science/article/pii/S0012160601905603 |
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| _version_ | 1864513478620872704 |
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| author | El Maarri, Osman |
| author2 | Stancheva, Irina Walter, Joern Niveleau, Alain Meehan, Richard R. |
| author2_role | author author author author |
| author_facet | El Maarri, Osman Stancheva, Irina Walter, Joern Niveleau, Alain Meehan, Richard R. |
| author_role | author |
| dc.creator.none.fl_str_mv | El Maarri, Osman Stancheva, Irina Walter, Joern Niveleau, Alain Meehan, Richard R. |
| dc.date.none.fl_str_mv | 2002 2017-09-12T11:09:51Z 2017-09-12T11:09:51Z 2017-09-12 |
| dc.identifier.none.fl_str_mv | 1095-564X http://hdl.handle.net/10725/6166 https://doi.org/10.1006/dbio.2001.0560 Stancheva, I., El-Maarri, O., Walter, J., Niveleau, A., & Meehan, R. R. (2002). DNA methylation at promoter regions regulates the timing of gene activation in Xenopus laevis embryos. Developmental biology, 243(1), 155-165. http://libraries.lau.edu.lb/research/laur/terms-of-use/articles.php http://www.sciencedirect.com/science/article/pii/S0012160601905603 |
| dc.language.none.fl_str_mv | en |
| dc.relation.none.fl_str_mv | Developmental Biology |
| dc.rights.*.fl_str_mv | info:eu-repo/semantics/openAccess |
| dc.title.none.fl_str_mv | DNA methylation at promoter regions regulates the timing of gene activation in xenopus laevis embryos |
| dc.type.none.fl_str_mv | Article info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article |
| description | The levels of genomic DNA methylation in vertebrate species display a wide range of developmental dynamics. Here, we show that in contrast to mice, the paternal genome of the amphibian, Xenopus laevis, is not subjected to active demethylation of 5-methyl cytosine immediately after fertilization. High levels of methylation in the DNA of both oocyte and sperm are maintained in the early embryo but progressively decline during the cleavage stages. As a result, the Xenopus genome has its lowest methylation content at the midblastula transition (MBT) and during subsequent gastrulation. Between blastula and gastrula stages, we detect a loss of methylation at individual Xenopus gene promoters (TFIIIA, Xbra, and c-Myc II) that are activated at MBT. No changes are observed in the methylation patterns of repeated sequences, genes that are inactive at MBT, or in the coding regions of individual genes. In embryos that are depleted of the maintenance methyltransferase enzyme (xDnmt1), these developmentally programmed changes in promoter methylation are disrupted, which may account for the altered patterns of gene expression that occur in these embryos. Our results suggest that DNA methylation has a role in regulating the timing of gene activation at MBT in Xenopus laevis embryos. |
| eu_rights_str_mv | openAccess |
| format | article |
| id | LAURepo_3307a7b10f63eda0a0dc8aaee8624b9f |
| identifier_str_mv | 1095-564X Stancheva, I., El-Maarri, O., Walter, J., Niveleau, A., & Meehan, R. R. (2002). DNA methylation at promoter regions regulates the timing of gene activation in Xenopus laevis embryos. Developmental biology, 243(1), 155-165. |
| language_invalid_str_mv | en |
| network_acronym_str | LAURepo |
| network_name_str | Lebanese American University repository |
| oai_identifier_str | oai:laur.lau.edu.lb:10725/6166 |
| publishDate | 2002 |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| spelling | DNA methylation at promoter regions regulates the timing of gene activation in xenopus laevis embryosEl Maarri, OsmanStancheva, IrinaWalter, JoernNiveleau, AlainMeehan, Richard R.The levels of genomic DNA methylation in vertebrate species display a wide range of developmental dynamics. Here, we show that in contrast to mice, the paternal genome of the amphibian, Xenopus laevis, is not subjected to active demethylation of 5-methyl cytosine immediately after fertilization. High levels of methylation in the DNA of both oocyte and sperm are maintained in the early embryo but progressively decline during the cleavage stages. As a result, the Xenopus genome has its lowest methylation content at the midblastula transition (MBT) and during subsequent gastrulation. Between blastula and gastrula stages, we detect a loss of methylation at individual Xenopus gene promoters (TFIIIA, Xbra, and c-Myc II) that are activated at MBT. No changes are observed in the methylation patterns of repeated sequences, genes that are inactive at MBT, or in the coding regions of individual genes. In embryos that are depleted of the maintenance methyltransferase enzyme (xDnmt1), these developmentally programmed changes in promoter methylation are disrupted, which may account for the altered patterns of gene expression that occur in these embryos. Our results suggest that DNA methylation has a role in regulating the timing of gene activation at MBT in Xenopus laevis embryos.PublishedN/A2017-09-12T11:09:51Z2017-09-12T11:09:51Z20022017-09-12Articleinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1095-564Xhttp://hdl.handle.net/10725/6166https://doi.org/10.1006/dbio.2001.0560Stancheva, I., El-Maarri, O., Walter, J., Niveleau, A., & Meehan, R. R. (2002). DNA methylation at promoter regions regulates the timing of gene activation in Xenopus laevis embryos. Developmental biology, 243(1), 155-165.http://libraries.lau.edu.lb/research/laur/terms-of-use/articles.phphttp://www.sciencedirect.com/science/article/pii/S0012160601905603enDevelopmental Biologyinfo:eu-repo/semantics/openAccessoai:laur.lau.edu.lb:10725/61662021-03-19T10:00:49Z |
| spellingShingle | DNA methylation at promoter regions regulates the timing of gene activation in xenopus laevis embryos El Maarri, Osman |
| status_str | publishedVersion |
| title | DNA methylation at promoter regions regulates the timing of gene activation in xenopus laevis embryos |
| title_full | DNA methylation at promoter regions regulates the timing of gene activation in xenopus laevis embryos |
| title_fullStr | DNA methylation at promoter regions regulates the timing of gene activation in xenopus laevis embryos |
| title_full_unstemmed | DNA methylation at promoter regions regulates the timing of gene activation in xenopus laevis embryos |
| title_short | DNA methylation at promoter regions regulates the timing of gene activation in xenopus laevis embryos |
| title_sort | DNA methylation at promoter regions regulates the timing of gene activation in xenopus laevis embryos |
| url | http://hdl.handle.net/10725/6166 https://doi.org/10.1006/dbio.2001.0560 http://libraries.lau.edu.lb/research/laur/terms-of-use/articles.php http://www.sciencedirect.com/science/article/pii/S0012160601905603 |