Gasoline fume inhalation induces apoptosis, inflammation, and favors Th2 polarization in C57BL/6 mice
Gasoline exposure has been widely reported in the literature as being toxic to human health. However, the exact underlying molecular mechanisms triggered by its inhalation have not been thoroughly investigated. We herein present a model of sub-chronic, static gasoline vapor inhalation in adult femal...
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2022
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| Online Access: | http://hdl.handle.net/10725/14354 https://doi.org/10.1002/jat.4286 http://libraries.lau.edu.lb/research/laur/terms-of-use/articles.php https://analyticalsciencejournals.onlinelibrary.wiley.com/doi/full/10.1002/jat.4286 |
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| _version_ | 1864513469263380480 |
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| author | Nour-Eldine, Wared |
| author2 | Sayyed, Katia Harhous, Zeina Dagher-Hamalian, Carole Mehanna, Stephanie Achkouti, Donna ElKazzaz, Hanan Khnayzer, Rony S. Kobeissy, Firas Khalil, Christian Abi-Gerges, Aniella |
| author2_role | author author author author author author author author author author |
| author_facet | Nour-Eldine, Wared Sayyed, Katia Harhous, Zeina Dagher-Hamalian, Carole Mehanna, Stephanie Achkouti, Donna ElKazzaz, Hanan Khnayzer, Rony S. Kobeissy, Firas Khalil, Christian Abi-Gerges, Aniella |
| author_role | author |
| dc.creator.none.fl_str_mv | Nour-Eldine, Wared Sayyed, Katia Harhous, Zeina Dagher-Hamalian, Carole Mehanna, Stephanie Achkouti, Donna ElKazzaz, Hanan Khnayzer, Rony S. Kobeissy, Firas Khalil, Christian Abi-Gerges, Aniella |
| dc.date.none.fl_str_mv | 2022 2023-01-09T13:44:35Z 2023-01-09T13:44:35Z 2023-01-09 |
| dc.identifier.none.fl_str_mv | 0260-437X http://hdl.handle.net/10725/14354 https://doi.org/10.1002/jat.4286 Nour‐Eldine, W., Sayyed, K., Harhous, Z., Dagher‐Hamalian, C., Mehanna, S., Achkouti, D., ... & Abi‐Gerges, A. (2022). Gasoline fume inhalation induces apoptosis, inflammation, and favors Th2 polarization in C57BL/6 mice. Journal of Applied Toxicology, 42(7), 1178-1191. http://libraries.lau.edu.lb/research/laur/terms-of-use/articles.php https://analyticalsciencejournals.onlinelibrary.wiley.com/doi/full/10.1002/jat.4286 |
| dc.language.none.fl_str_mv | en |
| dc.relation.none.fl_str_mv | Journal of Applied Toxicology |
| dc.rights.*.fl_str_mv | info:eu-repo/semantics/openAccess |
| dc.title.none.fl_str_mv | Gasoline fume inhalation induces apoptosis, inflammation, and favors Th2 polarization in C57BL/6 mice |
| dc.type.none.fl_str_mv | Article info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article |
| description | Gasoline exposure has been widely reported in the literature as being toxic to human health. However, the exact underlying molecular mechanisms triggered by its inhalation have not been thoroughly investigated. We herein present a model of sub-chronic, static gasoline vapor inhalation in adult female C57BL/6 mice. Animals were exposed daily to either gasoline vapors (0.86 g/animal/90 min) or ambient air for 5 days/week over 7 consecutive weeks. At the end of the study period, toxic and molecular mechanisms underlying the inflammatory, oxidative, and apoptotic effects triggered by gasoline vapors, were examined in the lungs and liver of gasoline-exposed (GE) mice. Static gasoline exposure induced a significant increase (+21%) in lungs/body weight (BW) ratio in GE versus control (CON) mice along with a pulmonary inflammation attested by histological staining. The latter was consistent with increases in the transcript levels of proinflammatory cytokines [Interleukins (ILs) 4 and 6], respectively by ~ 6- and 4-fold in the lungs of GE mice compared to CON. Interestingly, IL-10 expression was also increased by ~ 10-fold in the lungs of GE mice suggesting an attempt to counterbalance the established inflammation. Moreover, the pulmonary expression of IL-12 and TNF-α was downregulated by 2- and 4-fold, respectively, suggesting the skewing toward Th2 phenotype. Additionally, GE mice showed a significant upregulation in Bax/Bcl-2 ratio, caspases 3, 8, and 9 with no change in JNK expression in the lungs, suggesting the activation of both intrinsic and extrinsic apoptotic pathways. Static gasoline exposure over seven consecutive weeks had a minor hepatic portal inflammation attested by H&E staining along with an increase in the hepatic expression of the mitochondrial complexes in GE mice. Therefore, tissue damage biomarkers highlight the health risks associated with vapor exposure and may present potential therapeutic targets for recovery from gasoline intoxication. |
| eu_rights_str_mv | openAccess |
| format | article |
| id | LAURepo_3c286c77cab4459bc42676ce9cfd6bbd |
| identifier_str_mv | 0260-437X Nour‐Eldine, W., Sayyed, K., Harhous, Z., Dagher‐Hamalian, C., Mehanna, S., Achkouti, D., ... & Abi‐Gerges, A. (2022). Gasoline fume inhalation induces apoptosis, inflammation, and favors Th2 polarization in C57BL/6 mice. Journal of Applied Toxicology, 42(7), 1178-1191. |
| language_invalid_str_mv | en |
| network_acronym_str | LAURepo |
| network_name_str | Lebanese American University repository |
| oai_identifier_str | oai:laur.lau.edu.lb:10725/14354 |
| publishDate | 2022 |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| spelling | Gasoline fume inhalation induces apoptosis, inflammation, and favors Th2 polarization in C57BL/6 miceNour-Eldine, WaredSayyed, KatiaHarhous, ZeinaDagher-Hamalian, CaroleMehanna, StephanieAchkouti, DonnaElKazzaz, HananKhnayzer, Rony S.Kobeissy, FirasKhalil, ChristianAbi-Gerges, AniellaGasoline exposure has been widely reported in the literature as being toxic to human health. However, the exact underlying molecular mechanisms triggered by its inhalation have not been thoroughly investigated. We herein present a model of sub-chronic, static gasoline vapor inhalation in adult female C57BL/6 mice. Animals were exposed daily to either gasoline vapors (0.86 g/animal/90 min) or ambient air for 5 days/week over 7 consecutive weeks. At the end of the study period, toxic and molecular mechanisms underlying the inflammatory, oxidative, and apoptotic effects triggered by gasoline vapors, were examined in the lungs and liver of gasoline-exposed (GE) mice. Static gasoline exposure induced a significant increase (+21%) in lungs/body weight (BW) ratio in GE versus control (CON) mice along with a pulmonary inflammation attested by histological staining. The latter was consistent with increases in the transcript levels of proinflammatory cytokines [Interleukins (ILs) 4 and 6], respectively by ~ 6- and 4-fold in the lungs of GE mice compared to CON. Interestingly, IL-10 expression was also increased by ~ 10-fold in the lungs of GE mice suggesting an attempt to counterbalance the established inflammation. Moreover, the pulmonary expression of IL-12 and TNF-α was downregulated by 2- and 4-fold, respectively, suggesting the skewing toward Th2 phenotype. Additionally, GE mice showed a significant upregulation in Bax/Bcl-2 ratio, caspases 3, 8, and 9 with no change in JNK expression in the lungs, suggesting the activation of both intrinsic and extrinsic apoptotic pathways. Static gasoline exposure over seven consecutive weeks had a minor hepatic portal inflammation attested by H&E staining along with an increase in the hepatic expression of the mitochondrial complexes in GE mice. Therefore, tissue damage biomarkers highlight the health risks associated with vapor exposure and may present potential therapeutic targets for recovery from gasoline intoxication.Published2023-01-09T13:44:35Z2023-01-09T13:44:35Z20222023-01-09Articleinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article0260-437Xhttp://hdl.handle.net/10725/14354https://doi.org/10.1002/jat.4286Nour‐Eldine, W., Sayyed, K., Harhous, Z., Dagher‐Hamalian, C., Mehanna, S., Achkouti, D., ... & Abi‐Gerges, A. (2022). Gasoline fume inhalation induces apoptosis, inflammation, and favors Th2 polarization in C57BL/6 mice. Journal of Applied Toxicology, 42(7), 1178-1191.http://libraries.lau.edu.lb/research/laur/terms-of-use/articles.phphttps://analyticalsciencejournals.onlinelibrary.wiley.com/doi/full/10.1002/jat.4286enJournal of Applied Toxicologyinfo:eu-repo/semantics/openAccessoai:laur.lau.edu.lb:10725/143542023-01-09T13:53:40Z |
| spellingShingle | Gasoline fume inhalation induces apoptosis, inflammation, and favors Th2 polarization in C57BL/6 mice Nour-Eldine, Wared |
| status_str | publishedVersion |
| title | Gasoline fume inhalation induces apoptosis, inflammation, and favors Th2 polarization in C57BL/6 mice |
| title_full | Gasoline fume inhalation induces apoptosis, inflammation, and favors Th2 polarization in C57BL/6 mice |
| title_fullStr | Gasoline fume inhalation induces apoptosis, inflammation, and favors Th2 polarization in C57BL/6 mice |
| title_full_unstemmed | Gasoline fume inhalation induces apoptosis, inflammation, and favors Th2 polarization in C57BL/6 mice |
| title_short | Gasoline fume inhalation induces apoptosis, inflammation, and favors Th2 polarization in C57BL/6 mice |
| title_sort | Gasoline fume inhalation induces apoptosis, inflammation, and favors Th2 polarization in C57BL/6 mice |
| url | http://hdl.handle.net/10725/14354 https://doi.org/10.1002/jat.4286 http://libraries.lau.edu.lb/research/laur/terms-of-use/articles.php https://analyticalsciencejournals.onlinelibrary.wiley.com/doi/full/10.1002/jat.4286 |