Rapid quantification of ruthenium(II) polypyridyl anti-cancer drugs using a selective ligand dissociation LC-MS/MS method
Research on Ru anti-cancer drugs is on the rise with many complexes in clinical trials. Inductively coupled plasma-mass spectrometry (ICP-MS) has been the standard technique for bioanalytical studies on Ru and Pt complexes in biological media. Tedious ICP-MS methods rely on detecting and quantifying...
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| المؤلف الرئيسي: | |
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| مؤلفون آخرون: | , , |
| التنسيق: | article |
| منشور في: |
2020
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| الوصول للمادة أونلاين: | http://hdl.handle.net/10725/14358 https://doi.org/10.1039/D0AY01250E http://libraries.lau.edu.lb/research/laur/terms-of-use/articles.php https://pubs.rsc.org/en/content/articlehtml/2020/ay/d0ay01250e |
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| _version_ | 1864513469271769088 |
|---|---|
| author | Mehanna, Stephanie |
| author2 | Bodman-Smith, Kikki Daher, Costantine F. Khnayzer, Rony S. |
| author2_role | author author author |
| author_facet | Mehanna, Stephanie Bodman-Smith, Kikki Daher, Costantine F. Khnayzer, Rony S. |
| author_role | author |
| dc.creator.none.fl_str_mv | Mehanna, Stephanie Bodman-Smith, Kikki Daher, Costantine F. Khnayzer, Rony S. |
| dc.date.none.fl_str_mv | 2020 2023-01-11T14:21:41Z 2023-01-11T14:21:41Z 2023-01-11 |
| dc.identifier.none.fl_str_mv | 1759-9660 http://hdl.handle.net/10725/14358 https://doi.org/10.1039/D0AY01250E Mehanna, S., Bodman-Smith, K., Daher, C. F., & Khnayzer, R. S. (2020). Rapid quantification of ruthenium (ii) polypyridyl anti-cancer drugs using a selective ligand dissociation LC-MS/MS method. Analytical Methods, 12(37), 4517-4525. http://libraries.lau.edu.lb/research/laur/terms-of-use/articles.php https://pubs.rsc.org/en/content/articlehtml/2020/ay/d0ay01250e |
| dc.language.none.fl_str_mv | en |
| dc.relation.none.fl_str_mv | Analytical Methods |
| dc.rights.*.fl_str_mv | info:eu-repo/semantics/openAccess |
| dc.title.none.fl_str_mv | Rapid quantification of ruthenium(II) polypyridyl anti-cancer drugs using a selective ligand dissociation LC-MS/MS method |
| dc.type.none.fl_str_mv | Article info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article |
| description | Research on Ru anti-cancer drugs is on the rise with many complexes in clinical trials. Inductively coupled plasma-mass spectrometry (ICP-MS) has been the standard technique for bioanalytical studies on Ru and Pt complexes in biological media. Tedious ICP-MS methods rely on detecting and quantifying the element while lacking important structural information of the original complexes. Despite being equally sensitive, more accessible, and highly selective to the target species, liquid chromatography-tandem mass spectrometry (LC-MS/MS) has not been validated for the analysis of Ru drugs. Using USFDA guidelines, we report here the optimization and validation of a facile LC-MS/MS method for the detection and quantification of three Ru(II) polypyridyl complexes in cells, plasma, and urine matrices. Importantly, a fast (10 min), single-step procedure was efficient for both extraction and sample purification, and analytes were rapidly eluted over a 3 min simple isocratic run. Specific parent ions were differentially fragmented by tandem MS, thus forming a unique and rational ligand dissociation chemistry that exhibits high selectivity to the target species with no measurable interferences or matrix effects. The developed LC-MS/MS method was advantageous vis-à-vis the prototypical ICP-MS based techniques both in vitro and in vivo, paving the way for its utilization in elaborate cellular uptake, pharmacokinetics, and pharmacodynamics studies. |
| eu_rights_str_mv | openAccess |
| format | article |
| id | LAURepo_3cc6126223fdc96dea9d6d3929e3c40d |
| identifier_str_mv | 1759-9660 Mehanna, S., Bodman-Smith, K., Daher, C. F., & Khnayzer, R. S. (2020). Rapid quantification of ruthenium (ii) polypyridyl anti-cancer drugs using a selective ligand dissociation LC-MS/MS method. Analytical Methods, 12(37), 4517-4525. |
| language_invalid_str_mv | en |
| network_acronym_str | LAURepo |
| network_name_str | Lebanese American University repository |
| oai_identifier_str | oai:laur.lau.edu.lb:10725/14358 |
| publishDate | 2020 |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| spelling | Rapid quantification of ruthenium(II) polypyridyl anti-cancer drugs using a selective ligand dissociation LC-MS/MS methodMehanna, StephanieBodman-Smith, KikkiDaher, Costantine F.Khnayzer, Rony S.Research on Ru anti-cancer drugs is on the rise with many complexes in clinical trials. Inductively coupled plasma-mass spectrometry (ICP-MS) has been the standard technique for bioanalytical studies on Ru and Pt complexes in biological media. Tedious ICP-MS methods rely on detecting and quantifying the element while lacking important structural information of the original complexes. Despite being equally sensitive, more accessible, and highly selective to the target species, liquid chromatography-tandem mass spectrometry (LC-MS/MS) has not been validated for the analysis of Ru drugs. Using USFDA guidelines, we report here the optimization and validation of a facile LC-MS/MS method for the detection and quantification of three Ru(II) polypyridyl complexes in cells, plasma, and urine matrices. Importantly, a fast (10 min), single-step procedure was efficient for both extraction and sample purification, and analytes were rapidly eluted over a 3 min simple isocratic run. Specific parent ions were differentially fragmented by tandem MS, thus forming a unique and rational ligand dissociation chemistry that exhibits high selectivity to the target species with no measurable interferences or matrix effects. The developed LC-MS/MS method was advantageous vis-à-vis the prototypical ICP-MS based techniques both in vitro and in vivo, paving the way for its utilization in elaborate cellular uptake, pharmacokinetics, and pharmacodynamics studies.Published2023-01-11T14:21:41Z2023-01-11T14:21:41Z20202023-01-11Articleinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1759-9660http://hdl.handle.net/10725/14358https://doi.org/10.1039/D0AY01250EMehanna, S., Bodman-Smith, K., Daher, C. F., & Khnayzer, R. S. (2020). Rapid quantification of ruthenium (ii) polypyridyl anti-cancer drugs using a selective ligand dissociation LC-MS/MS method. Analytical Methods, 12(37), 4517-4525.http://libraries.lau.edu.lb/research/laur/terms-of-use/articles.phphttps://pubs.rsc.org/en/content/articlehtml/2020/ay/d0ay01250eenAnalytical Methodsinfo:eu-repo/semantics/openAccessoai:laur.lau.edu.lb:10725/143582023-01-12T07:50:46Z |
| spellingShingle | Rapid quantification of ruthenium(II) polypyridyl anti-cancer drugs using a selective ligand dissociation LC-MS/MS method Mehanna, Stephanie |
| status_str | publishedVersion |
| title | Rapid quantification of ruthenium(II) polypyridyl anti-cancer drugs using a selective ligand dissociation LC-MS/MS method |
| title_full | Rapid quantification of ruthenium(II) polypyridyl anti-cancer drugs using a selective ligand dissociation LC-MS/MS method |
| title_fullStr | Rapid quantification of ruthenium(II) polypyridyl anti-cancer drugs using a selective ligand dissociation LC-MS/MS method |
| title_full_unstemmed | Rapid quantification of ruthenium(II) polypyridyl anti-cancer drugs using a selective ligand dissociation LC-MS/MS method |
| title_short | Rapid quantification of ruthenium(II) polypyridyl anti-cancer drugs using a selective ligand dissociation LC-MS/MS method |
| title_sort | Rapid quantification of ruthenium(II) polypyridyl anti-cancer drugs using a selective ligand dissociation LC-MS/MS method |
| url | http://hdl.handle.net/10725/14358 https://doi.org/10.1039/D0AY01250E http://libraries.lau.edu.lb/research/laur/terms-of-use/articles.php https://pubs.rsc.org/en/content/articlehtml/2020/ay/d0ay01250e |