Methylation profiles of DXPas34 during the onset of X-inactivation
X chromosome inactivation is controlled by the cis-acting X-inactivation centre (Xic). In addition to initiating inactivation, Xic, which includes the Xist gene, is involved in both a counting process that senses the number of X chromosomes and the choice of X chromosome to inactivate. Controlling e...
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| المؤلف الرئيسي: | |
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| مؤلفون آخرون: | , , , |
| التنسيق: | article |
| منشور في: |
2001
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| الوصول للمادة أونلاين: | http://hdl.handle.net/10725/6164 https://doi.org/10.1093/hmg/10.1.31 http://libraries.lau.edu.lb/research/laur/terms-of-use/articles.php https://academic.oup.com/hmg/article/10/1/31/708441/Methylation-profiles-of-DXPas34-during-the-onset |
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| _version_ | 1864513478618775552 |
|---|---|
| author | El Maarri, Osman |
| author2 | Prissette, Marine Arnaud, Danielle Walter, Jorn Avner, Philip |
| author2_role | author author author author |
| author_facet | El Maarri, Osman Prissette, Marine Arnaud, Danielle Walter, Jorn Avner, Philip |
| author_role | author |
| dc.creator.none.fl_str_mv | El Maarri, Osman Prissette, Marine Arnaud, Danielle Walter, Jorn Avner, Philip |
| dc.date.none.fl_str_mv | 2001 2017-09-12T10:12:33Z 2017-09-12T10:12:33Z 2017-09-12 |
| dc.identifier.none.fl_str_mv | 1460-2083 http://hdl.handle.net/10725/6164 https://doi.org/10.1093/hmg/10.1.31 Prissette, M., El-Maarri, O., Arnaud, D., Walter, J., & Avner, P. (2001). Methylation profiles of DXPas34 during the onset of X-inactivation. Human molecular genetics, 10(1), 31-38. http://libraries.lau.edu.lb/research/laur/terms-of-use/articles.php https://academic.oup.com/hmg/article/10/1/31/708441/Methylation-profiles-of-DXPas34-during-the-onset |
| dc.language.none.fl_str_mv | en |
| dc.relation.none.fl_str_mv | Human Molecular Genetics |
| dc.rights.*.fl_str_mv | info:eu-repo/semantics/openAccess |
| dc.title.none.fl_str_mv | Methylation profiles of DXPas34 during the onset of X-inactivation |
| dc.type.none.fl_str_mv | Article info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article |
| description | X chromosome inactivation is controlled by the cis-acting X-inactivation centre (Xic). In addition to initiating inactivation, Xic, which includes the Xist gene, is involved in both a counting process that senses the number of X chromosomes and the choice of X chromosome to inactivate. Controlling elements lying 3′ to Xist include the DXPas34 locus. Deletion of DXPas34 in undifferentiated embryonic stem (ES) cells eliminates expression of both Xist and the antisense transcript Tsix, thought to initiatefrom a CpG island lying close to, but telomeric to, the DXPas34 locus itself. Deletion of DXPas34 leads to non-random inactivation on ES cell differentiation and disrupts imprinted X-inactivation in vivo. In order to investigate the role of methylation at DXPas34 in the initial steps of X-inactivation, we studied its methylation status during pre- and post-implantation embryonic development and ES cell differentiation, using the bisulphite sequencing technique. Analysis of the methylation status of both the DXPas34 locus and the associated downstream CpG island shows that extensive hypermethylation of the DXPas34 locus is a relatively late event in differentiation and embryogenesis. We conclude that methylation of DXPas34 cannot be the X chromosome imprint, nor can it be involved in the parent-of-origin effects associated with deletion of the DXPas34 locus and the neighbouring CpG island. |
| eu_rights_str_mv | openAccess |
| format | article |
| id | LAURepo_409e60ae7e284fd3ffdd8160414485ce |
| identifier_str_mv | 1460-2083 Prissette, M., El-Maarri, O., Arnaud, D., Walter, J., & Avner, P. (2001). Methylation profiles of DXPas34 during the onset of X-inactivation. Human molecular genetics, 10(1), 31-38. |
| language_invalid_str_mv | en |
| network_acronym_str | LAURepo |
| network_name_str | Lebanese American University repository |
| oai_identifier_str | oai:laur.lau.edu.lb:10725/6164 |
| publishDate | 2001 |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| spelling | Methylation profiles of DXPas34 during the onset of X-inactivationEl Maarri, OsmanPrissette, MarineArnaud, DanielleWalter, JornAvner, PhilipX chromosome inactivation is controlled by the cis-acting X-inactivation centre (Xic). In addition to initiating inactivation, Xic, which includes the Xist gene, is involved in both a counting process that senses the number of X chromosomes and the choice of X chromosome to inactivate. Controlling elements lying 3′ to Xist include the DXPas34 locus. Deletion of DXPas34 in undifferentiated embryonic stem (ES) cells eliminates expression of both Xist and the antisense transcript Tsix, thought to initiatefrom a CpG island lying close to, but telomeric to, the DXPas34 locus itself. Deletion of DXPas34 leads to non-random inactivation on ES cell differentiation and disrupts imprinted X-inactivation in vivo. In order to investigate the role of methylation at DXPas34 in the initial steps of X-inactivation, we studied its methylation status during pre- and post-implantation embryonic development and ES cell differentiation, using the bisulphite sequencing technique. Analysis of the methylation status of both the DXPas34 locus and the associated downstream CpG island shows that extensive hypermethylation of the DXPas34 locus is a relatively late event in differentiation and embryogenesis. We conclude that methylation of DXPas34 cannot be the X chromosome imprint, nor can it be involved in the parent-of-origin effects associated with deletion of the DXPas34 locus and the neighbouring CpG island.PublishedN/A2017-09-12T10:12:33Z2017-09-12T10:12:33Z20012017-09-12Articleinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1460-2083http://hdl.handle.net/10725/6164https://doi.org/10.1093/hmg/10.1.31Prissette, M., El-Maarri, O., Arnaud, D., Walter, J., & Avner, P. (2001). Methylation profiles of DXPas34 during the onset of X-inactivation. Human molecular genetics, 10(1), 31-38.http://libraries.lau.edu.lb/research/laur/terms-of-use/articles.phphttps://academic.oup.com/hmg/article/10/1/31/708441/Methylation-profiles-of-DXPas34-during-the-onsetenHuman Molecular Geneticsinfo:eu-repo/semantics/openAccessoai:laur.lau.edu.lb:10725/61642021-03-19T10:00:49Z |
| spellingShingle | Methylation profiles of DXPas34 during the onset of X-inactivation El Maarri, Osman |
| status_str | publishedVersion |
| title | Methylation profiles of DXPas34 during the onset of X-inactivation |
| title_full | Methylation profiles of DXPas34 during the onset of X-inactivation |
| title_fullStr | Methylation profiles of DXPas34 during the onset of X-inactivation |
| title_full_unstemmed | Methylation profiles of DXPas34 during the onset of X-inactivation |
| title_short | Methylation profiles of DXPas34 during the onset of X-inactivation |
| title_sort | Methylation profiles of DXPas34 during the onset of X-inactivation |
| url | http://hdl.handle.net/10725/6164 https://doi.org/10.1093/hmg/10.1.31 http://libraries.lau.edu.lb/research/laur/terms-of-use/articles.php https://academic.oup.com/hmg/article/10/1/31/708441/Methylation-profiles-of-DXPas34-during-the-onset |