956. Imaging of radiation-inducible promotersu using a naturally secreted luciferase from the Marine copepod gaussia princeps
Ionizing Radiation (IR)-inducible promoters used as genetic switches for cancer therapy are promising tool for controlling therapeutic gene expression. Here we describe the use of a naturally secreted luciferase from the marine copepod Gaussia princeps (Gluc) for monitoring the activity and inductio...
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| المؤلف الرئيسي: | |
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| مؤلفون آخرون: | , , |
| التنسيق: | article |
| منشور في: |
2006
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| الوصول للمادة أونلاين: | http://hdl.handle.net/10725/11121 https://doi.org/10.1016/j.ymthe.2006.08.1048 http://libraries.lau.edu.lb/research/laur/terms-of-use/articles.php https://www.cell.com/molecular-therapy-family/molecular-therapy/fulltext/S1525-0016(06)01310-4 |
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| الملخص: | Ionizing Radiation (IR)-inducible promoters used as genetic switches for cancer therapy are promising tool for controlling therapeutic gene expression. Here we describe the use of a naturally secreted luciferase from the marine copepod Gaussia princeps (Gluc) for monitoring the activity and induction levels of different IR- sensitive promoters. The following promoters were tested: wild-type p53-activated fragment 1 (WAF1, 2.4 kb); early growth response factor (Egr-1, 550 bp); four tandem repeats of the transcriptional factor nuclear factor-kB (4NF-kB, 400 bp); and a combination of both Egr-1 and 4NF-kB promoters. These promoters were cloned upstream of the humanized Gluc cDNA in a plasmid containing the eGFP gene under the control of an immediate-early herpes simplex virus type-1 promoter. Vero2-2 (African green monkey kidney cells) were transfected with each of these constructs and irradiated at different doses of g-rays. Activity of these promoters and their response to radiation was monitored overtime by taking an aliquot of the cell-free conditioned medium, adding coelenterazine, the Gluc substrate, and measuring bioluminescence. At-least two- fold induction was detected with each promoter as observed by bioluminescence imaging of Gluc. This system can be used to monitor, in real-time, the activity as well as the effectiveness of radiation-inducible promoters to drive the expression of toxic genes for cancer therapy. |
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