Protein and pH-dependent Binding of Nascent Pectin and Glucuronarabinoxylan to Xylogucan in Pea
Nascent pectin and glucuronoarabinoxylan, synthesised in vitro by membrane-bound enzymes from etiolated pea (Pisum sativum L.) epicotyls, were found to bind to pea xyloglucan in a pH-dependent manner. The binding was maximum at low pH (3–4), and decreased to almost zero at pH 6. The binding was pro...
محفوظ في:
| المؤلف الرئيسي: | |
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| مؤلفون آخرون: | , , |
| التنسيق: | article |
| منشور في: |
2000
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| الوصول للمادة أونلاين: | http://hdl.handle.net/10725/2339 http://dx.doi.org/10.1007/s004250000303 http://link.springer.com/article/10.1007/s004250000303 |
| الوسوم: |
إضافة وسم
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| _version_ | 1864513457940856832 |
|---|---|
| author | Rizk, Sandra |
| author2 | Abdel-Massih, Roula Baydoun, Elias Brett, Christopher |
| author2_role | author author author |
| author_facet | Rizk, Sandra Abdel-Massih, Roula Baydoun, Elias Brett, Christopher |
| author_role | author |
| dc.creator.none.fl_str_mv | Rizk, Sandra Abdel-Massih, Roula Baydoun, Elias Brett, Christopher |
| dc.date.none.fl_str_mv | 2000 2015-10-26T11:23:12Z 2015-10-26T11:23:12Z 2015-10-26 |
| dc.identifier.none.fl_str_mv | 0032-0935 http://hdl.handle.net/10725/2339 http://dx.doi.org/10.1007/s004250000303 Rizk, S. E., Abdel-Massih, R. M., Baydoun, E. A. H., & Brett, C. T. (2000). Protein-and pH-dependent binding of nascent pectin and glucuronoarabinoxylan to xyloglucan in pea. Planta, 211(3), 423-429. http://link.springer.com/article/10.1007/s004250000303 |
| dc.language.none.fl_str_mv | en |
| dc.relation.none.fl_str_mv | Planta |
| dc.rights.*.fl_str_mv | info:eu-repo/semantics/openAccess |
| dc.title.none.fl_str_mv | Protein and pH-dependent Binding of Nascent Pectin and Glucuronarabinoxylan to Xylogucan in Pea |
| dc.type.none.fl_str_mv | Article info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article |
| description | Nascent pectin and glucuronoarabinoxylan, synthesised in vitro by membrane-bound enzymes from etiolated pea (Pisum sativum L.) epicotyls, were found to bind to pea xyloglucan in a pH-dependent manner. The binding was maximum at low pH (3–4), and decreased to almost zero at pH 6. The binding was probably non-covalent and reached saturation within 5 min. Removal of the fucose residues of xyloglucan decreased the degree of binding. Removal by protease of the proteins attached to nascent pectin and glucuronoarabinoxylan greatly reduced the maximum binding and abolished the pH-dependence. The observed binding may be of considerable significance in the process of cell-wall assembly and in the control of cell extension. |
| eu_rights_str_mv | openAccess |
| format | article |
| id | LAURepo_433ba88b387fbb1bd2472bceb9e58232 |
| identifier_str_mv | 0032-0935 Rizk, S. E., Abdel-Massih, R. M., Baydoun, E. A. H., & Brett, C. T. (2000). Protein-and pH-dependent binding of nascent pectin and glucuronoarabinoxylan to xyloglucan in pea. Planta, 211(3), 423-429. |
| language_invalid_str_mv | en |
| network_acronym_str | LAURepo |
| network_name_str | Lebanese American University repository |
| oai_identifier_str | oai:laur.lau.edu.lb:10725/2339 |
| publishDate | 2000 |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| spelling | Protein and pH-dependent Binding of Nascent Pectin and Glucuronarabinoxylan to Xylogucan in PeaRizk, SandraAbdel-Massih, RoulaBaydoun, EliasBrett, Christopher Nascent pectin and glucuronoarabinoxylan, synthesised in vitro by membrane-bound enzymes from etiolated pea (Pisum sativum L.) epicotyls, were found to bind to pea xyloglucan in a pH-dependent manner. The binding was maximum at low pH (3–4), and decreased to almost zero at pH 6. The binding was probably non-covalent and reached saturation within 5 min. Removal of the fucose residues of xyloglucan decreased the degree of binding. Removal by protease of the proteins attached to nascent pectin and glucuronoarabinoxylan greatly reduced the maximum binding and abolished the pH-dependence. The observed binding may be of considerable significance in the process of cell-wall assembly and in the control of cell extension.PublishedN/A2015-10-26T11:23:12Z2015-10-26T11:23:12Z20002015-10-26Articleinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article0032-0935http://hdl.handle.net/10725/2339http://dx.doi.org/10.1007/s004250000303Rizk, S. E., Abdel-Massih, R. M., Baydoun, E. A. H., & Brett, C. T. (2000). Protein-and pH-dependent binding of nascent pectin and glucuronoarabinoxylan to xyloglucan in pea. Planta, 211(3), 423-429.http://link.springer.com/article/10.1007/s004250000303enPlantainfo:eu-repo/semantics/openAccessoai:laur.lau.edu.lb:10725/23392016-07-22T08:12:14Z |
| spellingShingle | Protein and pH-dependent Binding of Nascent Pectin and Glucuronarabinoxylan to Xylogucan in Pea Rizk, Sandra |
| status_str | publishedVersion |
| title | Protein and pH-dependent Binding of Nascent Pectin and Glucuronarabinoxylan to Xylogucan in Pea |
| title_full | Protein and pH-dependent Binding of Nascent Pectin and Glucuronarabinoxylan to Xylogucan in Pea |
| title_fullStr | Protein and pH-dependent Binding of Nascent Pectin and Glucuronarabinoxylan to Xylogucan in Pea |
| title_full_unstemmed | Protein and pH-dependent Binding of Nascent Pectin and Glucuronarabinoxylan to Xylogucan in Pea |
| title_short | Protein and pH-dependent Binding of Nascent Pectin and Glucuronarabinoxylan to Xylogucan in Pea |
| title_sort | Protein and pH-dependent Binding of Nascent Pectin and Glucuronarabinoxylan to Xylogucan in Pea |
| url | http://hdl.handle.net/10725/2339 http://dx.doi.org/10.1007/s004250000303 http://link.springer.com/article/10.1007/s004250000303 |