Human recombinant arginase I(Co)-PEG5000 [HuArgI(Co)-PEG5000]-induced arginine depletion is selectively cytotoxic to human acute myeloid leukemia cells
In this study, we target arginine auxotrophy of AML cell lines using human arginase I cobalt-PEG5000. HuArgI(Co)-PEG5000 was cytotoxic to all AML cell lines tested. Mononuclear cells and CD34+ blasts were not sensitive demonstrating the selectivity of HuArgI(Co)-PEG5000-induced arginine deprivation....
محفوظ في:
| المؤلف الرئيسي: | |
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| مؤلفون آخرون: | , , , , , |
| التنسيق: | article |
| منشور في: |
2013
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| الوصول للمادة أونلاين: | http://hdl.handle.net/10725/2741 http://dx.doi.org/10.1016/j.leukres.2013.08.007 http://www.sciencedirect.com/science/article/pii/S0145212613002889 |
| الوسوم: |
إضافة وسم
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| _version_ | 1864513459134136320 |
|---|---|
| author | Bekdash, Amira |
| author2 | Abi-Habib, Ralph J. Tanios, Rita Stone, Everett Georgiou, Georges Frankel, Arthur E. Kassab, Elias |
| author2_role | author author author author author author |
| author_facet | Bekdash, Amira Abi-Habib, Ralph J. Tanios, Rita Stone, Everett Georgiou, Georges Frankel, Arthur E. Kassab, Elias |
| author_role | author |
| dc.creator.none.fl_str_mv | Bekdash, Amira Abi-Habib, Ralph J. Tanios, Rita Stone, Everett Georgiou, Georges Frankel, Arthur E. Kassab, Elias |
| dc.date.none.fl_str_mv | 2013 2015-12-01T08:20:38Z 2015-12-01T08:20:38Z 2015-12-01 |
| dc.identifier.none.fl_str_mv | 0145-2126 http://hdl.handle.net/10725/2741 http://dx.doi.org/10.1016/j.leukres.2013.08.007 Tanios, R., Bekdash, A., Kassab, E., Stone, E., Georgiou, G., Frankel, A. E., & Abi-Habib, R. J. (2013). Human recombinant arginase I (Co)-PEG5000 [HuArgI (Co)-PEG5000]-induced arginine depletion is selectively cytotoxic to human acute myeloid leukemia cells. Leukemia research, 37(11), 1565-1571. http://www.sciencedirect.com/science/article/pii/S0145212613002889 |
| dc.language.none.fl_str_mv | en |
| dc.relation.none.fl_str_mv | Leukemia Research |
| dc.rights.*.fl_str_mv | info:eu-repo/semantics/openAccess |
| dc.title.none.fl_str_mv | Human recombinant arginase I(Co)-PEG5000 [HuArgI(Co)-PEG5000]-induced arginine depletion is selectively cytotoxic to human acute myeloid leukemia cells |
| dc.type.none.fl_str_mv | Article info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article |
| description | In this study, we target arginine auxotrophy of AML cell lines using human arginase I cobalt-PEG5000. HuArgI(Co)-PEG5000 was cytotoxic to all AML cell lines tested. Mononuclear cells and CD34+ blasts were not sensitive demonstrating the selectivity of HuArgI(Co)-PEG5000-induced arginine deprivation. Addition of l-citrulline led to the rescue of five cell lines. The four cell lines that were not rescued by l-citrulline did not express argininosuccinate synthetase-1, indicating complete arginine auxotrophy. Inhibition of autophagy increased cell sensitivity to HuArgI(Co)-PEG5000 demonstrating the protective role of autophagy following arginine deprivation. We have shown that AML can be selectively targeted using HuArgI(Co)-PEG5000-induced arginine depletion. |
| eu_rights_str_mv | openAccess |
| format | article |
| id | LAURepo_4ba76f3cb2f24a62dba967adc15d69a5 |
| identifier_str_mv | 0145-2126 Tanios, R., Bekdash, A., Kassab, E., Stone, E., Georgiou, G., Frankel, A. E., & Abi-Habib, R. J. (2013). Human recombinant arginase I (Co)-PEG5000 [HuArgI (Co)-PEG5000]-induced arginine depletion is selectively cytotoxic to human acute myeloid leukemia cells. Leukemia research, 37(11), 1565-1571. |
| language_invalid_str_mv | en |
| network_acronym_str | LAURepo |
| network_name_str | Lebanese American University repository |
| oai_identifier_str | oai:laur.lau.edu.lb:10725/2741 |
| publishDate | 2013 |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| spelling | Human recombinant arginase I(Co)-PEG5000 [HuArgI(Co)-PEG5000]-induced arginine depletion is selectively cytotoxic to human acute myeloid leukemia cellsBekdash, AmiraAbi-Habib, Ralph J.Tanios, RitaStone, EverettGeorgiou, GeorgesFrankel, Arthur E.Kassab, EliasIn this study, we target arginine auxotrophy of AML cell lines using human arginase I cobalt-PEG5000. HuArgI(Co)-PEG5000 was cytotoxic to all AML cell lines tested. Mononuclear cells and CD34+ blasts were not sensitive demonstrating the selectivity of HuArgI(Co)-PEG5000-induced arginine deprivation. Addition of l-citrulline led to the rescue of five cell lines. The four cell lines that were not rescued by l-citrulline did not express argininosuccinate synthetase-1, indicating complete arginine auxotrophy. Inhibition of autophagy increased cell sensitivity to HuArgI(Co)-PEG5000 demonstrating the protective role of autophagy following arginine deprivation. We have shown that AML can be selectively targeted using HuArgI(Co)-PEG5000-induced arginine depletion.PublishedN/A2015-12-01T08:20:38Z2015-12-01T08:20:38Z20132015-12-01Articleinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article0145-2126http://hdl.handle.net/10725/2741http://dx.doi.org/10.1016/j.leukres.2013.08.007Tanios, R., Bekdash, A., Kassab, E., Stone, E., Georgiou, G., Frankel, A. E., & Abi-Habib, R. J. (2013). Human recombinant arginase I (Co)-PEG5000 [HuArgI (Co)-PEG5000]-induced arginine depletion is selectively cytotoxic to human acute myeloid leukemia cells. Leukemia research, 37(11), 1565-1571.http://www.sciencedirect.com/science/article/pii/S0145212613002889enLeukemia Researchinfo:eu-repo/semantics/openAccessoai:laur.lau.edu.lb:10725/27412016-08-30T06:54:00Z |
| spellingShingle | Human recombinant arginase I(Co)-PEG5000 [HuArgI(Co)-PEG5000]-induced arginine depletion is selectively cytotoxic to human acute myeloid leukemia cells Bekdash, Amira |
| status_str | publishedVersion |
| title | Human recombinant arginase I(Co)-PEG5000 [HuArgI(Co)-PEG5000]-induced arginine depletion is selectively cytotoxic to human acute myeloid leukemia cells |
| title_full | Human recombinant arginase I(Co)-PEG5000 [HuArgI(Co)-PEG5000]-induced arginine depletion is selectively cytotoxic to human acute myeloid leukemia cells |
| title_fullStr | Human recombinant arginase I(Co)-PEG5000 [HuArgI(Co)-PEG5000]-induced arginine depletion is selectively cytotoxic to human acute myeloid leukemia cells |
| title_full_unstemmed | Human recombinant arginase I(Co)-PEG5000 [HuArgI(Co)-PEG5000]-induced arginine depletion is selectively cytotoxic to human acute myeloid leukemia cells |
| title_short | Human recombinant arginase I(Co)-PEG5000 [HuArgI(Co)-PEG5000]-induced arginine depletion is selectively cytotoxic to human acute myeloid leukemia cells |
| title_sort | Human recombinant arginase I(Co)-PEG5000 [HuArgI(Co)-PEG5000]-induced arginine depletion is selectively cytotoxic to human acute myeloid leukemia cells |
| url | http://hdl.handle.net/10725/2741 http://dx.doi.org/10.1016/j.leukres.2013.08.007 http://www.sciencedirect.com/science/article/pii/S0145212613002889 |