The Effect of Recombinant Anthrax Lethal Toxin on Breast Cancer Cell Motility, Adhesion, and Invasion
Breast cancer-related deaths are mostly due to breast cancer invasion and migration to distant secondary regions. Targeting breast cancer cell metastasis is an important therapeutic approach. The mitogen-activated protein kinase (MAPK) pathway is a key cell signaling pathway that plays a major role...
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| Format: | masterThesis |
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2020
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| Online Access: | http://hdl.handle.net/10725/13838 https://doi.org/10.26756/th.2022.364 http://libraries.lau.edu.lb/research/laur/terms-of-use/thesis.php |
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| _version_ | 1864513468306030592 |
|---|---|
| author | El Chami, Dana |
| author_facet | El Chami, Dana |
| author_role | author |
| dc.creator.none.fl_str_mv | El Chami, Dana |
| dc.date.none.fl_str_mv | 2020 2020-05-18 2022-07-20T06:23:25Z 2022-07-20T06:23:25Z |
| dc.identifier.none.fl_str_mv | http://hdl.handle.net/10725/13838 https://doi.org/10.26756/th.2022.364 http://libraries.lau.edu.lb/research/laur/terms-of-use/thesis.php |
| dc.language.none.fl_str_mv | en |
| dc.publisher.none.fl_str_mv | Lebanese American University |
| dc.rights.*.fl_str_mv | info:eu-repo/semantics/openAccess |
| dc.subject.none.fl_str_mv | Breast -- Cancer -- Molecular aspects Cancer cells -- Motility Mitogen-activated protein kinases Lebanese American University -- Dissertations Dissertations, Academic |
| dc.title.none.fl_str_mv | The Effect of Recombinant Anthrax Lethal Toxin on Breast Cancer Cell Motility, Adhesion, and Invasion |
| dc.type.none.fl_str_mv | Thesis info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/masterThesis |
| description | Breast cancer-related deaths are mostly due to breast cancer invasion and migration to distant secondary regions. Targeting breast cancer cell metastasis is an important therapeutic approach. The mitogen-activated protein kinase (MAPK) pathway is a key cell signaling pathway that plays a major role in cell invasion and migration. Many studies have targeted the MAPK pathway as a way to target cell survival and motility. In this study, we use Lethal Toxin (LT) a potent MAPK inhibitor that selectively inactivates all the kinases in the MAPK pathway. LT proved to affect breast cancer cell migration, adhesion, and invasion. Cells treated with LT showed a significant decrease in motility as seen in 2D time-lapse and wound healing assays. Additionally, cells treated with LT showed an increase in adhesion, decrease in invasion across a collagen matrix, and in increase in Rho A activation. We speculate that LT inhibited cell migration by deregulating the activity of Rho GTPases, proteins known to play a role in cell migration. In this study, we describe the effect of LT as a potential breast cancer cell invasion and motility inhibitor. |
| eu_rights_str_mv | openAccess |
| format | masterThesis |
| id | LAURepo_4edaf405cc1a0af20bb254f726a6f144 |
| language_invalid_str_mv | en |
| network_acronym_str | LAURepo |
| network_name_str | Lebanese American University repository |
| oai_identifier_str | oai:laur.lau.edu.lb:10725/13838 |
| publishDate | 2020 |
| publisher.none.fl_str_mv | Lebanese American University |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
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| spelling | The Effect of Recombinant Anthrax Lethal Toxin on Breast Cancer Cell Motility, Adhesion, and InvasionEl Chami, DanaBreast -- Cancer -- Molecular aspectsCancer cells -- MotilityMitogen-activated protein kinasesLebanese American University -- DissertationsDissertations, AcademicBreast cancer-related deaths are mostly due to breast cancer invasion and migration to distant secondary regions. Targeting breast cancer cell metastasis is an important therapeutic approach. The mitogen-activated protein kinase (MAPK) pathway is a key cell signaling pathway that plays a major role in cell invasion and migration. Many studies have targeted the MAPK pathway as a way to target cell survival and motility. In this study, we use Lethal Toxin (LT) a potent MAPK inhibitor that selectively inactivates all the kinases in the MAPK pathway. LT proved to affect breast cancer cell migration, adhesion, and invasion. Cells treated with LT showed a significant decrease in motility as seen in 2D time-lapse and wound healing assays. Additionally, cells treated with LT showed an increase in adhesion, decrease in invasion across a collagen matrix, and in increase in Rho A activation. We speculate that LT inhibited cell migration by deregulating the activity of Rho GTPases, proteins known to play a role in cell migration. In this study, we describe the effect of LT as a potential breast cancer cell invasion and motility inhibitor.1 online resource (xii, 42 leaves): col. ill.Rare Book or ManuscriptLebanese American University2022-07-20T06:23:25Z2022-07-20T06:23:25Z20202020-05-18Thesisinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesishttp://hdl.handle.net/10725/13838https://doi.org/10.26756/th.2022.364http://libraries.lau.edu.lb/research/laur/terms-of-use/thesis.phpeninfo:eu-repo/semantics/openAccessoai:laur.lau.edu.lb:10725/138382022-07-20T06:24:28Z |
| spellingShingle | The Effect of Recombinant Anthrax Lethal Toxin on Breast Cancer Cell Motility, Adhesion, and Invasion El Chami, Dana Breast -- Cancer -- Molecular aspects Cancer cells -- Motility Mitogen-activated protein kinases Lebanese American University -- Dissertations Dissertations, Academic |
| status_str | publishedVersion |
| title | The Effect of Recombinant Anthrax Lethal Toxin on Breast Cancer Cell Motility, Adhesion, and Invasion |
| title_full | The Effect of Recombinant Anthrax Lethal Toxin on Breast Cancer Cell Motility, Adhesion, and Invasion |
| title_fullStr | The Effect of Recombinant Anthrax Lethal Toxin on Breast Cancer Cell Motility, Adhesion, and Invasion |
| title_full_unstemmed | The Effect of Recombinant Anthrax Lethal Toxin on Breast Cancer Cell Motility, Adhesion, and Invasion |
| title_short | The Effect of Recombinant Anthrax Lethal Toxin on Breast Cancer Cell Motility, Adhesion, and Invasion |
| title_sort | The Effect of Recombinant Anthrax Lethal Toxin on Breast Cancer Cell Motility, Adhesion, and Invasion |
| topic | Breast -- Cancer -- Molecular aspects Cancer cells -- Motility Mitogen-activated protein kinases Lebanese American University -- Dissertations Dissertations, Academic |
| url | http://hdl.handle.net/10725/13838 https://doi.org/10.26756/th.2022.364 http://libraries.lau.edu.lb/research/laur/terms-of-use/thesis.php |