Malva pseudolavatera Leaf Extract Promotes ROS Induction Leading to Apoptosis in Acute Myeloid Leukemia Cells In Vitro
Malva pseudolavatera Webb & Berthel. is a plant from the Malvaceae family that has long been included in the human diet due to its various curative effects. Many plant leaf extracts from the various species of Malva genus have been reported to possess anti-cancer properties, however, studies on...
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| المؤلف الرئيسي: | |
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| مؤلفون آخرون: | , , , , , |
| التنسيق: | article |
| منشور في: |
2020
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| الوصول للمادة أونلاين: | http://hdl.handle.net/10725/12021 https://doi.org/10.3390/cancers12020435 http://libraries.lau.edu.lb/research/laur/terms-of-use/articles.php https://www.mdpi.com/2072-6694/12/2/435/htm |
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| _version_ | 1864513489686495232 |
|---|---|
| author | El Khoury, Marianne |
| author2 | Haykal, Tony Hodroj, Mohammad H. Abou Najem, Sonia Sarkis, Rita Taleb, Robin I. Rizk, Sandra |
| author2_role | author author author author author author |
| author_facet | El Khoury, Marianne Haykal, Tony Hodroj, Mohammad H. Abou Najem, Sonia Sarkis, Rita Taleb, Robin I. Rizk, Sandra |
| author_role | author |
| dc.creator.none.fl_str_mv | El Khoury, Marianne Haykal, Tony Hodroj, Mohammad H. Abou Najem, Sonia Sarkis, Rita Taleb, Robin I. Rizk, Sandra |
| dc.date.none.fl_str_mv | 2020-07-23T09:10:51Z 2020-07-23T09:10:51Z 2020 2020-07-23 |
| dc.identifier.none.fl_str_mv | 2072-6694 http://hdl.handle.net/10725/12021 https://doi.org/10.3390/cancers12020435 El Khoury, M., Haykal, T., Hodroj, M. H., Najem, S. A., Sarkis, R., Taleb, R. I., & Rizk, S. (2020). Malva pseudolavatera Leaf Extract Promotes ROS Induction Leading to Apoptosis in Acute Myeloid Leukemia Cells In Vitro. Cancers, 12(2), 435. http://libraries.lau.edu.lb/research/laur/terms-of-use/articles.php https://www.mdpi.com/2072-6694/12/2/435/htm |
| dc.language.none.fl_str_mv | en |
| dc.relation.none.fl_str_mv | Cancers |
| dc.rights.*.fl_str_mv | info:eu-repo/semantics/openAccess |
| dc.title.none.fl_str_mv | Malva pseudolavatera Leaf Extract Promotes ROS Induction Leading to Apoptosis in Acute Myeloid Leukemia Cells In Vitro |
| dc.type.none.fl_str_mv | Article info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article |
| description | Malva pseudolavatera Webb & Berthel. is a plant from the Malvaceae family that has long been included in the human diet due to its various curative effects. Many plant leaf extracts from the various species of Malva genus have been reported to possess anti-cancer properties, however, studies on M. pseudolavatera Webb & Berthel. leaves have documented anti-inflammatory and anti-oxidant effects with no emphasis on their possible anti-cancer potential. The present study explores the anti-cancer properties of Malva pseudolavatera Webb & Berthel. leaf extract on acute myeloid leukemia (AML) cell lines in vitro and deciphers the underlying molecular mechanism. Treatment of AML cell lines with M. pseudolavatera methanolic leaf extract showed a dose- and time-dependent inhibition of proliferation and a dose-dependent increase in apoptotic hallmarks such as an increase in phosphatidylserine on the outer membrane leaflet and membrane leakage in addition to DNA fragmentation. The pro-apoptotic effect was induced by reactive oxygen species (ROS) as well as an upregulation of cleaved poly(ADP-ribose) polymerase (PARP), increase in Bax/Bcl-2 ratio, andrelease of cytochrome-c from the mitochondria. Major compounds of the extract included methyl linolenate, phytol, γ-sitosterol, and stigmasterol as revealed by gas chromatography coupled with mass spectrometry, and amino acids, amino acid derivatives, tiliroside, 13-hydroxyperoxyoctadecadienoic, and quercitrin as detected by liquid chromatography coupled to mass spectrometry. |
| eu_rights_str_mv | openAccess |
| format | article |
| id | LAURepo_62be4e0a267bfacab5fd3ce4c113ae54 |
| identifier_str_mv | 2072-6694 El Khoury, M., Haykal, T., Hodroj, M. H., Najem, S. A., Sarkis, R., Taleb, R. I., & Rizk, S. (2020). Malva pseudolavatera Leaf Extract Promotes ROS Induction Leading to Apoptosis in Acute Myeloid Leukemia Cells In Vitro. Cancers, 12(2), 435. |
| language_invalid_str_mv | en |
| network_acronym_str | LAURepo |
| network_name_str | Lebanese American University repository |
| oai_identifier_str | oai:laur.lau.edu.lb:10725/12021 |
| publishDate | 2020 |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| spelling | Malva pseudolavatera Leaf Extract Promotes ROS Induction Leading to Apoptosis in Acute Myeloid Leukemia Cells In VitroEl Khoury, MarianneHaykal, TonyHodroj, Mohammad H.Abou Najem, SoniaSarkis, RitaTaleb, Robin I.Rizk, SandraMalva pseudolavatera Webb & Berthel. is a plant from the Malvaceae family that has long been included in the human diet due to its various curative effects. Many plant leaf extracts from the various species of Malva genus have been reported to possess anti-cancer properties, however, studies on M. pseudolavatera Webb & Berthel. leaves have documented anti-inflammatory and anti-oxidant effects with no emphasis on their possible anti-cancer potential. The present study explores the anti-cancer properties of Malva pseudolavatera Webb & Berthel. leaf extract on acute myeloid leukemia (AML) cell lines in vitro and deciphers the underlying molecular mechanism. Treatment of AML cell lines with M. pseudolavatera methanolic leaf extract showed a dose- and time-dependent inhibition of proliferation and a dose-dependent increase in apoptotic hallmarks such as an increase in phosphatidylserine on the outer membrane leaflet and membrane leakage in addition to DNA fragmentation. The pro-apoptotic effect was induced by reactive oxygen species (ROS) as well as an upregulation of cleaved poly(ADP-ribose) polymerase (PARP), increase in Bax/Bcl-2 ratio, andrelease of cytochrome-c from the mitochondria. Major compounds of the extract included methyl linolenate, phytol, γ-sitosterol, and stigmasterol as revealed by gas chromatography coupled with mass spectrometry, and amino acids, amino acid derivatives, tiliroside, 13-hydroxyperoxyoctadecadienoic, and quercitrin as detected by liquid chromatography coupled to mass spectrometry.PublishedN/A2020-07-23T09:10:51Z2020-07-23T09:10:51Z20202020-07-23Articleinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article2072-6694http://hdl.handle.net/10725/12021https://doi.org/10.3390/cancers12020435El Khoury, M., Haykal, T., Hodroj, M. H., Najem, S. A., Sarkis, R., Taleb, R. I., & Rizk, S. (2020). Malva pseudolavatera Leaf Extract Promotes ROS Induction Leading to Apoptosis in Acute Myeloid Leukemia Cells In Vitro. Cancers, 12(2), 435.http://libraries.lau.edu.lb/research/laur/terms-of-use/articles.phphttps://www.mdpi.com/2072-6694/12/2/435/htmenCancersinfo:eu-repo/semantics/openAccessoai:laur.lau.edu.lb:10725/120212021-03-19T10:47:40Z |
| spellingShingle | Malva pseudolavatera Leaf Extract Promotes ROS Induction Leading to Apoptosis in Acute Myeloid Leukemia Cells In Vitro El Khoury, Marianne |
| status_str | publishedVersion |
| title | Malva pseudolavatera Leaf Extract Promotes ROS Induction Leading to Apoptosis in Acute Myeloid Leukemia Cells In Vitro |
| title_full | Malva pseudolavatera Leaf Extract Promotes ROS Induction Leading to Apoptosis in Acute Myeloid Leukemia Cells In Vitro |
| title_fullStr | Malva pseudolavatera Leaf Extract Promotes ROS Induction Leading to Apoptosis in Acute Myeloid Leukemia Cells In Vitro |
| title_full_unstemmed | Malva pseudolavatera Leaf Extract Promotes ROS Induction Leading to Apoptosis in Acute Myeloid Leukemia Cells In Vitro |
| title_short | Malva pseudolavatera Leaf Extract Promotes ROS Induction Leading to Apoptosis in Acute Myeloid Leukemia Cells In Vitro |
| title_sort | Malva pseudolavatera Leaf Extract Promotes ROS Induction Leading to Apoptosis in Acute Myeloid Leukemia Cells In Vitro |
| url | http://hdl.handle.net/10725/12021 https://doi.org/10.3390/cancers12020435 http://libraries.lau.edu.lb/research/laur/terms-of-use/articles.php https://www.mdpi.com/2072-6694/12/2/435/htm |