Microbiological Quality and Genotypic Speciation of Heterotrophic Bacteria Isolated from Potable Water Stored in Household Tanks

Upon storage, a statistically significant increase in heterotrophic plate count (HPC) was detected in samples from household tanks. The most consistent correlate of regrowth was found to be storage time, however, temperature and the microbiological quality of the influent water were also important....

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محفوظ في:
التفاصيل البيبلوغرافية
المؤلف الرئيسي: Tokajian, Sima (author)
مؤلفون آخرون: Hashwa, Fuad (author)
التنسيق: article
منشور في: 2004
الوصول للمادة أونلاين:http://hdl.handle.net/10725/3399
https://www.cawq.ca/journal/temp/journal/3.pdf#page=70
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author Tokajian, Sima
author2 Hashwa, Fuad
author2_role author
author_facet Tokajian, Sima
Hashwa, Fuad
author_role author
dc.creator.none.fl_str_mv Tokajian, Sima
Hashwa, Fuad
dc.date.none.fl_str_mv 2004
2016-03-23T11:59:57Z
2016-03-23T11:59:57Z
2016-03-23
dc.identifier.none.fl_str_mv 1201-3080
http://hdl.handle.net/10725/3399
Tokajian, S., & Hashwa, F. (2004). Microbiological quality and genotypic speciation of heterotrophic bacteria isolated from potable water stored in household tanks. Water quality research journal of Canada, 39(1), 64-73.
https://www.cawq.ca/journal/temp/journal/3.pdf#page=70
dc.language.none.fl_str_mv en
dc.relation.none.fl_str_mv Water Quality Research Journal of Canada
dc.rights.*.fl_str_mv info:eu-repo/semantics/openAccess
dc.title.none.fl_str_mv Microbiological Quality and Genotypic Speciation of Heterotrophic Bacteria Isolated from Potable Water Stored in Household Tanks
dc.type.none.fl_str_mv Article
info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/article
description Upon storage, a statistically significant increase in heterotrophic plate count (HPC) was detected in samples from household tanks. The most consistent correlate of regrowth was found to be storage time, however, temperature and the microbiological quality of the influent water were also important. Total and fecal coliforms were detected in the influent water and in the household storage tanks. Coliforms growing at 37ºC were a small but significant part (about 0.002%) of the total heterotrophic population. In the influent, 92% of the coliforms (11 colony forming units/100 mL) at 44ºC were Escherichia coli, while in the household tanks it was 86%. The majority of isolates from the influent and the household tanks recovered on R2A agar formed pigmented colonies and were Gram-negative belonging to the α-, β-, and γ-subclasses of Proteobacteria. Overall, the α-subclass was the dominant type representing 61% of the recovered HPC, and 40 to 50% of the organisms based on 16S rDNA sequence analysis were members of the family Sphingomonadaceae. The presence of E. coli and the isolation of opportunistic pathogens as a significant part of the HPC population from both influent and household tanks indicated a potential health hazard to consumers.
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identifier_str_mv 1201-3080
Tokajian, S., & Hashwa, F. (2004). Microbiological quality and genotypic speciation of heterotrophic bacteria isolated from potable water stored in household tanks. Water quality research journal of Canada, 39(1), 64-73.
language_invalid_str_mv en
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spelling Microbiological Quality and Genotypic Speciation of Heterotrophic Bacteria Isolated from Potable Water Stored in Household TanksTokajian, SimaHashwa, FuadUpon storage, a statistically significant increase in heterotrophic plate count (HPC) was detected in samples from household tanks. The most consistent correlate of regrowth was found to be storage time, however, temperature and the microbiological quality of the influent water were also important. Total and fecal coliforms were detected in the influent water and in the household storage tanks. Coliforms growing at 37ºC were a small but significant part (about 0.002%) of the total heterotrophic population. In the influent, 92% of the coliforms (11 colony forming units/100 mL) at 44ºC were Escherichia coli, while in the household tanks it was 86%. The majority of isolates from the influent and the household tanks recovered on R2A agar formed pigmented colonies and were Gram-negative belonging to the α-, β-, and γ-subclasses of Proteobacteria. Overall, the α-subclass was the dominant type representing 61% of the recovered HPC, and 40 to 50% of the organisms based on 16S rDNA sequence analysis were members of the family Sphingomonadaceae. The presence of E. coli and the isolation of opportunistic pathogens as a significant part of the HPC population from both influent and household tanks indicated a potential health hazard to consumers.PublishedN/A2016-03-23T11:59:57Z2016-03-23T11:59:57Z20042016-03-23Articleinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1201-3080http://hdl.handle.net/10725/3399Tokajian, S., & Hashwa, F. (2004). Microbiological quality and genotypic speciation of heterotrophic bacteria isolated from potable water stored in household tanks. Water quality research journal of Canada, 39(1), 64-73.https://www.cawq.ca/journal/temp/journal/3.pdf#page=70enWater Quality Research Journal of Canadainfo:eu-repo/semantics/openAccessoai:laur.lau.edu.lb:10725/33992018-05-23T05:57:15Z
spellingShingle Microbiological Quality and Genotypic Speciation of Heterotrophic Bacteria Isolated from Potable Water Stored in Household Tanks
Tokajian, Sima
status_str publishedVersion
title Microbiological Quality and Genotypic Speciation of Heterotrophic Bacteria Isolated from Potable Water Stored in Household Tanks
title_full Microbiological Quality and Genotypic Speciation of Heterotrophic Bacteria Isolated from Potable Water Stored in Household Tanks
title_fullStr Microbiological Quality and Genotypic Speciation of Heterotrophic Bacteria Isolated from Potable Water Stored in Household Tanks
title_full_unstemmed Microbiological Quality and Genotypic Speciation of Heterotrophic Bacteria Isolated from Potable Water Stored in Household Tanks
title_short Microbiological Quality and Genotypic Speciation of Heterotrophic Bacteria Isolated from Potable Water Stored in Household Tanks
title_sort Microbiological Quality and Genotypic Speciation of Heterotrophic Bacteria Isolated from Potable Water Stored in Household Tanks
url http://hdl.handle.net/10725/3399
https://www.cawq.ca/journal/temp/journal/3.pdf#page=70