Hypoxia silences retrotrapezoid nucleus respiratory chemoreceptors via alkalosis
In conscious mammals, hypoxia or hypercapnia stimulates breathing while theoretically exerting opposite effects on central respiratory chemoreceptors (CRCs). We tested this theory by examining how hypoxia and hypercapnia change the activity of the retrotrapezoid nucleus (RTN), a putative CRC and che...
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| مؤلفون آخرون: | , , , , , |
| التنسيق: | article |
| منشور في: |
2015
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| الوصول للمادة أونلاين: | http://hdl.handle.net/10725/4564 http://dx.doi.org/10.1523/JNEUROSCI.2923-14.2015 http://libraries.lau.edu.lb/research/laur/terms-of-use/articles.php https://www.jneurosci.org/content/35/2/527?utm_source=TrendMD&utm_medium=cpc&utm_campaign=JNeurosci_TrendMD_1 |
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| _version_ | 1864513464018403328 |
|---|---|
| author | Basting, Tyler M. |
| author2 | Burke, Peter G. R. Kanbar, Roy Viar, Kenneth E. Stornetta, Daniel S. Stornetta, Ruth L. Guyenet, Patrice G. |
| author2_role | author author author author author author |
| author_facet | Basting, Tyler M. Burke, Peter G. R. Kanbar, Roy Viar, Kenneth E. Stornetta, Daniel S. Stornetta, Ruth L. Guyenet, Patrice G. |
| author_role | author |
| dc.creator.none.fl_str_mv | Basting, Tyler M. Burke, Peter G. R. Kanbar, Roy Viar, Kenneth E. Stornetta, Daniel S. Stornetta, Ruth L. Guyenet, Patrice G. |
| dc.date.none.fl_str_mv | 2015 2016-10-11T10:01:39Z 2016-10-11T10:01:39Z 2016-10-11 |
| dc.identifier.none.fl_str_mv | 0270-6474 http://hdl.handle.net/10725/4564 http://dx.doi.org/10.1523/JNEUROSCI.2923-14.2015 Basting, T. M., Burke, P. G., Kanbar, R., Viar, K. E., Stornetta, D. S., Stornetta, R. L., & Guyenet, P. G. (2015). Hypoxia silences retrotrapezoid nucleus respiratory chemoreceptors via alkalosis. The Journal of Neuroscience, 35(2), 527-543. http://libraries.lau.edu.lb/research/laur/terms-of-use/articles.php https://www.jneurosci.org/content/35/2/527?utm_source=TrendMD&utm_medium=cpc&utm_campaign=JNeurosci_TrendMD_1 |
| dc.language.none.fl_str_mv | en |
| dc.relation.none.fl_str_mv | Journal of Neuroscience |
| dc.rights.*.fl_str_mv | info:eu-repo/semantics/openAccess |
| dc.title.none.fl_str_mv | Hypoxia silences retrotrapezoid nucleus respiratory chemoreceptors via alkalosis |
| dc.type.none.fl_str_mv | Article info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article |
| description | In conscious mammals, hypoxia or hypercapnia stimulates breathing while theoretically exerting opposite effects on central respiratory chemoreceptors (CRCs). We tested this theory by examining how hypoxia and hypercapnia change the activity of the retrotrapezoid nucleus (RTN), a putative CRC and chemoreflex integrator. Archaerhodopsin-(Arch)-transduced RTN neurons were reversibly silenced by light in anesthetized rats. We bilaterally transduced RTN and nearby C1 neurons with Arch (PRSx8-ArchT-EYFP-LVV) and measured the cardiorespiratory consequences of Arch activation (10 s) in conscious rats during normoxia, hypoxia, or hyperoxia. RTN photoinhibition reduced breathing equally during non-REM sleep and quiet wake. Compared with normoxia, the breathing frequency reduction (ΔfR) was larger in hyperoxia (65% FiO2), smaller in 15% FiO2, and absent in 12% FiO2. Tidal volume changes (ΔVT) followed the same trend. The effect of hypoxia on ΔfR was not arousal-dependent but was reversed by reacidifying the blood (acetazolamide; 3% FiCO2). ΔfR was highly correlated with arterial pH up to arterial pH (pHa) 7.5 with no frequency inhibition occurring above pHa 7.53. Blood pressure was minimally reduced suggesting that C1 neurons were very modestly inhibited. In conclusion, RTN neurons regulate eupneic breathing about equally during both sleep and wake. RTN neurons are the first putative CRCs demonstrably silenced by hypocapnic hypoxia in conscious mammals. RTN neurons are silent above pHa 7.5 and increasingly active below this value. During hyperoxia, RTN activation maintains breathing despite the inactivity of the carotid bodies. Finally, during hypocapnic hypoxia, carotid body stimulation increases breathing frequency via pathways that bypass RTN. |
| eu_rights_str_mv | openAccess |
| format | article |
| id | LAURepo_687c82ecc9a436ed042011e4cd1d3028 |
| identifier_str_mv | 0270-6474 Basting, T. M., Burke, P. G., Kanbar, R., Viar, K. E., Stornetta, D. S., Stornetta, R. L., & Guyenet, P. G. (2015). Hypoxia silences retrotrapezoid nucleus respiratory chemoreceptors via alkalosis. The Journal of Neuroscience, 35(2), 527-543. |
| language_invalid_str_mv | en |
| network_acronym_str | LAURepo |
| network_name_str | Lebanese American University repository |
| oai_identifier_str | oai:laur.lau.edu.lb:10725/4564 |
| publishDate | 2015 |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| spelling | Hypoxia silences retrotrapezoid nucleus respiratory chemoreceptors via alkalosisBasting, Tyler M.Burke, Peter G. R.Kanbar, RoyViar, Kenneth E.Stornetta, Daniel S.Stornetta, Ruth L.Guyenet, Patrice G.In conscious mammals, hypoxia or hypercapnia stimulates breathing while theoretically exerting opposite effects on central respiratory chemoreceptors (CRCs). We tested this theory by examining how hypoxia and hypercapnia change the activity of the retrotrapezoid nucleus (RTN), a putative CRC and chemoreflex integrator. Archaerhodopsin-(Arch)-transduced RTN neurons were reversibly silenced by light in anesthetized rats. We bilaterally transduced RTN and nearby C1 neurons with Arch (PRSx8-ArchT-EYFP-LVV) and measured the cardiorespiratory consequences of Arch activation (10 s) in conscious rats during normoxia, hypoxia, or hyperoxia. RTN photoinhibition reduced breathing equally during non-REM sleep and quiet wake. Compared with normoxia, the breathing frequency reduction (ΔfR) was larger in hyperoxia (65% FiO2), smaller in 15% FiO2, and absent in 12% FiO2. Tidal volume changes (ΔVT) followed the same trend. The effect of hypoxia on ΔfR was not arousal-dependent but was reversed by reacidifying the blood (acetazolamide; 3% FiCO2). ΔfR was highly correlated with arterial pH up to arterial pH (pHa) 7.5 with no frequency inhibition occurring above pHa 7.53. Blood pressure was minimally reduced suggesting that C1 neurons were very modestly inhibited. In conclusion, RTN neurons regulate eupneic breathing about equally during both sleep and wake. RTN neurons are the first putative CRCs demonstrably silenced by hypocapnic hypoxia in conscious mammals. RTN neurons are silent above pHa 7.5 and increasingly active below this value. During hyperoxia, RTN activation maintains breathing despite the inactivity of the carotid bodies. Finally, during hypocapnic hypoxia, carotid body stimulation increases breathing frequency via pathways that bypass RTN.PublishedN/A2016-10-11T10:01:39Z2016-10-11T10:01:39Z20152016-10-11Articleinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article0270-6474http://hdl.handle.net/10725/4564http://dx.doi.org/10.1523/JNEUROSCI.2923-14.2015Basting, T. M., Burke, P. G., Kanbar, R., Viar, K. E., Stornetta, D. S., Stornetta, R. L., & Guyenet, P. G. (2015). Hypoxia silences retrotrapezoid nucleus respiratory chemoreceptors via alkalosis. The Journal of Neuroscience, 35(2), 527-543.http://libraries.lau.edu.lb/research/laur/terms-of-use/articles.phphttps://www.jneurosci.org/content/35/2/527?utm_source=TrendMD&utm_medium=cpc&utm_campaign=JNeurosci_TrendMD_1enJournal of Neuroscienceinfo:eu-repo/semantics/openAccessoai:laur.lau.edu.lb:10725/45642021-03-19T10:03:21Z |
| spellingShingle | Hypoxia silences retrotrapezoid nucleus respiratory chemoreceptors via alkalosis Basting, Tyler M. |
| status_str | publishedVersion |
| title | Hypoxia silences retrotrapezoid nucleus respiratory chemoreceptors via alkalosis |
| title_full | Hypoxia silences retrotrapezoid nucleus respiratory chemoreceptors via alkalosis |
| title_fullStr | Hypoxia silences retrotrapezoid nucleus respiratory chemoreceptors via alkalosis |
| title_full_unstemmed | Hypoxia silences retrotrapezoid nucleus respiratory chemoreceptors via alkalosis |
| title_short | Hypoxia silences retrotrapezoid nucleus respiratory chemoreceptors via alkalosis |
| title_sort | Hypoxia silences retrotrapezoid nucleus respiratory chemoreceptors via alkalosis |
| url | http://hdl.handle.net/10725/4564 http://dx.doi.org/10.1523/JNEUROSCI.2923-14.2015 http://libraries.lau.edu.lb/research/laur/terms-of-use/articles.php https://www.jneurosci.org/content/35/2/527?utm_source=TrendMD&utm_medium=cpc&utm_campaign=JNeurosci_TrendMD_1 |