Daucus carota Pentane-Based Fractions Suppress Proliferation and Induce Apoptosis in Human Colon Adenocarcinoma HT-29 Cells by Inhibiting the MAPK and PI3K Pathways
Daucus carota L. ssp. carota (Apiacea, wild carrot, Queen Anne's lace) has been used in folk medicine throughout the world and recently was shown to possess anticancer and antioxidant activities. This study aims to determine the anticancer activity of the pentane fraction (F1) and the 1:1 penta...
محفوظ في:
| المؤلف الرئيسي: | |
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| مؤلفون آخرون: | , , , , , |
| التنسيق: | article |
| منشور في: |
2015
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| الوصول للمادة أونلاين: | http://hdl.handle.net/10725/2828 https://doi.org/10.1089/jmf.2014.3225 http://online.liebertpub.com/doi/abs/10.1089/jmf.2014.3225 |
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إضافة وسم
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| _version_ | 1864513459441369088 |
|---|---|
| author | Shehaby, Wassim N. |
| author2 | Bodman-Smith, K.B. Mansour, Anthony Mroueh, Mohamad Taleb, Robin I. El-Sibai, Mirvat Daher, Costantine F. |
| author2_role | author author author author author author |
| author_facet | Shehaby, Wassim N. Bodman-Smith, K.B. Mansour, Anthony Mroueh, Mohamad Taleb, Robin I. El-Sibai, Mirvat Daher, Costantine F. |
| author_role | author |
| dc.creator.none.fl_str_mv | Shehaby, Wassim N. Bodman-Smith, K.B. Mansour, Anthony Mroueh, Mohamad Taleb, Robin I. El-Sibai, Mirvat Daher, Costantine F. |
| dc.date.none.fl_str_mv | 2015-12-17T13:58:16Z 2015-12-17T13:58:16Z 2015 2015-12-17 |
| dc.identifier.none.fl_str_mv | 1096-620X http://hdl.handle.net/10725/2828 https://doi.org/10.1089/jmf.2014.3225 Shebaby, W. N., Bodman-Smith, K. B., Mansour, A., Mroueh, M., Taleb, R. I., El-Sibai, M., & Daher, C. F. (2015). Daucus carota pentane-based fractions suppress proliferation and induce apoptosis in human colon adenocarcinoma HT-29 cells by inhibiting the MAPK and PI3K pathways. Journal of Medicinal Food, 18(7), 745-752. http://online.liebertpub.com/doi/abs/10.1089/jmf.2014.3225 |
| dc.language.none.fl_str_mv | en |
| dc.relation.none.fl_str_mv | Journal of Medical Food |
| dc.rights.*.fl_str_mv | info:eu-repo/semantics/openAccess |
| dc.title.none.fl_str_mv | Daucus carota Pentane-Based Fractions Suppress Proliferation and Induce Apoptosis in Human Colon Adenocarcinoma HT-29 Cells by Inhibiting the MAPK and PI3K Pathways |
| dc.type.none.fl_str_mv | Article info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article |
| description | Daucus carota L. ssp. carota (Apiacea, wild carrot, Queen Anne's lace) has been used in folk medicine throughout the world and recently was shown to possess anticancer and antioxidant activities. This study aims to determine the anticancer activity of the pentane fraction (F1) and the 1:1 pentane:diethyl ether fraction (F2) of the Daucus Carota oil extract (DCOE) against human colon adenocarcinoma cell lines (HT-29 and Caco-2). Treatment of cells with various concentrations of F1 or F2 fractions produced a dose-dependent inhibition of cell proliferation. Flow cytometric analysis indicated that both fractions induced sub-G1 phase accumulation and increased apoptotic cell death. Western blot revealed the activation of caspase-3, PARP cleavage, and a considerable increase in Bax and p53 levels, and a decrease in Bcl-2 level. Treatment of HT-29 cells with either fraction markedly decreased the levels of both phosphorylated Erk and Akt. Furthermore, the combined treatment of F1 or F2 with wortmannin showed no added inhibition of cell survival suggesting an effect of F1 or F2 through the phosphatidyl inositol 3-kinase (PI3K) pathway. This study proposes that DCOE fractions (F1 and F2) inhibit cell proliferation by inducing cell cycle arrest and apoptosis in HT-29 cells through the suppression of mitogen-activated protein kinase (MAPK)/Erk and PI3K/Akt pathways. |
| eu_rights_str_mv | openAccess |
| format | article |
| id | LAURepo_6908ddafa951c528ba64d8e8fd258153 |
| identifier_str_mv | 1096-620X Shebaby, W. N., Bodman-Smith, K. B., Mansour, A., Mroueh, M., Taleb, R. I., El-Sibai, M., & Daher, C. F. (2015). Daucus carota pentane-based fractions suppress proliferation and induce apoptosis in human colon adenocarcinoma HT-29 cells by inhibiting the MAPK and PI3K pathways. Journal of Medicinal Food, 18(7), 745-752. |
| language_invalid_str_mv | en |
| network_acronym_str | LAURepo |
| network_name_str | Lebanese American University repository |
| oai_identifier_str | oai:laur.lau.edu.lb:10725/2828 |
| publishDate | 2015 |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| spelling | Daucus carota Pentane-Based Fractions Suppress Proliferation and Induce Apoptosis in Human Colon Adenocarcinoma HT-29 Cells by Inhibiting the MAPK and PI3K PathwaysShehaby, Wassim N.Bodman-Smith, K.B.Mansour, AnthonyMroueh, MohamadTaleb, Robin I.El-Sibai, MirvatDaher, Costantine F.Daucus carota L. ssp. carota (Apiacea, wild carrot, Queen Anne's lace) has been used in folk medicine throughout the world and recently was shown to possess anticancer and antioxidant activities. This study aims to determine the anticancer activity of the pentane fraction (F1) and the 1:1 pentane:diethyl ether fraction (F2) of the Daucus Carota oil extract (DCOE) against human colon adenocarcinoma cell lines (HT-29 and Caco-2). Treatment of cells with various concentrations of F1 or F2 fractions produced a dose-dependent inhibition of cell proliferation. Flow cytometric analysis indicated that both fractions induced sub-G1 phase accumulation and increased apoptotic cell death. Western blot revealed the activation of caspase-3, PARP cleavage, and a considerable increase in Bax and p53 levels, and a decrease in Bcl-2 level. Treatment of HT-29 cells with either fraction markedly decreased the levels of both phosphorylated Erk and Akt. Furthermore, the combined treatment of F1 or F2 with wortmannin showed no added inhibition of cell survival suggesting an effect of F1 or F2 through the phosphatidyl inositol 3-kinase (PI3K) pathway. This study proposes that DCOE fractions (F1 and F2) inhibit cell proliferation by inducing cell cycle arrest and apoptosis in HT-29 cells through the suppression of mitogen-activated protein kinase (MAPK)/Erk and PI3K/Akt pathways.PublishedN/A2015-12-17T13:58:16Z2015-12-17T13:58:16Z20152015-12-17Articleinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1096-620Xhttp://hdl.handle.net/10725/2828https://doi.org/10.1089/jmf.2014.3225Shebaby, W. N., Bodman-Smith, K. B., Mansour, A., Mroueh, M., Taleb, R. I., El-Sibai, M., & Daher, C. F. (2015). Daucus carota pentane-based fractions suppress proliferation and induce apoptosis in human colon adenocarcinoma HT-29 cells by inhibiting the MAPK and PI3K pathways. Journal of Medicinal Food, 18(7), 745-752.http://online.liebertpub.com/doi/abs/10.1089/jmf.2014.3225enJournal of Medical Foodinfo:eu-repo/semantics/openAccessoai:laur.lau.edu.lb:10725/28282024-01-31T14:57:42Z |
| spellingShingle | Daucus carota Pentane-Based Fractions Suppress Proliferation and Induce Apoptosis in Human Colon Adenocarcinoma HT-29 Cells by Inhibiting the MAPK and PI3K Pathways Shehaby, Wassim N. |
| status_str | publishedVersion |
| title | Daucus carota Pentane-Based Fractions Suppress Proliferation and Induce Apoptosis in Human Colon Adenocarcinoma HT-29 Cells by Inhibiting the MAPK and PI3K Pathways |
| title_full | Daucus carota Pentane-Based Fractions Suppress Proliferation and Induce Apoptosis in Human Colon Adenocarcinoma HT-29 Cells by Inhibiting the MAPK and PI3K Pathways |
| title_fullStr | Daucus carota Pentane-Based Fractions Suppress Proliferation and Induce Apoptosis in Human Colon Adenocarcinoma HT-29 Cells by Inhibiting the MAPK and PI3K Pathways |
| title_full_unstemmed | Daucus carota Pentane-Based Fractions Suppress Proliferation and Induce Apoptosis in Human Colon Adenocarcinoma HT-29 Cells by Inhibiting the MAPK and PI3K Pathways |
| title_short | Daucus carota Pentane-Based Fractions Suppress Proliferation and Induce Apoptosis in Human Colon Adenocarcinoma HT-29 Cells by Inhibiting the MAPK and PI3K Pathways |
| title_sort | Daucus carota Pentane-Based Fractions Suppress Proliferation and Induce Apoptosis in Human Colon Adenocarcinoma HT-29 Cells by Inhibiting the MAPK and PI3K Pathways |
| url | http://hdl.handle.net/10725/2828 https://doi.org/10.1089/jmf.2014.3225 http://online.liebertpub.com/doi/abs/10.1089/jmf.2014.3225 |