Annona cherimola Seed Extract Activates Extrinsic and Intrinsic Apoptotic Pathways in Leukemic Cells
Annona cherimola Mill is a large green fruit with black seeds widely known to possess toxic properties due to the presence of Annonaceous acetogenins. The present study investigates the anti-cancer properties of an Annona cherimola Mill ethanolic seed extract on Acute Myeloid Leukemia (AML) cell lin...
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| Format: | article |
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2019
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| Online Access: | http://hdl.handle.net/10725/11595 https://doi.org/10.3390/toxins11090506 http://libraries.lau.edu.lb/research/laur/terms-of-use/articles.php https://www.mdpi.com/2072-6651/11/9/506 |
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| _version_ | 1864513489252384768 |
|---|---|
| author | Haykal, Tony |
| author2 | Nasr, Peter Hodroj, Mohammad H. Taleb, Robin I. Sarkis, Rita El Moujabber, Marvy Nadine Rizk, Sandra |
| author2_role | author author author author author author |
| author_facet | Haykal, Tony Nasr, Peter Hodroj, Mohammad H. Taleb, Robin I. Sarkis, Rita El Moujabber, Marvy Nadine Rizk, Sandra |
| author_role | author |
| dc.creator.none.fl_str_mv | Haykal, Tony Nasr, Peter Hodroj, Mohammad H. Taleb, Robin I. Sarkis, Rita El Moujabber, Marvy Nadine Rizk, Sandra |
| dc.date.none.fl_str_mv | 2019-12-03T10:14:10Z 2019-12-03T10:14:10Z 2019 2019-12-03 |
| dc.identifier.none.fl_str_mv | 2072-6651 http://hdl.handle.net/10725/11595 https://doi.org/10.3390/toxins11090506 Haykal, T., Nasr, P., Hodroj, M. H., Taleb, R. I., Sarkis, R., Moujabber, M. N. E., & Rizk, S. (2019). Annona cherimola Seed Extract Activates Extrinsic and Intrinsic Apoptotic Pathways in Leukemic Cells. Toxins, 11(9), 1-18. http://libraries.lau.edu.lb/research/laur/terms-of-use/articles.php https://www.mdpi.com/2072-6651/11/9/506 |
| dc.language.none.fl_str_mv | en |
| dc.relation.none.fl_str_mv | Toxins |
| dc.rights.*.fl_str_mv | info:eu-repo/semantics/openAccess |
| dc.title.none.fl_str_mv | Annona cherimola Seed Extract Activates Extrinsic and Intrinsic Apoptotic Pathways in Leukemic Cells |
| dc.type.none.fl_str_mv | Article info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article |
| description | Annona cherimola Mill is a large green fruit with black seeds widely known to possess toxic properties due to the presence of Annonaceous acetogenins. The present study investigates the anti-cancer properties of an Annona cherimola Mill ethanolic seed extract on Acute Myeloid Leukemia (AML) cell lines in vitro and elucidates the underlying cellular mechanism. The anti-proliferative effects of the extract on various AML cell lines and normal mesenchymal cells (MSCs) were assessed using WST-1 viability reagent. The pro-apoptotic effect of the extract was evaluated using Annexin V/PI staining and Cell Death ELISA. The underlying mechanism was deciphered by analyzing the expression of various proteins using western blots. Treatment with an A. cherimola seed ethanolic extract promotes a dose- and time-dependent inhibition of the proliferation of various AML cell lines, but not MSCs. Positive Annexin V staining, as well as DNA fragmentation, confirm an increase in apoptotic cell death by upregulating the expression of pro-apoptotic proteins which control both intrinsic and extrinsic pathways of apoptosis. GC/MS analysis revealed the presence of phytosterols, in addition to other bioactive compounds. In conclusion, Annona cherimola Mill seed extract, previously known to possess a potent toxic activity, induces apoptosis in AML cell lines by the activation of both the extrinsic and the intrinsic pathways. |
| eu_rights_str_mv | openAccess |
| format | article |
| id | LAURepo_6a8a7e58b535bccca55189df78932149 |
| identifier_str_mv | 2072-6651 Haykal, T., Nasr, P., Hodroj, M. H., Taleb, R. I., Sarkis, R., Moujabber, M. N. E., & Rizk, S. (2019). Annona cherimola Seed Extract Activates Extrinsic and Intrinsic Apoptotic Pathways in Leukemic Cells. Toxins, 11(9), 1-18. |
| language_invalid_str_mv | en |
| network_acronym_str | LAURepo |
| network_name_str | Lebanese American University repository |
| oai_identifier_str | oai:laur.lau.edu.lb:10725/11595 |
| publishDate | 2019 |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| spelling | Annona cherimola Seed Extract Activates Extrinsic and Intrinsic Apoptotic Pathways in Leukemic CellsHaykal, TonyNasr, PeterHodroj, Mohammad H.Taleb, Robin I.Sarkis, RitaEl Moujabber, Marvy NadineRizk, SandraAnnona cherimola Mill is a large green fruit with black seeds widely known to possess toxic properties due to the presence of Annonaceous acetogenins. The present study investigates the anti-cancer properties of an Annona cherimola Mill ethanolic seed extract on Acute Myeloid Leukemia (AML) cell lines in vitro and elucidates the underlying cellular mechanism. The anti-proliferative effects of the extract on various AML cell lines and normal mesenchymal cells (MSCs) were assessed using WST-1 viability reagent. The pro-apoptotic effect of the extract was evaluated using Annexin V/PI staining and Cell Death ELISA. The underlying mechanism was deciphered by analyzing the expression of various proteins using western blots. Treatment with an A. cherimola seed ethanolic extract promotes a dose- and time-dependent inhibition of the proliferation of various AML cell lines, but not MSCs. Positive Annexin V staining, as well as DNA fragmentation, confirm an increase in apoptotic cell death by upregulating the expression of pro-apoptotic proteins which control both intrinsic and extrinsic pathways of apoptosis. GC/MS analysis revealed the presence of phytosterols, in addition to other bioactive compounds. In conclusion, Annona cherimola Mill seed extract, previously known to possess a potent toxic activity, induces apoptosis in AML cell lines by the activation of both the extrinsic and the intrinsic pathways.PublishedN/A2019-12-03T10:14:10Z2019-12-03T10:14:10Z20192019-12-03Articleinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article2072-6651http://hdl.handle.net/10725/11595https://doi.org/10.3390/toxins11090506Haykal, T., Nasr, P., Hodroj, M. H., Taleb, R. I., Sarkis, R., Moujabber, M. N. E., & Rizk, S. (2019). Annona cherimola Seed Extract Activates Extrinsic and Intrinsic Apoptotic Pathways in Leukemic Cells. Toxins, 11(9), 1-18.http://libraries.lau.edu.lb/research/laur/terms-of-use/articles.phphttps://www.mdpi.com/2072-6651/11/9/506enToxinsinfo:eu-repo/semantics/openAccessoai:laur.lau.edu.lb:10725/115952021-03-19T10:47:39Z |
| spellingShingle | Annona cherimola Seed Extract Activates Extrinsic and Intrinsic Apoptotic Pathways in Leukemic Cells Haykal, Tony |
| status_str | publishedVersion |
| title | Annona cherimola Seed Extract Activates Extrinsic and Intrinsic Apoptotic Pathways in Leukemic Cells |
| title_full | Annona cherimola Seed Extract Activates Extrinsic and Intrinsic Apoptotic Pathways in Leukemic Cells |
| title_fullStr | Annona cherimola Seed Extract Activates Extrinsic and Intrinsic Apoptotic Pathways in Leukemic Cells |
| title_full_unstemmed | Annona cherimola Seed Extract Activates Extrinsic and Intrinsic Apoptotic Pathways in Leukemic Cells |
| title_short | Annona cherimola Seed Extract Activates Extrinsic and Intrinsic Apoptotic Pathways in Leukemic Cells |
| title_sort | Annona cherimola Seed Extract Activates Extrinsic and Intrinsic Apoptotic Pathways in Leukemic Cells |
| url | http://hdl.handle.net/10725/11595 https://doi.org/10.3390/toxins11090506 http://libraries.lau.edu.lb/research/laur/terms-of-use/articles.php https://www.mdpi.com/2072-6651/11/9/506 |