Buccal Cell DNA Extraction
Simple and cost-effective methods are needed to extract DNA in order to use it in large-scale studies. Blood is an excellent DNA source; however, it is costly and invasive thus an alternative is needed. Several kits and chemical protocols using buccal cells have been proposed for DNA extraction. The...
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2008
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| Online Access: | http://hdl.handle.net/10725/4382 http://dx.doi.org/10.1089/gte.2007.0044 http://libraries.lau.edu.lb/research/laur/terms-of-use/articles.php http://online.liebertpub.com/doi/abs/10.1089/gte.2007.0044 |
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| _version_ | 1864513463282302976 |
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| author | Saab, Yolande B. |
| author2 | Kabbara, Wissam Chebib, Christiane Gard, Paul Richard |
| author2_role | author author author |
| author_facet | Saab, Yolande B. Kabbara, Wissam Chebib, Christiane Gard, Paul Richard |
| author_role | author |
| dc.creator.none.fl_str_mv | Saab, Yolande B. Kabbara, Wissam Chebib, Christiane Gard, Paul Richard |
| dc.date.none.fl_str_mv | 2008 2016-09-26T08:45:38Z 2016-09-26T08:45:38Z 2016-09-26 |
| dc.identifier.none.fl_str_mv | 1090-6576 http://hdl.handle.net/10725/4382 http://dx.doi.org/10.1089/gte.2007.0044 Saab, Y. B., Kabbara, W., Chbib, C., & Gard, P. R. (2007). Buccal cell DNA extraction: yield, purity, and cost: a comparison of two methods. Genetic Testing, 11(4), 413-416. http://libraries.lau.edu.lb/research/laur/terms-of-use/articles.php http://online.liebertpub.com/doi/abs/10.1089/gte.2007.0044 |
| dc.language.none.fl_str_mv | en |
| dc.relation.none.fl_str_mv | Genetic Testing |
| dc.rights.*.fl_str_mv | info:eu-repo/semantics/openAccess |
| dc.title.none.fl_str_mv | Buccal Cell DNA Extraction yield, purity, and cost: a comparison of two methods |
| dc.type.none.fl_str_mv | Article info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article |
| description | Simple and cost-effective methods are needed to extract DNA in order to use it in large-scale studies. Blood is an excellent DNA source; however, it is costly and invasive thus an alternative is needed. Several kits and chemical protocols using buccal cells have been proposed for DNA extraction. The objective of the study is to evaluate buccal NaOH chemical protocol and Nucleospin® Tissue Kit (BD Biosciences, Macery-Nagel, Germany) for DNA extraction. DNA swab samples were collected from 300 voluntary participants. DNA yields and purity were measured by NaOH and Nucleospin® Tissue Kit techniques; the cost and time consumption for DNA extraction per sample were assessed as well. Results have shown that DNA amount and purity extracted by NaOH procedure was compared to that of the kit (p = 0.164; p = 0.249, respectively). NaOH method was considered cheaper and less time consuming (0.06 versus 3.80 USD, and 1.33 versus 3.59 minutes per sample, p < 0.001). Buccal cell derived DNA extracted by NaOH protocol can be considered a feasible substitute for more expensive and time-consuming kits. |
| eu_rights_str_mv | openAccess |
| format | article |
| id | LAURepo_6bf80ae97e047dcf007d456e48cf6c23 |
| identifier_str_mv | 1090-6576 Saab, Y. B., Kabbara, W., Chbib, C., & Gard, P. R. (2007). Buccal cell DNA extraction: yield, purity, and cost: a comparison of two methods. Genetic Testing, 11(4), 413-416. |
| language_invalid_str_mv | en |
| network_acronym_str | LAURepo |
| network_name_str | Lebanese American University repository |
| oai_identifier_str | oai:laur.lau.edu.lb:10725/4382 |
| publishDate | 2008 |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| spelling | Buccal Cell DNA Extractionyield, purity, and cost: a comparison of two methodsSaab, Yolande B.Kabbara, WissamChebib, ChristianeGard, Paul RichardSimple and cost-effective methods are needed to extract DNA in order to use it in large-scale studies. Blood is an excellent DNA source; however, it is costly and invasive thus an alternative is needed. Several kits and chemical protocols using buccal cells have been proposed for DNA extraction. The objective of the study is to evaluate buccal NaOH chemical protocol and Nucleospin® Tissue Kit (BD Biosciences, Macery-Nagel, Germany) for DNA extraction. DNA swab samples were collected from 300 voluntary participants. DNA yields and purity were measured by NaOH and Nucleospin® Tissue Kit techniques; the cost and time consumption for DNA extraction per sample were assessed as well. Results have shown that DNA amount and purity extracted by NaOH procedure was compared to that of the kit (p = 0.164; p = 0.249, respectively). NaOH method was considered cheaper and less time consuming (0.06 versus 3.80 USD, and 1.33 versus 3.59 minutes per sample, p < 0.001). Buccal cell derived DNA extracted by NaOH protocol can be considered a feasible substitute for more expensive and time-consuming kits.PublishedN/A2016-09-26T08:45:38Z2016-09-26T08:45:38Z20082016-09-26Articleinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1090-6576http://hdl.handle.net/10725/4382http://dx.doi.org/10.1089/gte.2007.0044Saab, Y. B., Kabbara, W., Chbib, C., & Gard, P. R. (2007). Buccal cell DNA extraction: yield, purity, and cost: a comparison of two methods. Genetic Testing, 11(4), 413-416.http://libraries.lau.edu.lb/research/laur/terms-of-use/articles.phphttp://online.liebertpub.com/doi/abs/10.1089/gte.2007.0044enGenetic Testinginfo:eu-repo/semantics/openAccessoai:laur.lau.edu.lb:10725/43822021-03-19T10:03:17Z |
| spellingShingle | Buccal Cell DNA Extraction Saab, Yolande B. |
| status_str | publishedVersion |
| title | Buccal Cell DNA Extraction |
| title_full | Buccal Cell DNA Extraction |
| title_fullStr | Buccal Cell DNA Extraction |
| title_full_unstemmed | Buccal Cell DNA Extraction |
| title_short | Buccal Cell DNA Extraction |
| title_sort | Buccal Cell DNA Extraction |
| url | http://hdl.handle.net/10725/4382 http://dx.doi.org/10.1089/gte.2007.0044 http://libraries.lau.edu.lb/research/laur/terms-of-use/articles.php http://online.liebertpub.com/doi/abs/10.1089/gte.2007.0044 |