STARD 13 in prostate cancer. (c2018)

Prostate cancer is the most commonly diagnosed solid organ tumor and one of the main leading causes of death – due to cancerous tumors- among men in the world. Malignancy of a tumor is characterized by the rapid and uncontrolled growth and its ability to metastasize. Proliferation and migration are...

وصف كامل

محفوظ في:
التفاصيل البيبلوغرافية
المؤلف الرئيسي: Saab, Sahar S. (author)
التنسيق: masterThesis
منشور في: 2018
الموضوعات:
الوصول للمادة أونلاين:http://hdl.handle.net/10725/8529
https://doi.org/10.26756/th.2018.62
http://libraries.lau.edu.lb/research/laur/terms-of-use/thesis.php
الوسوم: إضافة وسم
لا توجد وسوم, كن أول من يضع وسما على هذه التسجيلة!
_version_ 1864513485016137728
author Saab, Sahar S.
author_facet Saab, Sahar S.
author_role author
dc.creator.none.fl_str_mv Saab, Sahar S.
dc.date.none.fl_str_mv 2018-10-01T09:24:18Z
2018-10-01T09:24:18Z
2018
2018-10-01
2018-05-14
dc.identifier.none.fl_str_mv http://hdl.handle.net/10725/8529
https://doi.org/10.26756/th.2018.62
http://libraries.lau.edu.lb/research/laur/terms-of-use/thesis.php
dc.language.none.fl_str_mv en
dc.publisher.none.fl_str_mv Lebanese American University
dc.rights.*.fl_str_mv info:eu-repo/semantics/openAccess
dc.subject.none.fl_str_mv Prostate -- Cancer -- Research
Cancer cells -- Growth -- Regulation
Antioncogenes
GTPase-activating protein
dc.title.none.fl_str_mv STARD 13 in prostate cancer. (c2018)
a shining star or an oncogene?
dc.type.none.fl_str_mv Thesis
info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/masterThesis
description Prostate cancer is the most commonly diagnosed solid organ tumor and one of the main leading causes of death – due to cancerous tumors- among men in the world. Malignancy of a tumor is characterized by the rapid and uncontrolled growth and its ability to metastasize. Proliferation and migration are known to be regulated by Rho family of GTPases. StarD13 is a GAP for Rho GTPases and has been previously described to be a tumor suppressor in astrocytoma, breast and colorectal cancer. In this study, we investigate the role of StarD13, an inhibitor of RhoA and Cdc42 through its GAP activity, on prostate cancer cells proliferation and metastasis by manipulating its level of expression in the cells. Knocking down StarD13 by siRNA led to an increase in cell proliferation and adhesion yet a decrease in cell motility and cell speed in prostate cancer. The activation levels of CDC42 following StarD13 knockdown are also being investigated through FRET. Our data showed that StarD13 knockdown had no significant effect on prostate cancer cell invasion; further investigations are to be accomplished in order to discover the reasons behind this insignificance.
eu_rights_str_mv openAccess
format masterThesis
id LAURepo_6d5ab254a3322fcfdc07bf12d36657a7
language_invalid_str_mv en
network_acronym_str LAURepo
network_name_str Lebanese American University repository
oai_identifier_str oai:laur.lau.edu.lb:10725/8529
publishDate 2018
publisher.none.fl_str_mv Lebanese American University
repository.mail.fl_str_mv
repository.name.fl_str_mv
repository_id_str
spelling STARD 13 in prostate cancer. (c2018)a shining star or an oncogene?Saab, Sahar S.Prostate -- Cancer -- ResearchCancer cells -- Growth -- RegulationAntioncogenesGTPase-activating proteinProstate cancer is the most commonly diagnosed solid organ tumor and one of the main leading causes of death – due to cancerous tumors- among men in the world. Malignancy of a tumor is characterized by the rapid and uncontrolled growth and its ability to metastasize. Proliferation and migration are known to be regulated by Rho family of GTPases. StarD13 is a GAP for Rho GTPases and has been previously described to be a tumor suppressor in astrocytoma, breast and colorectal cancer. In this study, we investigate the role of StarD13, an inhibitor of RhoA and Cdc42 through its GAP activity, on prostate cancer cells proliferation and metastasis by manipulating its level of expression in the cells. Knocking down StarD13 by siRNA led to an increase in cell proliferation and adhesion yet a decrease in cell motility and cell speed in prostate cancer. The activation levels of CDC42 following StarD13 knockdown are also being investigated through FRET. Our data showed that StarD13 knockdown had no significant effect on prostate cancer cell invasion; further investigations are to be accomplished in order to discover the reasons behind this insignificance.N/A1 hard copy: xiii, 38 leaves; col. ill.; 30 cm. available at RNL.Bibliography : leaves 35-38.Lebanese American University2018-10-01T09:24:18Z2018-10-01T09:24:18Z20182018-10-012018-05-14Thesisinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesishttp://hdl.handle.net/10725/8529https://doi.org/10.26756/th.2018.62http://libraries.lau.edu.lb/research/laur/terms-of-use/thesis.phpeninfo:eu-repo/semantics/openAccessoai:laur.lau.edu.lb:10725/85292021-03-19T10:45:17Z
spellingShingle STARD 13 in prostate cancer. (c2018)
Saab, Sahar S.
Prostate -- Cancer -- Research
Cancer cells -- Growth -- Regulation
Antioncogenes
GTPase-activating protein
status_str publishedVersion
title STARD 13 in prostate cancer. (c2018)
title_full STARD 13 in prostate cancer. (c2018)
title_fullStr STARD 13 in prostate cancer. (c2018)
title_full_unstemmed STARD 13 in prostate cancer. (c2018)
title_short STARD 13 in prostate cancer. (c2018)
title_sort STARD 13 in prostate cancer. (c2018)
topic Prostate -- Cancer -- Research
Cancer cells -- Growth -- Regulation
Antioncogenes
GTPase-activating protein
url http://hdl.handle.net/10725/8529
https://doi.org/10.26756/th.2018.62
http://libraries.lau.edu.lb/research/laur/terms-of-use/thesis.php