Autophagy Mediates the Effects of Physical Exercise on Learning and Memory through Activation of Hippocampal BDNF
Physical exercise is known to enhance learning and memory formation in the brain. These positive outcomes are mediated through the induction of the expression of a growth factor, brain derived neurotrophic factor (BDNF), in the hippocampus. BDNF promotes cognitive behaviors and induces brain plastic...
محفوظ في:
| المؤلف الرئيسي: | |
|---|---|
| التنسيق: | masterThesis |
| منشور في: |
2020
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| الموضوعات: | |
| الوصول للمادة أونلاين: | http://hdl.handle.net/10725/13485 https://doi.org/10.26756/th.2022.302 http://libraries.lau.edu.lb/research/laur/terms-of-use/thesis.php |
| الوسوم: |
إضافة وسم
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| _version_ | 1864513490768625664 |
|---|---|
| author | Jabr, Vanessa |
| author_facet | Jabr, Vanessa |
| author_role | author |
| dc.creator.none.fl_str_mv | Jabr, Vanessa |
| dc.date.none.fl_str_mv | 2020 2020-07-30 2022-04-12T11:45:01Z 2022-04-12T11:45:01Z |
| dc.identifier.none.fl_str_mv | http://hdl.handle.net/10725/13485 https://doi.org/10.26756/th.2022.302 http://libraries.lau.edu.lb/research/laur/terms-of-use/thesis.php |
| dc.language.none.fl_str_mv | en |
| dc.publisher.none.fl_str_mv | Lebanese American University |
| dc.rights.*.fl_str_mv | info:eu-repo/semantics/openAccess |
| dc.subject.none.fl_str_mv | Hippocampus (Brain) -- Physiology Memory -- Physiological aspects Learning -- Physiological aspects Autophagic vacuoles -- Therapeutic use Exercise -- Physiological aspects Lebanese American University -- Dissertations Dissertations, Academic |
| dc.title.none.fl_str_mv | Autophagy Mediates the Effects of Physical Exercise on Learning and Memory through Activation of Hippocampal BDNF |
| dc.type.none.fl_str_mv | Thesis info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/masterThesis |
| description | Physical exercise is known to enhance learning and memory formation in the brain. These positive outcomes are mediated through the induction of the expression of a growth factor, brain derived neurotrophic factor (BDNF), in the hippocampus. BDNF promotes cognitive behaviors and induces brain plasticity. Previous work has also reported that stimulating autophagy can restore cognitive ability. In this study, we demonstrated that a short-term voluntary exercise paradigm is sufficient to upregulate autophagy in different brain regions in an age-dependent manner. This increase in autophagy was correlated with enhanced spatial learning and memory formation particularly in mature adult mice (10-week-old). Indeed, we showed that short-term voluntary wheel running increases the protein expression levels of the autophagy marker, protein light chain 3 (LC3B), in the hippocampus of 10-week-old mice. We used the Morris Water Maze to evaluate spatial learning and memory performance in mice belonging to different age groups. Our work revealed that 10-week-old exercising mice that were treated with a brain-permeable autophagy inhibitor during the behavioral test showed cognitive deficits in the maze suggesting that induction of autophagy is necessary for exercise-induced learning and memory formation. Interestingly, we also found that inhibition of autophagy in exercising 10-week-old mice decreases BDNF protein levels in the hippocampus as compared to the control exercising group. Overall, our results suggest that BDNF acts downstream of exercise-induced autophagy to promote learning and memory formation in mature adult mice. We found that this pathway is not conserved in juvenile or middle-aged mice. |
| eu_rights_str_mv | openAccess |
| format | masterThesis |
| id | LAURepo_75cef68db5295bb043c14557a6f52db6 |
| language_invalid_str_mv | en |
| network_acronym_str | LAURepo |
| network_name_str | Lebanese American University repository |
| oai_identifier_str | oai:laur.lau.edu.lb:10725/13485 |
| publishDate | 2020 |
| publisher.none.fl_str_mv | Lebanese American University |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| spelling | Autophagy Mediates the Effects of Physical Exercise on Learning and Memory through Activation of Hippocampal BDNFJabr, VanessaHippocampus (Brain) -- PhysiologyMemory -- Physiological aspectsLearning -- Physiological aspectsAutophagic vacuoles -- Therapeutic useExercise -- Physiological aspectsLebanese American University -- DissertationsDissertations, AcademicPhysical exercise is known to enhance learning and memory formation in the brain. These positive outcomes are mediated through the induction of the expression of a growth factor, brain derived neurotrophic factor (BDNF), in the hippocampus. BDNF promotes cognitive behaviors and induces brain plasticity. Previous work has also reported that stimulating autophagy can restore cognitive ability. In this study, we demonstrated that a short-term voluntary exercise paradigm is sufficient to upregulate autophagy in different brain regions in an age-dependent manner. This increase in autophagy was correlated with enhanced spatial learning and memory formation particularly in mature adult mice (10-week-old). Indeed, we showed that short-term voluntary wheel running increases the protein expression levels of the autophagy marker, protein light chain 3 (LC3B), in the hippocampus of 10-week-old mice. We used the Morris Water Maze to evaluate spatial learning and memory performance in mice belonging to different age groups. Our work revealed that 10-week-old exercising mice that were treated with a brain-permeable autophagy inhibitor during the behavioral test showed cognitive deficits in the maze suggesting that induction of autophagy is necessary for exercise-induced learning and memory formation. Interestingly, we also found that inhibition of autophagy in exercising 10-week-old mice decreases BDNF protein levels in the hippocampus as compared to the control exercising group. Overall, our results suggest that BDNF acts downstream of exercise-induced autophagy to promote learning and memory formation in mature adult mice. We found that this pathway is not conserved in juvenile or middle-aged mice.1 online resource (ix, 45 leaves) : ill. (some col.)Includes bibliographical references (leaf 35-45).Lebanese American University2022-04-12T11:45:01Z2022-04-12T11:45:01Z20202020-07-30Thesisinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesishttp://hdl.handle.net/10725/13485https://doi.org/10.26756/th.2022.302http://libraries.lau.edu.lb/research/laur/terms-of-use/thesis.phpeninfo:eu-repo/semantics/openAccessoai:laur.lau.edu.lb:10725/134852022-07-13T08:12:43Z |
| spellingShingle | Autophagy Mediates the Effects of Physical Exercise on Learning and Memory through Activation of Hippocampal BDNF Jabr, Vanessa Hippocampus (Brain) -- Physiology Memory -- Physiological aspects Learning -- Physiological aspects Autophagic vacuoles -- Therapeutic use Exercise -- Physiological aspects Lebanese American University -- Dissertations Dissertations, Academic |
| status_str | publishedVersion |
| title | Autophagy Mediates the Effects of Physical Exercise on Learning and Memory through Activation of Hippocampal BDNF |
| title_full | Autophagy Mediates the Effects of Physical Exercise on Learning and Memory through Activation of Hippocampal BDNF |
| title_fullStr | Autophagy Mediates the Effects of Physical Exercise on Learning and Memory through Activation of Hippocampal BDNF |
| title_full_unstemmed | Autophagy Mediates the Effects of Physical Exercise on Learning and Memory through Activation of Hippocampal BDNF |
| title_short | Autophagy Mediates the Effects of Physical Exercise on Learning and Memory through Activation of Hippocampal BDNF |
| title_sort | Autophagy Mediates the Effects of Physical Exercise on Learning and Memory through Activation of Hippocampal BDNF |
| topic | Hippocampus (Brain) -- Physiology Memory -- Physiological aspects Learning -- Physiological aspects Autophagic vacuoles -- Therapeutic use Exercise -- Physiological aspects Lebanese American University -- Dissertations Dissertations, Academic |
| url | http://hdl.handle.net/10725/13485 https://doi.org/10.26756/th.2022.302 http://libraries.lau.edu.lb/research/laur/terms-of-use/thesis.php |