Chemopreventive effects of wild carrot oil against 7,12-dimethyl benz(a)anthracene-induced squamous cell carcinoma in mice
Context: Daucus carota L. ssp. carota (Apiacea) is widely distributed throughout the world and has many uses in traditional medicine. Objective: The present study investigates the chemopreventive effects of oil extract of D. carota umbels on 7,12-dimethyl benz(a)anthracene (DMBA)-induced skin cancer...
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2011
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| Online Access: | http://hdl.handle.net/10725/2745 http://dx.doi.org/10.3109/13880209.2011.559250 http://www.tandfonline.com/doi/abs/10.3109/13880209.2011.559250 |
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| _version_ | 1864513459141476352 |
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| author | Abu Zeinab, Rami |
| author2 | Mroueh, Mohamad Diab-Assaf, Mona Jurjus, Abdo Wex, Brigitte Sakr, Amer Daher, Costantine F. |
| author2_role | author author author author author author |
| author_facet | Abu Zeinab, Rami Mroueh, Mohamad Diab-Assaf, Mona Jurjus, Abdo Wex, Brigitte Sakr, Amer Daher, Costantine F. |
| author_role | author |
| dc.creator.none.fl_str_mv | Abu Zeinab, Rami Mroueh, Mohamad Diab-Assaf, Mona Jurjus, Abdo Wex, Brigitte Sakr, Amer Daher, Costantine F. |
| dc.date.none.fl_str_mv | 2011-07-21 2015-12-01T08:58:50Z 2015-12-01T08:58:50Z 2015-12-01 |
| dc.identifier.none.fl_str_mv | 1388-0209 http://hdl.handle.net/10725/2745 http://dx.doi.org/10.3109/13880209.2011.559250 Zeinab, R. A., Mroueh, M., Diab-Assaf, M., Jurjus, A., Wex, B., Sakr, A., & Daher, C. F. (2011). Chemopreventive effects of wild carrot oil against 7, 12-dimethyl benz (a) anthracene-induced squamous cell carcinoma in mice. Pharmaceutical biology, 49(9), 955-961. http://www.tandfonline.com/doi/abs/10.3109/13880209.2011.559250 |
| dc.language.none.fl_str_mv | en |
| dc.relation.none.fl_str_mv | Pharmaceutical Biology |
| dc.rights.*.fl_str_mv | info:eu-repo/semantics/openAccess |
| dc.title.none.fl_str_mv | Chemopreventive effects of wild carrot oil against 7,12-dimethyl benz(a)anthracene-induced squamous cell carcinoma in mice |
| dc.type.none.fl_str_mv | Article info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article |
| description | Context: Daucus carota L. ssp. carota (Apiacea) is widely distributed throughout the world and has many uses in traditional medicine. Objective: The present study investigates the chemopreventive effects of oil extract of D. carota umbels on 7,12-dimethyl benz(a)anthracene (DMBA)-induced skin cancer in mice. Materials and methods: D. carota oil extract (DCOE) was prepared by extracting the dried umbels with 50:50 acetone:methanol. Skin papilloma were initiated by DMBA and promoted by 12-O-tetradecanoyl phorobol-13-acetate (TPA). The extract was administered to animals via gavage (0.02 mL of 100% oil), intraperitoneal (0.3 mL of 2% oil), and topical (0.2 mL of 5, 50, and 100% oil) routes for 20 weeks. Tumor appearance, incidence, yield, and volume were compared with those of a non-treated control group. Results: Topical 100% treatment delayed tumor appearance, and inhibited tumor incidence and yield by 40 and 89%, respectively. Topical 50% treatment inhibited tumor incidence and yield by 30 and 83%, respectively, whereas the 5% treatment inhibited tumor yield by 36%. Tumor volume was decreased by 99, 91, and 70% following topical treatments with 100, 50, and 5% oil, respectively. Intraperitoneal treatment inhibited tumor yield by 43%, and decreased tumor volume by 85%, whereas gavage treatment showed minimal effects on both. Intraperitoneal and topical treatment decreased infiltration and hyperplasia with an increase in the level of hyperkeratosis. Conclusion: These findings demonstrate that DCOE has remarkable antitumor activity against DMBA-induced skin cancer compared with non-treated animals paving the ground for further investigations. |
| eu_rights_str_mv | openAccess |
| format | article |
| id | LAURepo_794485e9971d01394e14e01864b4c8fd |
| identifier_str_mv | 1388-0209 Zeinab, R. A., Mroueh, M., Diab-Assaf, M., Jurjus, A., Wex, B., Sakr, A., & Daher, C. F. (2011). Chemopreventive effects of wild carrot oil against 7, 12-dimethyl benz (a) anthracene-induced squamous cell carcinoma in mice. Pharmaceutical biology, 49(9), 955-961. |
| language_invalid_str_mv | en |
| network_acronym_str | LAURepo |
| network_name_str | Lebanese American University repository |
| oai_identifier_str | oai:laur.lau.edu.lb:10725/2745 |
| publishDate | 2011 |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| spelling | Chemopreventive effects of wild carrot oil against 7,12-dimethyl benz(a)anthracene-induced squamous cell carcinoma in miceAbu Zeinab, RamiMroueh, MohamadDiab-Assaf, MonaJurjus, AbdoWex, BrigitteSakr, AmerDaher, Costantine F.Context: Daucus carota L. ssp. carota (Apiacea) is widely distributed throughout the world and has many uses in traditional medicine. Objective: The present study investigates the chemopreventive effects of oil extract of D. carota umbels on 7,12-dimethyl benz(a)anthracene (DMBA)-induced skin cancer in mice. Materials and methods: D. carota oil extract (DCOE) was prepared by extracting the dried umbels with 50:50 acetone:methanol. Skin papilloma were initiated by DMBA and promoted by 12-O-tetradecanoyl phorobol-13-acetate (TPA). The extract was administered to animals via gavage (0.02 mL of 100% oil), intraperitoneal (0.3 mL of 2% oil), and topical (0.2 mL of 5, 50, and 100% oil) routes for 20 weeks. Tumor appearance, incidence, yield, and volume were compared with those of a non-treated control group. Results: Topical 100% treatment delayed tumor appearance, and inhibited tumor incidence and yield by 40 and 89%, respectively. Topical 50% treatment inhibited tumor incidence and yield by 30 and 83%, respectively, whereas the 5% treatment inhibited tumor yield by 36%. Tumor volume was decreased by 99, 91, and 70% following topical treatments with 100, 50, and 5% oil, respectively. Intraperitoneal treatment inhibited tumor yield by 43%, and decreased tumor volume by 85%, whereas gavage treatment showed minimal effects on both. Intraperitoneal and topical treatment decreased infiltration and hyperplasia with an increase in the level of hyperkeratosis. Conclusion: These findings demonstrate that DCOE has remarkable antitumor activity against DMBA-induced skin cancer compared with non-treated animals paving the ground for further investigations.PublishedN/A2015-12-01T08:58:50Z2015-12-01T08:58:50Z2011-07-212015-12-01Articleinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1388-0209http://hdl.handle.net/10725/2745http://dx.doi.org/10.3109/13880209.2011.559250Zeinab, R. A., Mroueh, M., Diab-Assaf, M., Jurjus, A., Wex, B., Sakr, A., & Daher, C. F. (2011). Chemopreventive effects of wild carrot oil against 7, 12-dimethyl benz (a) anthracene-induced squamous cell carcinoma in mice. Pharmaceutical biology, 49(9), 955-961.http://www.tandfonline.com/doi/abs/10.3109/13880209.2011.559250enPharmaceutical Biologyinfo:eu-repo/semantics/openAccessoai:laur.lau.edu.lb:10725/27452024-01-31T14:44:33Z |
| spellingShingle | Chemopreventive effects of wild carrot oil against 7,12-dimethyl benz(a)anthracene-induced squamous cell carcinoma in mice Abu Zeinab, Rami |
| status_str | publishedVersion |
| title | Chemopreventive effects of wild carrot oil against 7,12-dimethyl benz(a)anthracene-induced squamous cell carcinoma in mice |
| title_full | Chemopreventive effects of wild carrot oil against 7,12-dimethyl benz(a)anthracene-induced squamous cell carcinoma in mice |
| title_fullStr | Chemopreventive effects of wild carrot oil against 7,12-dimethyl benz(a)anthracene-induced squamous cell carcinoma in mice |
| title_full_unstemmed | Chemopreventive effects of wild carrot oil against 7,12-dimethyl benz(a)anthracene-induced squamous cell carcinoma in mice |
| title_short | Chemopreventive effects of wild carrot oil against 7,12-dimethyl benz(a)anthracene-induced squamous cell carcinoma in mice |
| title_sort | Chemopreventive effects of wild carrot oil against 7,12-dimethyl benz(a)anthracene-induced squamous cell carcinoma in mice |
| url | http://hdl.handle.net/10725/2745 http://dx.doi.org/10.3109/13880209.2011.559250 http://www.tandfonline.com/doi/abs/10.3109/13880209.2011.559250 |