Nonsuperoxide lucigenin-enhanced chemiluminescence from phospholipids and human saphenous veins
The use of lucigenin-enhanced chemiluminescence (CL) for the detection of superoxide (O2·−) has grown in popularity due to an increased demand for a simple and specific system capable of measuring superoxide. In this study we report a lucigenin-CL signal emanating from human saphenous veins (SV) tha...
Saved in:
| Main Author: | |
|---|---|
| Other Authors: | , , |
| Format: | article |
| Published: |
1995
|
| Online Access: | http://hdl.handle.net/10725/6340 https://doi.org/10.1016/0891-5849(94)E0140-E http://libraries.lau.edu.lb/research/laur/terms-of-use/articles.php http://www.sciencedirect.com/science/article/pii/0891584994E0140E |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1864513479008845824 |
|---|---|
| author | Capomacchia, Anthony C. |
| author2 | Barber, Dustan A. Do, Ngoc H. Tackett, Randall L. |
| author2_role | author author author |
| author_facet | Capomacchia, Anthony C. Barber, Dustan A. Do, Ngoc H. Tackett, Randall L. |
| author_role | author |
| dc.creator.none.fl_str_mv | Capomacchia, Anthony C. Barber, Dustan A. Do, Ngoc H. Tackett, Randall L. |
| dc.date.none.fl_str_mv | 1995 2017-10-18T06:27:03Z 2017-10-18T06:27:03Z 2017-10-18 |
| dc.identifier.none.fl_str_mv | 1873-4596 http://hdl.handle.net/10725/6340 https://doi.org/10.1016/0891-5849(94)E0140-E Barber, D. A., Do, N. H., Tackett, R. L., & Capomacchia, A. C. (1995). Nonsuperoxide lucigenin-enhanced chemiluminescence from phospholipids and human saphenous veins. Free Radical Biology and Medicine, 18(3), 565-569. http://libraries.lau.edu.lb/research/laur/terms-of-use/articles.php http://www.sciencedirect.com/science/article/pii/0891584994E0140E |
| dc.language.none.fl_str_mv | en |
| dc.relation.none.fl_str_mv | Free Radical Biology and Medicine |
| dc.rights.*.fl_str_mv | info:eu-repo/semantics/openAccess |
| dc.title.none.fl_str_mv | Nonsuperoxide lucigenin-enhanced chemiluminescence from phospholipids and human saphenous veins |
| dc.type.none.fl_str_mv | Article info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article |
| description | The use of lucigenin-enhanced chemiluminescence (CL) for the detection of superoxide (O2·−) has grown in popularity due to an increased demand for a simple and specific system capable of measuring superoxide. In this study we report a lucigenin-CL signal emanating from human saphenous veins (SV) that was not inhibited by superoxide dismutase (SOD) and lasted for more than 24 h. A larger CL-signal with similar properties was produced by saphenous veins that had been dehydrated. A similar, non-SOD-inhibitable lucigenin-CL was also produced with a variety of phospholipids and phosphatidic acid. The chemical moiety responsible for the phospholipid CL is oxygen dependent but remains unidentified because a variety of lipids and phosphate containing species failed to produce such a signal. These results suggest that the use of lucigenin as a specific CL enhancer for O2·− must be clearly discriminated with a specific O2·− inhibitor when used in biological systems. |
| eu_rights_str_mv | openAccess |
| format | article |
| id | LAURepo_79f97f3465d7bcd29754362d76a7f87d |
| identifier_str_mv | 1873-4596 Barber, D. A., Do, N. H., Tackett, R. L., & Capomacchia, A. C. (1995). Nonsuperoxide lucigenin-enhanced chemiluminescence from phospholipids and human saphenous veins. Free Radical Biology and Medicine, 18(3), 565-569. |
| language_invalid_str_mv | en |
| network_acronym_str | LAURepo |
| network_name_str | Lebanese American University repository |
| oai_identifier_str | oai:laur.lau.edu.lb:10725/6340 |
| publishDate | 1995 |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| spelling | Nonsuperoxide lucigenin-enhanced chemiluminescence from phospholipids and human saphenous veinsCapomacchia, Anthony C.Barber, Dustan A.Do, Ngoc H.Tackett, Randall L.The use of lucigenin-enhanced chemiluminescence (CL) for the detection of superoxide (O2·−) has grown in popularity due to an increased demand for a simple and specific system capable of measuring superoxide. In this study we report a lucigenin-CL signal emanating from human saphenous veins (SV) that was not inhibited by superoxide dismutase (SOD) and lasted for more than 24 h. A larger CL-signal with similar properties was produced by saphenous veins that had been dehydrated. A similar, non-SOD-inhibitable lucigenin-CL was also produced with a variety of phospholipids and phosphatidic acid. The chemical moiety responsible for the phospholipid CL is oxygen dependent but remains unidentified because a variety of lipids and phosphate containing species failed to produce such a signal. These results suggest that the use of lucigenin as a specific CL enhancer for O2·− must be clearly discriminated with a specific O2·− inhibitor when used in biological systems.PublishedN/A2017-10-18T06:27:03Z2017-10-18T06:27:03Z19952017-10-18Articleinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1873-4596http://hdl.handle.net/10725/6340https://doi.org/10.1016/0891-5849(94)E0140-EBarber, D. A., Do, N. H., Tackett, R. L., & Capomacchia, A. C. (1995). Nonsuperoxide lucigenin-enhanced chemiluminescence from phospholipids and human saphenous veins. Free Radical Biology and Medicine, 18(3), 565-569.http://libraries.lau.edu.lb/research/laur/terms-of-use/articles.phphttp://www.sciencedirect.com/science/article/pii/0891584994E0140EenFree Radical Biology and Medicineinfo:eu-repo/semantics/openAccessoai:laur.lau.edu.lb:10725/63402021-03-19T10:43:10Z |
| spellingShingle | Nonsuperoxide lucigenin-enhanced chemiluminescence from phospholipids and human saphenous veins Capomacchia, Anthony C. |
| status_str | publishedVersion |
| title | Nonsuperoxide lucigenin-enhanced chemiluminescence from phospholipids and human saphenous veins |
| title_full | Nonsuperoxide lucigenin-enhanced chemiluminescence from phospholipids and human saphenous veins |
| title_fullStr | Nonsuperoxide lucigenin-enhanced chemiluminescence from phospholipids and human saphenous veins |
| title_full_unstemmed | Nonsuperoxide lucigenin-enhanced chemiluminescence from phospholipids and human saphenous veins |
| title_short | Nonsuperoxide lucigenin-enhanced chemiluminescence from phospholipids and human saphenous veins |
| title_sort | Nonsuperoxide lucigenin-enhanced chemiluminescence from phospholipids and human saphenous veins |
| url | http://hdl.handle.net/10725/6340 https://doi.org/10.1016/0891-5849(94)E0140-E http://libraries.lau.edu.lb/research/laur/terms-of-use/articles.php http://www.sciencedirect.com/science/article/pii/0891584994E0140E |