The value of amniotic fluid interleukin-6 determination in patients with preterm labor and intact membranes in the detection of microbial invasion of the amniotic cavity
Objectives: Our purpose was to (1) determine the value of amniotic fluid interleukin-6 determination in the detection of microbial invasion of the amniotic cavity and (2) compare interleukin-6 to other rapid tests in the evaluation of preterm labor. Study Design: Amniotic fluid interleukin-6 was det...
محفوظ في:
| المؤلف الرئيسي: | |
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| مؤلفون آخرون: | , , , , |
| التنسيق: | article |
| منشور في: |
1994
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| الوصول للمادة أونلاين: | http://hdl.handle.net/10725/10461 https://doi.org/10.1016/S0002-9378(94)70057-5 http://libraries.lau.edu.lb/research/laur/terms-of-use/articles.php https://www.sciencedirect.com/science/article/pii/S0002937894700575 |
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| _version_ | 1864513476634869760 |
|---|---|
| author | Khoury, Alfred |
| author2 | Coultrip, Laura L. Lien, Jean Marie Gomez, Ricardo Kapernick, Peter Ghossman, J.H. |
| author2_role | author author author author author |
| author_facet | Khoury, Alfred Coultrip, Laura L. Lien, Jean Marie Gomez, Ricardo Kapernick, Peter Ghossman, J.H. |
| author_role | author |
| dc.creator.none.fl_str_mv | Khoury, Alfred Coultrip, Laura L. Lien, Jean Marie Gomez, Ricardo Kapernick, Peter Ghossman, J.H. |
| dc.date.none.fl_str_mv | 1994 2019-04-16T12:38:39Z 2019-04-16T12:38:39Z 2019-04-16 |
| dc.identifier.none.fl_str_mv | 1097-6868 http://hdl.handle.net/10725/10461 https://doi.org/10.1016/S0002-9378(94)70057-5 Coultrip, L. L., Lien, J. M., Gomez, R., Kapernick, P., Khoury, A., & Grossman, J. H. (1994). The value of amniotic fluid interleukin-6 determination in patients with preterm labor and intact membranes in the detection of microbial invasion of the amniotic cavity. American journal of obstetrics and gynecology, 171(4), 901-911. http://libraries.lau.edu.lb/research/laur/terms-of-use/articles.php https://www.sciencedirect.com/science/article/pii/S0002937894700575 |
| dc.language.none.fl_str_mv | en |
| dc.relation.none.fl_str_mv | American Journal of Obstetrics and Gynecology |
| dc.rights.*.fl_str_mv | info:eu-repo/semantics/openAccess |
| dc.title.none.fl_str_mv | The value of amniotic fluid interleukin-6 determination in patients with preterm labor and intact membranes in the detection of microbial invasion of the amniotic cavity |
| dc.type.none.fl_str_mv | Article info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article |
| description | Objectives: Our purpose was to (1) determine the value of amniotic fluid interleukin-6 determination in the detection of microbial invasion of the amniotic cavity and (2) compare interleukin-6 to other rapid tests in the evaluation of preterm labor. Study Design: Amniotic fluid interleukin-6 was determined quantitatively by enzyme-linked immunosorbent assay in 91 amniotic fluid specimens obtained by amniocentesis in 89 patients with preterm labor. Amniotic fluid cultures for aerobes, anaerobes, and mycoplasma species were performed. Receiver-operator characteristic curve analysis, logistic regression analysis, and Cox's proportional-hazards model were used to explore the relationship between several explanatory and outcome variables. Diagnostic index values of interleukin-6, glucose level, Gram stain, leukocyte esterase; and limulus amebocyte lysate assay for prediction of a positive amniotic fluid culture, preterm delivery, clinical infection, and neonatal sepsis were calculated. Results: The prevalence of positive amniotic fluid cultures was 13% (12/89). The median interleukin-6 concentration in patients with positive cultures was 241.8 ng/ml, in contrast to 0.291 ng/ml in patients with negative cultures (p. < 0.005). Sensitivity and specificity of an interleukin-6 level ≥ 6.17 ng/ml was 75% and 79%, in contrast to that of glucose, ≤ 12 mg/dl (83% and 86%) for a positive amniotic fluid culture and sensitivity (p = 0.26, z test). All patients with an interleukin-6 level>6.17 ng/ml were delivered preterm, in contrast to 85.2% of patients with a glucose level ≤ 12 mg/dl. When all rapid tests and clinical parameters were considered simultaneously in the logistic model, only interleukin-6 maintained a significant relationship to preterm birth (odds ratio 35, p = 0.003). Cox's proportional analysis demonstrated a strong relationship between interleukin-6 and the amniocentesis-to-delivery interval after clinical variables were controlled for (hazard ratio 3.01, p < 0.00001). Conclusion: Amniotic fluid interleukin-6 determination may be a useful adjunct to our armamentarium of rapid tests to exclude infection and predict delivery in patients with preterm labor and intact membranes. |
| eu_rights_str_mv | openAccess |
| format | article |
| id | LAURepo_7c26c05bc7a057f8998d2f50571f6b5d |
| identifier_str_mv | 1097-6868 Coultrip, L. L., Lien, J. M., Gomez, R., Kapernick, P., Khoury, A., & Grossman, J. H. (1994). The value of amniotic fluid interleukin-6 determination in patients with preterm labor and intact membranes in the detection of microbial invasion of the amniotic cavity. American journal of obstetrics and gynecology, 171(4), 901-911. |
| language_invalid_str_mv | en |
| network_acronym_str | LAURepo |
| network_name_str | Lebanese American University repository |
| oai_identifier_str | oai:laur.lau.edu.lb:10725/10461 |
| publishDate | 1994 |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| spelling | The value of amniotic fluid interleukin-6 determination in patients with preterm labor and intact membranes in the detection of microbial invasion of the amniotic cavityKhoury, AlfredCoultrip, Laura L.Lien, Jean MarieGomez, RicardoKapernick, PeterGhossman, J.H.Objectives: Our purpose was to (1) determine the value of amniotic fluid interleukin-6 determination in the detection of microbial invasion of the amniotic cavity and (2) compare interleukin-6 to other rapid tests in the evaluation of preterm labor. Study Design: Amniotic fluid interleukin-6 was determined quantitatively by enzyme-linked immunosorbent assay in 91 amniotic fluid specimens obtained by amniocentesis in 89 patients with preterm labor. Amniotic fluid cultures for aerobes, anaerobes, and mycoplasma species were performed. Receiver-operator characteristic curve analysis, logistic regression analysis, and Cox's proportional-hazards model were used to explore the relationship between several explanatory and outcome variables. Diagnostic index values of interleukin-6, glucose level, Gram stain, leukocyte esterase; and limulus amebocyte lysate assay for prediction of a positive amniotic fluid culture, preterm delivery, clinical infection, and neonatal sepsis were calculated. Results: The prevalence of positive amniotic fluid cultures was 13% (12/89). The median interleukin-6 concentration in patients with positive cultures was 241.8 ng/ml, in contrast to 0.291 ng/ml in patients with negative cultures (p. < 0.005). Sensitivity and specificity of an interleukin-6 level ≥ 6.17 ng/ml was 75% and 79%, in contrast to that of glucose, ≤ 12 mg/dl (83% and 86%) for a positive amniotic fluid culture and sensitivity (p = 0.26, z test). All patients with an interleukin-6 level>6.17 ng/ml were delivered preterm, in contrast to 85.2% of patients with a glucose level ≤ 12 mg/dl. When all rapid tests and clinical parameters were considered simultaneously in the logistic model, only interleukin-6 maintained a significant relationship to preterm birth (odds ratio 35, p = 0.003). Cox's proportional analysis demonstrated a strong relationship between interleukin-6 and the amniocentesis-to-delivery interval after clinical variables were controlled for (hazard ratio 3.01, p < 0.00001). Conclusion: Amniotic fluid interleukin-6 determination may be a useful adjunct to our armamentarium of rapid tests to exclude infection and predict delivery in patients with preterm labor and intact membranes.PublishedN/A2019-04-16T12:38:39Z2019-04-16T12:38:39Z19942019-04-16Articleinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1097-6868http://hdl.handle.net/10725/10461https://doi.org/10.1016/S0002-9378(94)70057-5Coultrip, L. L., Lien, J. M., Gomez, R., Kapernick, P., Khoury, A., & Grossman, J. H. (1994). The value of amniotic fluid interleukin-6 determination in patients with preterm labor and intact membranes in the detection of microbial invasion of the amniotic cavity. American journal of obstetrics and gynecology, 171(4), 901-911.http://libraries.lau.edu.lb/research/laur/terms-of-use/articles.phphttps://www.sciencedirect.com/science/article/pii/S0002937894700575enAmerican Journal of Obstetrics and Gynecologyinfo:eu-repo/semantics/openAccessoai:laur.lau.edu.lb:10725/104612021-03-19T10:45:30Z |
| spellingShingle | The value of amniotic fluid interleukin-6 determination in patients with preterm labor and intact membranes in the detection of microbial invasion of the amniotic cavity Khoury, Alfred |
| status_str | publishedVersion |
| title | The value of amniotic fluid interleukin-6 determination in patients with preterm labor and intact membranes in the detection of microbial invasion of the amniotic cavity |
| title_full | The value of amniotic fluid interleukin-6 determination in patients with preterm labor and intact membranes in the detection of microbial invasion of the amniotic cavity |
| title_fullStr | The value of amniotic fluid interleukin-6 determination in patients with preterm labor and intact membranes in the detection of microbial invasion of the amniotic cavity |
| title_full_unstemmed | The value of amniotic fluid interleukin-6 determination in patients with preterm labor and intact membranes in the detection of microbial invasion of the amniotic cavity |
| title_short | The value of amniotic fluid interleukin-6 determination in patients with preterm labor and intact membranes in the detection of microbial invasion of the amniotic cavity |
| title_sort | The value of amniotic fluid interleukin-6 determination in patients with preterm labor and intact membranes in the detection of microbial invasion of the amniotic cavity |
| url | http://hdl.handle.net/10725/10461 https://doi.org/10.1016/S0002-9378(94)70057-5 http://libraries.lau.edu.lb/research/laur/terms-of-use/articles.php https://www.sciencedirect.com/science/article/pii/S0002937894700575 |