Identification of Sphingomonads on the Basis of Polymerase Chain Reaction Amplified 16S rRNA Gene
Sphingomonas is a genus that is basically of environmental origin but can also be associated with health hazards, especially in the hospital environment where there is a great need to properly monitor water sources. The abundance and frequent isolation of derivatives of yellow pigmented colonies fro...
محفوظ في:
| المؤلف الرئيسي: | |
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| مؤلفون آخرون: | , |
| التنسيق: | article |
| منشور في: |
2008
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| الوصول للمادة أونلاين: | http://hdl.handle.net/10725/3402 http://web.a.ebscohost.com/abstract?site=ehost&scope=site&jrnl=12013080&AN=43199598&h=fgiiX2s%2bscW4uzo2Xws4Y3eAEO7za5VjX71ZV67hei0D2%2bWFeZ5eSP8SA1POaQnm2wVrletzsAiTEpJrppzDMA%3d%3d&crl=c&resultLocal=ErrCrlNoResults&resultNs=Ehost&crlhashurl=login.aspx%3fdirect%3dtrue%26profile%3dehost%26scope%3dsite%26authtype%3dcrawler%26jrnl%3d12013080%26AN%3d43199598 |
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| الملخص: | Sphingomonas is a genus that is basically of environmental origin but can also be associated with health hazards, especially in the hospital environment where there is a great need to properly monitor water sources. The abundance and frequent isolation of derivatives of yellow pigmented colonies from drinking water samples in Lebanon — where an intermittent mode of supply is employed, and which induces frequent biofilm sloughing — necessitated the establishment of a rapid and feasible assay to screen specifically for sphingomonads. In this study, 50 isolates recovered from drinking water with yellow- to orange-pigmented colonies were used to establish a polymerase chain reaction-based (PCR-based) screening assay. The use of sphingomonad specific modified primers gave one common band with a size of 320 bp in all presumptive and sequence confirmed sphingomonads. However, no amplification was observed with Escherichia coli, Staphylococcus aureus, and Pseudomonas aeruginosa. Applying the PCR-based assay described in this paper increased both the efficiency and the reliability of screening for sphingomonads in water samples, thereby minimizing related risk factors. |
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