Identification of Sphingomonads on the Basis of Polymerase Chain Reaction Amplified 16S rRNA Gene
Sphingomonas is a genus that is basically of environmental origin but can also be associated with health hazards, especially in the hospital environment where there is a great need to properly monitor water sources. The abundance and frequent isolation of derivatives of yellow pigmented colonies fro...
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2008
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| Online Access: | http://hdl.handle.net/10725/3402 http://web.a.ebscohost.com/abstract?site=ehost&scope=site&jrnl=12013080&AN=43199598&h=fgiiX2s%2bscW4uzo2Xws4Y3eAEO7za5VjX71ZV67hei0D2%2bWFeZ5eSP8SA1POaQnm2wVrletzsAiTEpJrppzDMA%3d%3d&crl=c&resultLocal=ErrCrlNoResults&resultNs=Ehost&crlhashurl=login.aspx%3fdirect%3dtrue%26profile%3dehost%26scope%3dsite%26authtype%3dcrawler%26jrnl%3d12013080%26AN%3d43199598 |
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| _version_ | 1864513474014478336 |
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| author | Tokajian, Sima |
| author2 | Farah, Maya Hashwa, Fuad |
| author2_role | author author |
| author_facet | Tokajian, Sima Farah, Maya Hashwa, Fuad |
| author_role | author |
| dc.creator.none.fl_str_mv | Tokajian, Sima Farah, Maya Hashwa, Fuad |
| dc.date.none.fl_str_mv | 2008 2016-03-23T12:38:03Z 2016-03-23T12:38:03Z 2016-03-23 |
| dc.identifier.none.fl_str_mv | 1201-3080 http://hdl.handle.net/10725/3402 Tokajian, S., Farah, M., & Hashwa, F. (2008). Identification of Sphingomonads on the Basis of Polymerase Chain Reaction Amplified 16S rRNA Gene. Water Quality Research Journal of Canada (Canadian Association on Water Quality), 43(4). http://web.a.ebscohost.com/abstract?site=ehost&scope=site&jrnl=12013080&AN=43199598&h=fgiiX2s%2bscW4uzo2Xws4Y3eAEO7za5VjX71ZV67hei0D2%2bWFeZ5eSP8SA1POaQnm2wVrletzsAiTEpJrppzDMA%3d%3d&crl=c&resultLocal=ErrCrlNoResults&resultNs=Ehost&crlhashurl=login.aspx%3fdirect%3dtrue%26profile%3dehost%26scope%3dsite%26authtype%3dcrawler%26jrnl%3d12013080%26AN%3d43199598 |
| dc.language.none.fl_str_mv | en |
| dc.relation.none.fl_str_mv | Water Quality Research Journal of Canada |
| dc.rights.*.fl_str_mv | info:eu-repo/semantics/openAccess |
| dc.title.none.fl_str_mv | Identification of Sphingomonads on the Basis of Polymerase Chain Reaction Amplified 16S rRNA Gene |
| dc.type.none.fl_str_mv | Article info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article |
| description | Sphingomonas is a genus that is basically of environmental origin but can also be associated with health hazards, especially in the hospital environment where there is a great need to properly monitor water sources. The abundance and frequent isolation of derivatives of yellow pigmented colonies from drinking water samples in Lebanon — where an intermittent mode of supply is employed, and which induces frequent biofilm sloughing — necessitated the establishment of a rapid and feasible assay to screen specifically for sphingomonads. In this study, 50 isolates recovered from drinking water with yellow- to orange-pigmented colonies were used to establish a polymerase chain reaction-based (PCR-based) screening assay. The use of sphingomonad specific modified primers gave one common band with a size of 320 bp in all presumptive and sequence confirmed sphingomonads. However, no amplification was observed with Escherichia coli, Staphylococcus aureus, and Pseudomonas aeruginosa. Applying the PCR-based assay described in this paper increased both the efficiency and the reliability of screening for sphingomonads in water samples, thereby minimizing related risk factors. |
| eu_rights_str_mv | openAccess |
| format | article |
| id | LAURepo_81b2f4eb099c09d4db58b1a32c42b1cf |
| identifier_str_mv | 1201-3080 Tokajian, S., Farah, M., & Hashwa, F. (2008). Identification of Sphingomonads on the Basis of Polymerase Chain Reaction Amplified 16S rRNA Gene. Water Quality Research Journal of Canada (Canadian Association on Water Quality), 43(4). |
| language_invalid_str_mv | en |
| network_acronym_str | LAURepo |
| network_name_str | Lebanese American University repository |
| oai_identifier_str | oai:laur.lau.edu.lb:10725/3402 |
| publishDate | 2008 |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| spelling | Identification of Sphingomonads on the Basis of Polymerase Chain Reaction Amplified 16S rRNA GeneTokajian, SimaFarah, MayaHashwa, FuadSphingomonas is a genus that is basically of environmental origin but can also be associated with health hazards, especially in the hospital environment where there is a great need to properly monitor water sources. The abundance and frequent isolation of derivatives of yellow pigmented colonies from drinking water samples in Lebanon — where an intermittent mode of supply is employed, and which induces frequent biofilm sloughing — necessitated the establishment of a rapid and feasible assay to screen specifically for sphingomonads. In this study, 50 isolates recovered from drinking water with yellow- to orange-pigmented colonies were used to establish a polymerase chain reaction-based (PCR-based) screening assay. The use of sphingomonad specific modified primers gave one common band with a size of 320 bp in all presumptive and sequence confirmed sphingomonads. However, no amplification was observed with Escherichia coli, Staphylococcus aureus, and Pseudomonas aeruginosa. Applying the PCR-based assay described in this paper increased both the efficiency and the reliability of screening for sphingomonads in water samples, thereby minimizing related risk factors.PublishedN/A2016-03-23T12:38:03Z2016-03-23T12:38:03Z20082016-03-23Articleinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1201-3080http://hdl.handle.net/10725/3402Tokajian, S., Farah, M., & Hashwa, F. (2008). Identification of Sphingomonads on the Basis of Polymerase Chain Reaction Amplified 16S rRNA Gene. Water Quality Research Journal of Canada (Canadian Association on Water Quality), 43(4).http://web.a.ebscohost.com/abstract?site=ehost&scope=site&jrnl=12013080&AN=43199598&h=fgiiX2s%2bscW4uzo2Xws4Y3eAEO7za5VjX71ZV67hei0D2%2bWFeZ5eSP8SA1POaQnm2wVrletzsAiTEpJrppzDMA%3d%3d&crl=c&resultLocal=ErrCrlNoResults&resultNs=Ehost&crlhashurl=login.aspx%3fdirect%3dtrue%26profile%3dehost%26scope%3dsite%26authtype%3dcrawler%26jrnl%3d12013080%26AN%3d43199598enWater Quality Research Journal of Canadainfo:eu-repo/semantics/openAccessoai:laur.lau.edu.lb:10725/34022019-02-19T14:02:26Z |
| spellingShingle | Identification of Sphingomonads on the Basis of Polymerase Chain Reaction Amplified 16S rRNA Gene Tokajian, Sima |
| status_str | publishedVersion |
| title | Identification of Sphingomonads on the Basis of Polymerase Chain Reaction Amplified 16S rRNA Gene |
| title_full | Identification of Sphingomonads on the Basis of Polymerase Chain Reaction Amplified 16S rRNA Gene |
| title_fullStr | Identification of Sphingomonads on the Basis of Polymerase Chain Reaction Amplified 16S rRNA Gene |
| title_full_unstemmed | Identification of Sphingomonads on the Basis of Polymerase Chain Reaction Amplified 16S rRNA Gene |
| title_short | Identification of Sphingomonads on the Basis of Polymerase Chain Reaction Amplified 16S rRNA Gene |
| title_sort | Identification of Sphingomonads on the Basis of Polymerase Chain Reaction Amplified 16S rRNA Gene |
| url | http://hdl.handle.net/10725/3402 http://web.a.ebscohost.com/abstract?site=ehost&scope=site&jrnl=12013080&AN=43199598&h=fgiiX2s%2bscW4uzo2Xws4Y3eAEO7za5VjX71ZV67hei0D2%2bWFeZ5eSP8SA1POaQnm2wVrletzsAiTEpJrppzDMA%3d%3d&crl=c&resultLocal=ErrCrlNoResults&resultNs=Ehost&crlhashurl=login.aspx%3fdirect%3dtrue%26profile%3dehost%26scope%3dsite%26authtype%3dcrawler%26jrnl%3d12013080%26AN%3d43199598 |