The Antioxidant and Pro-apoptotic effects of Sternbergia clusiana Bulb Ethanolic Extract on Breast Cancer Cells in vitro
Extensive research has revealed that Amaryllidaceae species exhibit numerous therapeutic properties through several mechanisms. Among the various Amaryllidaceae species, Sternbergia clusiana has been recently given a great amount of attention in the medicinal field due to its richness in small chemi...
محفوظ في:
| المؤلف الرئيسي: | |
|---|---|
| التنسيق: | masterThesis |
| منشور في: |
2021
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| الموضوعات: | |
| الوصول للمادة أونلاين: | http://hdl.handle.net/10725/13689 https://doi.org/10.26756/th.2022.225 http://libraries.lau.edu.lb/research/laur/terms-of-use/thesis.php |
| الوسوم: |
إضافة وسم
لا توجد وسوم, كن أول من يضع وسما على هذه التسجيلة!
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| _version_ | 1864513467855142912 |
|---|---|
| author | El Samarji, Mona |
| author_facet | El Samarji, Mona |
| author_role | author |
| dc.creator.none.fl_str_mv | El Samarji, Mona |
| dc.date.none.fl_str_mv | 2021 2021-04-29 2022-06-16T05:50:14Z 2022-06-16T05:50:14Z |
| dc.identifier.none.fl_str_mv | http://hdl.handle.net/10725/13689 https://doi.org/10.26756/th.2022.225 http://libraries.lau.edu.lb/research/laur/terms-of-use/thesis.php |
| dc.language.none.fl_str_mv | en |
| dc.publisher.none.fl_str_mv | Lebanese American University |
| dc.rights.*.fl_str_mv | info:eu-repo/semantics/openAccess |
| dc.subject.none.fl_str_mv | Breast -- Cancer -- Molecular aspects Breast -- Cancer -- Treatment Apoptosis Cancer cells -- Proliferation Lebanese American University -- Dissertations Dissertations, Academic |
| dc.title.none.fl_str_mv | The Antioxidant and Pro-apoptotic effects of Sternbergia clusiana Bulb Ethanolic Extract on Breast Cancer Cells in vitro |
| dc.type.none.fl_str_mv | Thesis info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/masterThesis |
| description | Extensive research has revealed that Amaryllidaceae species exhibit numerous therapeutic properties through several mechanisms. Among the various Amaryllidaceae species, Sternbergia clusiana has been recently given a great amount of attention in the medicinal field due to its richness in small chemical molecules, namely Amaryllidaceae alkaloids; these have been reported to possess an antioxidant and anti-inflammatory role, in addition to a prominent anti-cancer effect on different types of cancer cell lines. The current study investigates the selective anti-proliferative effect of Sternbergia clusiana bulb ethanolic extract (SbBEE) on breast cancer cells cultured in vitro (MDA-MB-231 and MCF-7) and reveals the underlying mechanism responsible for the induction of cell death. MDA-MB-231 cells treated with SbBEE exhibited morphological and biochemical hallmarks of apoptosis that were detected through DNA fragmentation, the hyper-expression of phosphatidylserine on the outer leaflet of cells membrane and the upregulation of proteins able to mediate cell death through apoptosis (c-PARP, Bax/ Bcl2). The prominent decrease in ROS levels upon treatment confirmed the antioxidant property of the extract being investigated, highlighting the promising effect of SbBEE in breast cancer treatment. |
| eu_rights_str_mv | openAccess |
| format | masterThesis |
| id | LAURepo_83cbe9b1fd24fe9cfdb1c70101c0c286 |
| language_invalid_str_mv | en |
| network_acronym_str | LAURepo |
| network_name_str | Lebanese American University repository |
| oai_identifier_str | oai:laur.lau.edu.lb:10725/13689 |
| publishDate | 2021 |
| publisher.none.fl_str_mv | Lebanese American University |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| spelling | The Antioxidant and Pro-apoptotic effects of Sternbergia clusiana Bulb Ethanolic Extract on Breast Cancer Cells in vitroEl Samarji, MonaBreast -- Cancer -- Molecular aspectsBreast -- Cancer -- TreatmentApoptosisCancer cells -- ProliferationLebanese American University -- DissertationsDissertations, AcademicExtensive research has revealed that Amaryllidaceae species exhibit numerous therapeutic properties through several mechanisms. Among the various Amaryllidaceae species, Sternbergia clusiana has been recently given a great amount of attention in the medicinal field due to its richness in small chemical molecules, namely Amaryllidaceae alkaloids; these have been reported to possess an antioxidant and anti-inflammatory role, in addition to a prominent anti-cancer effect on different types of cancer cell lines. The current study investigates the selective anti-proliferative effect of Sternbergia clusiana bulb ethanolic extract (SbBEE) on breast cancer cells cultured in vitro (MDA-MB-231 and MCF-7) and reveals the underlying mechanism responsible for the induction of cell death. MDA-MB-231 cells treated with SbBEE exhibited morphological and biochemical hallmarks of apoptosis that were detected through DNA fragmentation, the hyper-expression of phosphatidylserine on the outer leaflet of cells membrane and the upregulation of proteins able to mediate cell death through apoptosis (c-PARP, Bax/ Bcl2). The prominent decrease in ROS levels upon treatment confirmed the antioxidant property of the extract being investigated, highlighting the promising effect of SbBEE in breast cancer treatment.1 online resource (xi, 57 leaves): ill. (some col.)Bibliography: leaf 50-57Lebanese American University2022-06-16T05:50:14Z2022-06-16T05:50:14Z20212021-04-29Thesisinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesishttp://hdl.handle.net/10725/13689https://doi.org/10.26756/th.2022.225http://libraries.lau.edu.lb/research/laur/terms-of-use/thesis.phpeninfo:eu-repo/semantics/openAccessoai:laur.lau.edu.lb:10725/136892023-02-20T12:08:53Z |
| spellingShingle | The Antioxidant and Pro-apoptotic effects of Sternbergia clusiana Bulb Ethanolic Extract on Breast Cancer Cells in vitro El Samarji, Mona Breast -- Cancer -- Molecular aspects Breast -- Cancer -- Treatment Apoptosis Cancer cells -- Proliferation Lebanese American University -- Dissertations Dissertations, Academic |
| status_str | publishedVersion |
| title | The Antioxidant and Pro-apoptotic effects of Sternbergia clusiana Bulb Ethanolic Extract on Breast Cancer Cells in vitro |
| title_full | The Antioxidant and Pro-apoptotic effects of Sternbergia clusiana Bulb Ethanolic Extract on Breast Cancer Cells in vitro |
| title_fullStr | The Antioxidant and Pro-apoptotic effects of Sternbergia clusiana Bulb Ethanolic Extract on Breast Cancer Cells in vitro |
| title_full_unstemmed | The Antioxidant and Pro-apoptotic effects of Sternbergia clusiana Bulb Ethanolic Extract on Breast Cancer Cells in vitro |
| title_short | The Antioxidant and Pro-apoptotic effects of Sternbergia clusiana Bulb Ethanolic Extract on Breast Cancer Cells in vitro |
| title_sort | The Antioxidant and Pro-apoptotic effects of Sternbergia clusiana Bulb Ethanolic Extract on Breast Cancer Cells in vitro |
| topic | Breast -- Cancer -- Molecular aspects Breast -- Cancer -- Treatment Apoptosis Cancer cells -- Proliferation Lebanese American University -- Dissertations Dissertations, Academic |
| url | http://hdl.handle.net/10725/13689 https://doi.org/10.26756/th.2022.225 http://libraries.lau.edu.lb/research/laur/terms-of-use/thesis.php |