Investigating the Fate of Tetracycline in an Anaerobic Membrane Bioreactor and Its Effects on Antibiotic Resistance Gene Proliferation from Different Membrane Types

As the concern of fresh and clean water scarcity is raised worldwide, the necessity of water reuse stands out. In this sense, the AnMBR, an advanced wastewater treatment technology combining the advantages of the MBR and anaerobic processes, has gained much attention in the past years as it produces...

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Main Author: Zeeb, Reem (author)
Format: masterThesis
Published: 2022
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Online Access:http://hdl.handle.net/10725/14573
https://doi.org/10.26756/th.2022.526
http://libraries.lau.edu.lb/research/laur/terms-of-use/thesis.php
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author Zeeb, Reem
author_facet Zeeb, Reem
author_role author
dc.creator.none.fl_str_mv Zeeb, Reem
dc.date.none.fl_str_mv 2022
2022-12-20
2023-03-16T09:56:19Z
2023-03-16T09:56:19Z
dc.identifier.none.fl_str_mv http://hdl.handle.net/10725/14573
https://doi.org/10.26756/th.2022.526
http://libraries.lau.edu.lb/research/laur/terms-of-use/thesis.php
dc.language.none.fl_str_mv en
dc.publisher.none.fl_str_mv Lebanese American University
dc.rights.*.fl_str_mv info:eu-repo/semantics/openAccess
dc.subject.none.fl_str_mv Sewage -- Purification -- Anaerobic treatment
Membrane reactors
Drug resistance in microorganisms
Tetracyclines
Lebanese American University -- Dissertations
Dissertations, Academic
dc.title.none.fl_str_mv Investigating the Fate of Tetracycline in an Anaerobic Membrane Bioreactor and Its Effects on Antibiotic Resistance Gene Proliferation from Different Membrane Types
dc.type.none.fl_str_mv Thesis
info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/masterThesis
description As the concern of fresh and clean water scarcity is raised worldwide, the necessity of water reuse stands out. In this sense, the AnMBR, an advanced wastewater treatment technology combining the advantages of the MBR and anaerobic processes, has gained much attention in the past years as it produces high quality effluent from an energy efficient mechanism. Even though the removal of emerging microbial contaminants generally and antibiotics specifically from wastewater has been studied, the reduction of ARGs and tetracycline, which is one of the most used antibiotics worldwide, hasn’t been explicitly looked into using AnMBRs during treatment of real municipal wastewaters. Hence, this research, composed of a pre-tetracycline addition phase and a tetracycline addition phase, aimed to examine the effect of tetracycline continuously fed at a concentration of 300 μg/L on the performance of a lab-scale AnMBR as well as to evaluate the removal efficiency of tetracycline via the AnMBR system while looking into the attribution of each removal mechanism. It also studied the proliferation of iARGs and eARGs in the effluents of three externally connected membranes: two MFs and one UF. The results showed that a slight and quickly recoverable disruption was imposed by tetracycline on the system. Tetracycline was reduced at an efficiency > 91% for the three effluents with adsorption to sludge being the primary mechanism of removal even though notable degradation averaged to 38 % was recorded. The contribution of adsorption of tetracycline to the biofilm layer developed on each of the three membranes to removal was negligible. For ARGs, an increase in the abundance of tet-associated iARGs, with variation depending on the membrane pore size, was detected in the effluent, in opposition to tet-associated eARGs and non-tet ARGs which did not show an interpretable response to tetracycline.
eu_rights_str_mv openAccess
format masterThesis
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language_invalid_str_mv en
network_acronym_str LAURepo
network_name_str Lebanese American University repository
oai_identifier_str oai:laur.lau.edu.lb:10725/14573
publishDate 2022
publisher.none.fl_str_mv Lebanese American University
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spelling Investigating the Fate of Tetracycline in an Anaerobic Membrane Bioreactor and Its Effects on Antibiotic Resistance Gene Proliferation from Different Membrane TypesZeeb, ReemSewage -- Purification -- Anaerobic treatmentMembrane reactorsDrug resistance in microorganismsTetracyclinesLebanese American University -- DissertationsDissertations, AcademicAs the concern of fresh and clean water scarcity is raised worldwide, the necessity of water reuse stands out. In this sense, the AnMBR, an advanced wastewater treatment technology combining the advantages of the MBR and anaerobic processes, has gained much attention in the past years as it produces high quality effluent from an energy efficient mechanism. Even though the removal of emerging microbial contaminants generally and antibiotics specifically from wastewater has been studied, the reduction of ARGs and tetracycline, which is one of the most used antibiotics worldwide, hasn’t been explicitly looked into using AnMBRs during treatment of real municipal wastewaters. Hence, this research, composed of a pre-tetracycline addition phase and a tetracycline addition phase, aimed to examine the effect of tetracycline continuously fed at a concentration of 300 μg/L on the performance of a lab-scale AnMBR as well as to evaluate the removal efficiency of tetracycline via the AnMBR system while looking into the attribution of each removal mechanism. It also studied the proliferation of iARGs and eARGs in the effluents of three externally connected membranes: two MFs and one UF. The results showed that a slight and quickly recoverable disruption was imposed by tetracycline on the system. Tetracycline was reduced at an efficiency > 91% for the three effluents with adsorption to sludge being the primary mechanism of removal even though notable degradation averaged to 38 % was recorded. The contribution of adsorption of tetracycline to the biofilm layer developed on each of the three membranes to removal was negligible. For ARGs, an increase in the abundance of tet-associated iARGs, with variation depending on the membrane pore size, was detected in the effluent, in opposition to tet-associated eARGs and non-tet ARGs which did not show an interpretable response to tetracycline.1 online resource (xv, 111 leaves): ill. (some col.)Includes bibliographical references (leaves 86-106)Lebanese American University2023-03-16T09:56:19Z2023-03-16T09:56:19Z20222022-12-20Thesisinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesishttp://hdl.handle.net/10725/14573https://doi.org/10.26756/th.2022.526http://libraries.lau.edu.lb/research/laur/terms-of-use/thesis.phpeninfo:eu-repo/semantics/openAccessoai:laur.lau.edu.lb:10725/145732023-11-09T07:18:04Z
spellingShingle Investigating the Fate of Tetracycline in an Anaerobic Membrane Bioreactor and Its Effects on Antibiotic Resistance Gene Proliferation from Different Membrane Types
Zeeb, Reem
Sewage -- Purification -- Anaerobic treatment
Membrane reactors
Drug resistance in microorganisms
Tetracyclines
Lebanese American University -- Dissertations
Dissertations, Academic
status_str publishedVersion
title Investigating the Fate of Tetracycline in an Anaerobic Membrane Bioreactor and Its Effects on Antibiotic Resistance Gene Proliferation from Different Membrane Types
title_full Investigating the Fate of Tetracycline in an Anaerobic Membrane Bioreactor and Its Effects on Antibiotic Resistance Gene Proliferation from Different Membrane Types
title_fullStr Investigating the Fate of Tetracycline in an Anaerobic Membrane Bioreactor and Its Effects on Antibiotic Resistance Gene Proliferation from Different Membrane Types
title_full_unstemmed Investigating the Fate of Tetracycline in an Anaerobic Membrane Bioreactor and Its Effects on Antibiotic Resistance Gene Proliferation from Different Membrane Types
title_short Investigating the Fate of Tetracycline in an Anaerobic Membrane Bioreactor and Its Effects on Antibiotic Resistance Gene Proliferation from Different Membrane Types
title_sort Investigating the Fate of Tetracycline in an Anaerobic Membrane Bioreactor and Its Effects on Antibiotic Resistance Gene Proliferation from Different Membrane Types
topic Sewage -- Purification -- Anaerobic treatment
Membrane reactors
Drug resistance in microorganisms
Tetracyclines
Lebanese American University -- Dissertations
Dissertations, Academic
url http://hdl.handle.net/10725/14573
https://doi.org/10.26756/th.2022.526
http://libraries.lau.edu.lb/research/laur/terms-of-use/thesis.php