Preliminary evaluation of technetium-99m-labeled thymoquinone
Thymoquinone (TQ) was radiolabeled with sodium pertechnetate (Na 99m TcO4) by reduction with stannous chloride (SnCl2). The labeling efficiency of 99m Tc-TQ was determined by iTLC. Labeling conditions were optimized for pH, amount of SnCl2 required for radiolabeling and incubation time. Specifically...
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2017
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| Online Access: | http://hdl.handle.net/10725/8400 http://libraries.lau.edu.lb/research/laur/terms-of-use/articles.php https://www.researchgate.net/publication/320065449_Preliminary_Evaluation_of_Technetium-99m-Labeled_Thymoquinone_Radiotracer_Uptake_Studies_using_Gamma_Scintigraphy_Imaging_Technique |
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| _version_ | 1864513484601950208 |
|---|---|
| author | Elaridi, Jomana |
| author2 | Velho-Pereira, Reelma Gaikwad, Rajiv V. |
| author2_role | author author |
| author_facet | Elaridi, Jomana Velho-Pereira, Reelma Gaikwad, Rajiv V. |
| author_role | author |
| dc.creator.none.fl_str_mv | Elaridi, Jomana Velho-Pereira, Reelma Gaikwad, Rajiv V. |
| dc.date.none.fl_str_mv | 2017 2018-08-30T11:54:46Z 2018-08-30T11:54:46Z 2018-08-30 |
| dc.identifier.none.fl_str_mv | 0975-8585 http://hdl.handle.net/10725/8400 Velho-Pereira, R., Jagtap, A., Gaikwad, R.,& Elaridi J. (2015). Preliminary Evaluation of Technetium-99m-Labeled Thymoquinone: Radiotracer Uptake Studies using Gamma Scintigraphy Imaging Technique. Research Journal of Pharmaceutical, Biological and Chemical Sciences 8(5): 603 http://libraries.lau.edu.lb/research/laur/terms-of-use/articles.php https://www.researchgate.net/publication/320065449_Preliminary_Evaluation_of_Technetium-99m-Labeled_Thymoquinone_Radiotracer_Uptake_Studies_using_Gamma_Scintigraphy_Imaging_Technique |
| dc.language.none.fl_str_mv | en |
| dc.relation.none.fl_str_mv | Research Journal of Pharmaceutical, Biological and Chemical Sciences |
| dc.rights.*.fl_str_mv | info:eu-repo/semantics/openAccess |
| dc.title.none.fl_str_mv | Preliminary evaluation of technetium-99m-labeled thymoquinone radiotracer uptake studies using gamma scintigraphy imaging technique |
| dc.type.none.fl_str_mv | Article info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article |
| description | Thymoquinone (TQ) was radiolabeled with sodium pertechnetate (Na 99m TcO4) by reduction with stannous chloride (SnCl2). The labeling efficiency of 99m Tc-TQ was determined by iTLC. Labeling conditions were optimized for pH, amount of SnCl2 required for radiolabeling and incubation time. Specifically, direct radiolabeling of 2.5 mg/ml TQ with 250 µg/ml of stannous chloride at pH 7 with gentle mixing and incubation for 30 minutes generated the desired 99m Tc-TQ complex with 95% labeling efficiency. The in vitro stability in serum and physiologic saline showed that the radiolabeled complex was stable for 24 h. Based on the percent injected dose (%ID), the highest uptake of the 99m Tc-TQ was found in the RES post IV injection in normal mice. Oral administration of TQ led to predominant uptake of the radiolabeled complex in the stomach and the intestine. Thus, a quick, efficient and reproducible method for radiolabeling thymoquinone with the 99m Tc-radionuclide was developed and optimized. The TQ-radiotracer was evaluated for radiochemical purity, stability and tissue and organ distribution in mice. |
| eu_rights_str_mv | openAccess |
| format | article |
| id | LAURepo_906f3129d4c81e916f6cc85cfd99cd6e |
| identifier_str_mv | 0975-8585 Velho-Pereira, R., Jagtap, A., Gaikwad, R.,& Elaridi J. (2015). Preliminary Evaluation of Technetium-99m-Labeled Thymoquinone: Radiotracer Uptake Studies using Gamma Scintigraphy Imaging Technique. Research Journal of Pharmaceutical, Biological and Chemical Sciences 8(5): 603 |
| language_invalid_str_mv | en |
| network_acronym_str | LAURepo |
| network_name_str | Lebanese American University repository |
| oai_identifier_str | oai:laur.lau.edu.lb:10725/8400 |
| publishDate | 2017 |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| spelling | Preliminary evaluation of technetium-99m-labeled thymoquinoneradiotracer uptake studies using gamma scintigraphy imaging techniqueElaridi, JomanaVelho-Pereira, ReelmaGaikwad, Rajiv V.Thymoquinone (TQ) was radiolabeled with sodium pertechnetate (Na 99m TcO4) by reduction with stannous chloride (SnCl2). The labeling efficiency of 99m Tc-TQ was determined by iTLC. Labeling conditions were optimized for pH, amount of SnCl2 required for radiolabeling and incubation time. Specifically, direct radiolabeling of 2.5 mg/ml TQ with 250 µg/ml of stannous chloride at pH 7 with gentle mixing and incubation for 30 minutes generated the desired 99m Tc-TQ complex with 95% labeling efficiency. The in vitro stability in serum and physiologic saline showed that the radiolabeled complex was stable for 24 h. Based on the percent injected dose (%ID), the highest uptake of the 99m Tc-TQ was found in the RES post IV injection in normal mice. Oral administration of TQ led to predominant uptake of the radiolabeled complex in the stomach and the intestine. Thus, a quick, efficient and reproducible method for radiolabeling thymoquinone with the 99m Tc-radionuclide was developed and optimized. The TQ-radiotracer was evaluated for radiochemical purity, stability and tissue and organ distribution in mice.PublishedN/A2018-08-30T11:54:46Z2018-08-30T11:54:46Z20172018-08-30Articleinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article0975-8585http://hdl.handle.net/10725/8400Velho-Pereira, R., Jagtap, A., Gaikwad, R.,& Elaridi J. (2015). Preliminary Evaluation of Technetium-99m-Labeled Thymoquinone: Radiotracer Uptake Studies using Gamma Scintigraphy Imaging Technique. Research Journal of Pharmaceutical, Biological and Chemical Sciences 8(5): 603http://libraries.lau.edu.lb/research/laur/terms-of-use/articles.phphttps://www.researchgate.net/publication/320065449_Preliminary_Evaluation_of_Technetium-99m-Labeled_Thymoquinone_Radiotracer_Uptake_Studies_using_Gamma_Scintigraphy_Imaging_TechniqueenResearch Journal of Pharmaceutical, Biological and Chemical Sciencesinfo:eu-repo/semantics/openAccessoai:laur.lau.edu.lb:10725/84002021-03-19T10:43:17Z |
| spellingShingle | Preliminary evaluation of technetium-99m-labeled thymoquinone Elaridi, Jomana |
| status_str | publishedVersion |
| title | Preliminary evaluation of technetium-99m-labeled thymoquinone |
| title_full | Preliminary evaluation of technetium-99m-labeled thymoquinone |
| title_fullStr | Preliminary evaluation of technetium-99m-labeled thymoquinone |
| title_full_unstemmed | Preliminary evaluation of technetium-99m-labeled thymoquinone |
| title_short | Preliminary evaluation of technetium-99m-labeled thymoquinone |
| title_sort | Preliminary evaluation of technetium-99m-labeled thymoquinone |
| url | http://hdl.handle.net/10725/8400 http://libraries.lau.edu.lb/research/laur/terms-of-use/articles.php https://www.researchgate.net/publication/320065449_Preliminary_Evaluation_of_Technetium-99m-Labeled_Thymoquinone_Radiotracer_Uptake_Studies_using_Gamma_Scintigraphy_Imaging_Technique |