MicroRNA profiling in intraocular medulloepitheliomas
Purpose To study the differential expression of microRNA (miRNA) profiles between intraocular medulloepithelioma (ME) and normal control tissue (CT). Material and Methods Total RNA was extracted from formalin fixed paraffin embedded (FFPE) intraocular ME (n=7) and from age matched ciliary body contr...
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| المؤلف الرئيسي: | |
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| مؤلفون آخرون: | , , , , , |
| التنسيق: | article |
| منشور في: |
2015
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| الوصول للمادة أونلاين: | http://hdl.handle.net/10725/10828 https://doi.org/10.1371/journal.pone.0121706 http://libraries.lau.edu.lb/research/laur/terms-of-use/articles.php https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0121706 |
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| _version_ | 1864513467500724224 |
|---|---|
| author | Edward, Deepak P. |
| author2 | Alkatan, Hind Qundeel, Rafiq Eberhart, Charles Al Mesfer, Saleh Ghazi, Nicola Al Safieh, Leen |
| author2_role | author author author author author author |
| author_facet | Edward, Deepak P. Alkatan, Hind Qundeel, Rafiq Eberhart, Charles Al Mesfer, Saleh Ghazi, Nicola Al Safieh, Leen |
| author_role | author |
| dc.creator.none.fl_str_mv | Edward, Deepak P. Alkatan, Hind Qundeel, Rafiq Eberhart, Charles Al Mesfer, Saleh Ghazi, Nicola Al Safieh, Leen |
| dc.date.none.fl_str_mv | 2015 2019-06-14T11:05:05Z 2019-06-14T11:05:05Z 2019-06-14 |
| dc.identifier.none.fl_str_mv | 1932-6203 http://hdl.handle.net/10725/10828 https://doi.org/10.1371/journal.pone.0121706 Edward, D. P., Alkatan, H., Rafiq, Q., Eberhart, C., Al Mesfer, S., Ghazi, N., ... & Amero, K. K. A. (2015). MicroRNA profiling in intraocular medulloepitheliomas. PloS one, 10(3), e0121706. http://libraries.lau.edu.lb/research/laur/terms-of-use/articles.php https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0121706 |
| dc.language.none.fl_str_mv | en |
| dc.relation.none.fl_str_mv | Plos one |
| dc.rights.*.fl_str_mv | info:eu-repo/semantics/openAccess |
| dc.title.none.fl_str_mv | MicroRNA profiling in intraocular medulloepitheliomas |
| dc.type.none.fl_str_mv | Article info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article |
| description | Purpose To study the differential expression of microRNA (miRNA) profiles between intraocular medulloepithelioma (ME) and normal control tissue (CT). Material and Methods Total RNA was extracted from formalin fixed paraffin embedded (FFPE) intraocular ME (n=7) and from age matched ciliary body controls (n=8). The clinical history and phenotype was recorded. MiRNA profiles were determined using the Affymetrix GeneChip miRNA Arrays analyzed using expression console 1.3 software. Validation of significantly dysregulated miRNA was confimed by quantitaive real-time PCR. The web-based DNA Intelligent Analysis (DIANA)-miRPath v2.0 was used to perform enrichment analysis of differentially expressed (DE) miRNA gene targets in Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway. Results The pathologic evaluation revealed one benign (benign non-teratoid, n=1) and six malignant tumors (malignant teratoid, n=2; malignant non-teratoid, n = 4). A total of 88 miRNAs were upregulated and 43 miRNAs were downregulated significantly (P<0.05) in the tumor specimens. Many of these significantly dysregulated miRNAs were known to play various roles in carcinogenesis and tumor behavior. RT-PCR validated three significantly upregulated miRNAs and three significantly downregulated miRNAs namely miR-217, miR-216a, miR-216b, miR-146a, miR-509-3p and miR-211. Many DE miRNAs that were significant in ME tumors showed dysregulation in retinoblastoma, glioblastoma, and precursor, normal and reactive human cartilage. Enriched pathway analysis suggested a significant association of upregulated miRNAs with 15 pathways involved in prion disease and several types of cancer. The pathways involving significantly downregulated miRNAs included the toll-like receptor (TLR) (p<4.36E-16) and Nuclear Factor kappa B (NF-κB) signaling pathways (p<9.00E-06). Conclusions We report significantly dysregulated miRNAs in intraocular ME tumors, which exhibited abnormal profiles in other cancers as well such as retinoblastoma and glioblastoma. Pathway analysis of all dysregulated miRNAs shared commonalities with other cancer pathways. |
| eu_rights_str_mv | openAccess |
| format | article |
| id | LAURepo_92d148c9fdc582c8109abd630d09b73e |
| identifier_str_mv | 1932-6203 Edward, D. P., Alkatan, H., Rafiq, Q., Eberhart, C., Al Mesfer, S., Ghazi, N., ... & Amero, K. K. A. (2015). MicroRNA profiling in intraocular medulloepitheliomas. PloS one, 10(3), e0121706. |
| language_invalid_str_mv | en |
| network_acronym_str | LAURepo |
| network_name_str | Lebanese American University repository |
| oai_identifier_str | oai:laur.lau.edu.lb:10725/10828 |
| publishDate | 2015 |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| spelling | MicroRNA profiling in intraocular medulloepitheliomasEdward, Deepak P.Alkatan, HindQundeel, RafiqEberhart, CharlesAl Mesfer, SalehGhazi, NicolaAl Safieh, LeenPurpose To study the differential expression of microRNA (miRNA) profiles between intraocular medulloepithelioma (ME) and normal control tissue (CT). Material and Methods Total RNA was extracted from formalin fixed paraffin embedded (FFPE) intraocular ME (n=7) and from age matched ciliary body controls (n=8). The clinical history and phenotype was recorded. MiRNA profiles were determined using the Affymetrix GeneChip miRNA Arrays analyzed using expression console 1.3 software. Validation of significantly dysregulated miRNA was confimed by quantitaive real-time PCR. The web-based DNA Intelligent Analysis (DIANA)-miRPath v2.0 was used to perform enrichment analysis of differentially expressed (DE) miRNA gene targets in Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway. Results The pathologic evaluation revealed one benign (benign non-teratoid, n=1) and six malignant tumors (malignant teratoid, n=2; malignant non-teratoid, n = 4). A total of 88 miRNAs were upregulated and 43 miRNAs were downregulated significantly (P<0.05) in the tumor specimens. Many of these significantly dysregulated miRNAs were known to play various roles in carcinogenesis and tumor behavior. RT-PCR validated three significantly upregulated miRNAs and three significantly downregulated miRNAs namely miR-217, miR-216a, miR-216b, miR-146a, miR-509-3p and miR-211. Many DE miRNAs that were significant in ME tumors showed dysregulation in retinoblastoma, glioblastoma, and precursor, normal and reactive human cartilage. Enriched pathway analysis suggested a significant association of upregulated miRNAs with 15 pathways involved in prion disease and several types of cancer. The pathways involving significantly downregulated miRNAs included the toll-like receptor (TLR) (p<4.36E-16) and Nuclear Factor kappa B (NF-κB) signaling pathways (p<9.00E-06). Conclusions We report significantly dysregulated miRNAs in intraocular ME tumors, which exhibited abnormal profiles in other cancers as well such as retinoblastoma and glioblastoma. Pathway analysis of all dysregulated miRNAs shared commonalities with other cancer pathways.PublishedN/A2019-06-14T11:05:05Z2019-06-14T11:05:05Z20152019-06-14Articleinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1932-6203http://hdl.handle.net/10725/10828https://doi.org/10.1371/journal.pone.0121706Edward, D. P., Alkatan, H., Rafiq, Q., Eberhart, C., Al Mesfer, S., Ghazi, N., ... & Amero, K. K. A. (2015). MicroRNA profiling in intraocular medulloepitheliomas. PloS one, 10(3), e0121706.http://libraries.lau.edu.lb/research/laur/terms-of-use/articles.phphttps://journals.plos.org/plosone/article?id=10.1371/journal.pone.0121706enPlos oneinfo:eu-repo/semantics/openAccessoai:laur.lau.edu.lb:10725/108282021-03-19T10:45:19Z |
| spellingShingle | MicroRNA profiling in intraocular medulloepitheliomas Edward, Deepak P. |
| status_str | publishedVersion |
| title | MicroRNA profiling in intraocular medulloepitheliomas |
| title_full | MicroRNA profiling in intraocular medulloepitheliomas |
| title_fullStr | MicroRNA profiling in intraocular medulloepitheliomas |
| title_full_unstemmed | MicroRNA profiling in intraocular medulloepitheliomas |
| title_short | MicroRNA profiling in intraocular medulloepitheliomas |
| title_sort | MicroRNA profiling in intraocular medulloepitheliomas |
| url | http://hdl.handle.net/10725/10828 https://doi.org/10.1371/journal.pone.0121706 http://libraries.lau.edu.lb/research/laur/terms-of-use/articles.php https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0121706 |