11p15 DNA-methylation analysis in monozygotic twins with discordant intrauterine development due to severe twin-to-twin transfusion syndrome
Background Prenatal growth restriction and low birth weight have been linked to long-term alterations of health, presumably via adaptive modifications of the epigenome. Recent studies indicate a plasticity of the 11p15 epigenotype in response to environmental changes during early stages of human dev...
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| مؤلفون آخرون: | , , , , , , |
| التنسيق: | article |
| منشور في: |
2013
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| الوصول للمادة أونلاين: | http://hdl.handle.net/10725/6195 https://doi.org/10.1186/1868-7083-6-6 http://libraries.lau.edu.lb/research/laur/terms-of-use/articles.php https://clinicalepigeneticsjournal.biomedcentral.com/articles/10.1186/1868-7083-6-6 |
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| _version_ | 1864513478886162432 |
|---|---|
| author | El Maarri, Osman |
| author2 | Schreiner, Felix Gohlke, Bettina Stutte, Sonja Bartmann, Peter Hecher, Kurt Oldenburg, Johannes Woelfle, Joachim |
| author2_role | author author author author author author author |
| author_facet | El Maarri, Osman Schreiner, Felix Gohlke, Bettina Stutte, Sonja Bartmann, Peter Hecher, Kurt Oldenburg, Johannes Woelfle, Joachim |
| author_role | author |
| dc.creator.none.fl_str_mv | El Maarri, Osman Schreiner, Felix Gohlke, Bettina Stutte, Sonja Bartmann, Peter Hecher, Kurt Oldenburg, Johannes Woelfle, Joachim |
| dc.date.none.fl_str_mv | 2013 2017-09-14T12:49:01Z 2017-09-14T12:49:01Z 2017-09-14 |
| dc.identifier.none.fl_str_mv | 1868-7083 http://hdl.handle.net/10725/6195 https://doi.org/10.1186/1868-7083-6-6 Schreiner, F., Gohlke, B., Stutte, S., Bartmann, P., Hecher, K., Oldenburg, J., ... & Woelfle, J. (2014). 11p15 DNA-methylation analysis in monozygotic twins with discordant intrauterine development due to severe twin-to-twin transfusion syndrome. Clinical epigenetics, 6(1), 6. http://libraries.lau.edu.lb/research/laur/terms-of-use/articles.php https://clinicalepigeneticsjournal.biomedcentral.com/articles/10.1186/1868-7083-6-6 |
| dc.language.none.fl_str_mv | en |
| dc.relation.none.fl_str_mv | Clinical Epigenetics |
| dc.rights.*.fl_str_mv | info:eu-repo/semantics/openAccess |
| dc.title.none.fl_str_mv | 11p15 DNA-methylation analysis in monozygotic twins with discordant intrauterine development due to severe twin-to-twin transfusion syndrome |
| dc.type.none.fl_str_mv | Article info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article |
| description | Background Prenatal growth restriction and low birth weight have been linked to long-term alterations of health, presumably via adaptive modifications of the epigenome. Recent studies indicate a plasticity of the 11p15 epigenotype in response to environmental changes during early stages of human development. Study design We analyzed methylation levels at different 11p15 loci in 20 growth-discordant monozygotic twin pairs. Intrauterine development was discordant due to severe twin-to-twin transfusion syndrome (TTTS), which was treated by fetoscopic laser coagulation of communicating vessels before 25 weeks of gestation. Methylation levels at age 4 were determined in blood and buccal cell-derived DNA by the single nucleotide primer extension reaction ion pair reverse-phase high performance liquid chromatography (SNuPE IP RP HPLC) assay. Methylation at LINE-1 repeats was analyzed as an estimate of global methylation. Results In general, variance of locus-specific methylation levels appeared to be higher in buccal cell- as compared to blood cell-derived DNA samples. Paired analyses within the twin pairs revealed significant differences at only one CpG site (IGF2 dmr0 SN3 (blood), +1.9% in donors; P = 0.013). When plotting the twin pair-discordance in birth weight against the degree of discordance in site-specific methylation at age 4, only a few CpGs were found to interact (one CpG site each at IGF2dmr0 in blood/saliva DNA, one CpG at LINE-1 repeats in saliva DNA), with 26 to 36% of the intra-twin pair divergence at these sites explained by prenatal growth discordance. However, across the entire cohort of 40 children, site-specific methylation did not correlate with SD-scores for weight or length at birth. Insulin-like growth factor-II serum concentrations showed significant within-twin pair correlations at birth (R = 0.57) and at age 4 (R = 0.79), but did not differ between donors and recipients. They also did not correlate with the analyzed 11p15 methylation parameters. Conclusion In a cohort of 20 growth-discordant monozygotic twin pairs, severe alteration in placental blood supply due to TTTS appears to leave only weak, if any, epigenetic marks at the analyzed CpG sites at 11p15. |
| eu_rights_str_mv | openAccess |
| format | article |
| id | LAURepo_979bb630cfae38720da0801cdbf67323 |
| identifier_str_mv | 1868-7083 Schreiner, F., Gohlke, B., Stutte, S., Bartmann, P., Hecher, K., Oldenburg, J., ... & Woelfle, J. (2014). 11p15 DNA-methylation analysis in monozygotic twins with discordant intrauterine development due to severe twin-to-twin transfusion syndrome. Clinical epigenetics, 6(1), 6. |
| language_invalid_str_mv | en |
| network_acronym_str | LAURepo |
| network_name_str | Lebanese American University repository |
| oai_identifier_str | oai:laur.lau.edu.lb:10725/6195 |
| publishDate | 2013 |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| spelling | 11p15 DNA-methylation analysis in monozygotic twins with discordant intrauterine development due to severe twin-to-twin transfusion syndromeEl Maarri, OsmanSchreiner, FelixGohlke, BettinaStutte, SonjaBartmann, PeterHecher, KurtOldenburg, JohannesWoelfle, JoachimBackground Prenatal growth restriction and low birth weight have been linked to long-term alterations of health, presumably via adaptive modifications of the epigenome. Recent studies indicate a plasticity of the 11p15 epigenotype in response to environmental changes during early stages of human development. Study design We analyzed methylation levels at different 11p15 loci in 20 growth-discordant monozygotic twin pairs. Intrauterine development was discordant due to severe twin-to-twin transfusion syndrome (TTTS), which was treated by fetoscopic laser coagulation of communicating vessels before 25 weeks of gestation. Methylation levels at age 4 were determined in blood and buccal cell-derived DNA by the single nucleotide primer extension reaction ion pair reverse-phase high performance liquid chromatography (SNuPE IP RP HPLC) assay. Methylation at LINE-1 repeats was analyzed as an estimate of global methylation. Results In general, variance of locus-specific methylation levels appeared to be higher in buccal cell- as compared to blood cell-derived DNA samples. Paired analyses within the twin pairs revealed significant differences at only one CpG site (IGF2 dmr0 SN3 (blood), +1.9% in donors; P = 0.013). When plotting the twin pair-discordance in birth weight against the degree of discordance in site-specific methylation at age 4, only a few CpGs were found to interact (one CpG site each at IGF2dmr0 in blood/saliva DNA, one CpG at LINE-1 repeats in saliva DNA), with 26 to 36% of the intra-twin pair divergence at these sites explained by prenatal growth discordance. However, across the entire cohort of 40 children, site-specific methylation did not correlate with SD-scores for weight or length at birth. Insulin-like growth factor-II serum concentrations showed significant within-twin pair correlations at birth (R = 0.57) and at age 4 (R = 0.79), but did not differ between donors and recipients. They also did not correlate with the analyzed 11p15 methylation parameters. Conclusion In a cohort of 20 growth-discordant monozygotic twin pairs, severe alteration in placental blood supply due to TTTS appears to leave only weak, if any, epigenetic marks at the analyzed CpG sites at 11p15.PublishedN/A2017-09-14T12:49:01Z2017-09-14T12:49:01Z20132017-09-14Articleinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1868-7083http://hdl.handle.net/10725/6195https://doi.org/10.1186/1868-7083-6-6Schreiner, F., Gohlke, B., Stutte, S., Bartmann, P., Hecher, K., Oldenburg, J., ... & Woelfle, J. (2014). 11p15 DNA-methylation analysis in monozygotic twins with discordant intrauterine development due to severe twin-to-twin transfusion syndrome. Clinical epigenetics, 6(1), 6.http://libraries.lau.edu.lb/research/laur/terms-of-use/articles.phphttps://clinicalepigeneticsjournal.biomedcentral.com/articles/10.1186/1868-7083-6-6enClinical Epigeneticsinfo:eu-repo/semantics/openAccessoai:laur.lau.edu.lb:10725/61952021-03-19T10:43:09Z |
| spellingShingle | 11p15 DNA-methylation analysis in monozygotic twins with discordant intrauterine development due to severe twin-to-twin transfusion syndrome El Maarri, Osman |
| status_str | publishedVersion |
| title | 11p15 DNA-methylation analysis in monozygotic twins with discordant intrauterine development due to severe twin-to-twin transfusion syndrome |
| title_full | 11p15 DNA-methylation analysis in monozygotic twins with discordant intrauterine development due to severe twin-to-twin transfusion syndrome |
| title_fullStr | 11p15 DNA-methylation analysis in monozygotic twins with discordant intrauterine development due to severe twin-to-twin transfusion syndrome |
| title_full_unstemmed | 11p15 DNA-methylation analysis in monozygotic twins with discordant intrauterine development due to severe twin-to-twin transfusion syndrome |
| title_short | 11p15 DNA-methylation analysis in monozygotic twins with discordant intrauterine development due to severe twin-to-twin transfusion syndrome |
| title_sort | 11p15 DNA-methylation analysis in monozygotic twins with discordant intrauterine development due to severe twin-to-twin transfusion syndrome |
| url | http://hdl.handle.net/10725/6195 https://doi.org/10.1186/1868-7083-6-6 http://libraries.lau.edu.lb/research/laur/terms-of-use/articles.php https://clinicalepigeneticsjournal.biomedcentral.com/articles/10.1186/1868-7083-6-6 |