Hepatoprotective activity of the wild carrot chloroform-based fraction against CCl4-induced liver toxicity in mice
The liver plays a major role in detoxifying and clearing xenobiotics that may lead to liver damage and hepatic dysfunction. The present study was carried out to evaluate the hepatoprotective activities of the chloroform-based fraction of wild carrot (Daucus carota L. ssp. carota; Apiacae) on CCl4-in...
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| Other Authors: | , , , , |
| Format: | article |
| Published: |
2014
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| Online Access: | http://hdl.handle.net/10725/7425 http://dx.doi.org/10.1055/s-0034-1395009 http://libraries.lau.edu.lb/research/laur/terms-of-use/articles.php https://www.thieme-connect.com/products/ejournals/abstract/10.1055/s-0034-1395009 |
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| Summary: | The liver plays a major role in detoxifying and clearing xenobiotics that may lead to liver damage and hepatic dysfunction. The present study was carried out to evaluate the hepatoprotective activities of the chloroform-based fraction of wild carrot (Daucus carota L. ssp. carota; Apiacae) on CCl4-induced liver damage in BALB/c mice. Liver damage was induced by intraperitoneal injection of CCl4 at a dose of 1 ml/kg body weight. Animals were treated with different doses of the extract (50, 100 and 200 mg/kg body weight). Serum and liver homogenate supernatants were analyzed for marker enzymes aspartate transaminase (AST), alanine transaminase (ALT), catalase (CAT), superoxide dismutase (SOD) and glutathione S-transferase (GST) activities. Pretreated groups with chloroform-based fraction reduced significantly the serum levels of AST and ALT. It also reversed the CCl4-induced decrease in SOD, CAT and GST levels thereby reducing significantly hepatic damage. HPLC analysis revealed that the chloroform-based fraction was rich in polyphenolic compounds such as luteolin, kaempferol, apigenin and quercetin, which could be partially responsible for the hepatoprotective effect. The current results suggest that chloroform-based fraction possess strong hepatoprotective activity against CCl4 induced liver damage at all doses used |
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