Molecular, antibiogram and serological typing of Staphylococcus aureus isolates recovered from Al-Makased hospital in East Jerusalem
Staphylococcus aureus is a major cause of nosocomial infections and a risk in patients who have either undergone surgery or are on haemodialysis. The S. aureus infections in patients admitted to the clinical departments of Al-Makased Charitable Hospital in Jerusalem during a period of one year were...
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| المؤلف الرئيسي: | |
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| مؤلفون آخرون: | , , , , |
| التنسيق: | article |
| منشور في: |
2002
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| الوصول للمادة أونلاين: | http://hdl.handle.net/10725/3565 http://dx.doi.org/10.1046/j.1365-3156.1998.00265.x http://onlinelibrary.wiley.com/doi/10.1046/j.1365-3156.1998.00265.x/full |
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| _version_ | 1864513461628698624 |
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| author | Na'was, Tarek |
| author2 | Essawi, Tamer Hawwari, Abbass Wadi, Suzan Doudin, Anwar Fattom, Ali Ibrahim |
| author2_role | author author author author author |
| author_facet | Na'was, Tarek Essawi, Tamer Hawwari, Abbass Wadi, Suzan Doudin, Anwar Fattom, Ali Ibrahim |
| author_role | author |
| dc.creator.none.fl_str_mv | Na'was, Tarek Essawi, Tamer Hawwari, Abbass Wadi, Suzan Doudin, Anwar Fattom, Ali Ibrahim |
| dc.date.none.fl_str_mv | 2002 2016-04-14T13:49:20Z 2016-04-14T13:49:20Z 2016-04-14 |
| dc.identifier.none.fl_str_mv | 1360-2276 http://hdl.handle.net/10725/3565 http://dx.doi.org/10.1046/j.1365-3156.1998.00265.x Essawi, T., Na'was, T., Hawwari, A., Wadi, S., Doudin, A., & Fattom, A. I. (1998). Molecular, antibiogram and serological typing of Staphylococcus aureus isolates recovered from Al‐Makased hospital in East Jerusalem. Tropical Medicine & International Health, 3(7), 576-583. http://onlinelibrary.wiley.com/doi/10.1046/j.1365-3156.1998.00265.x/full |
| dc.language.none.fl_str_mv | en |
| dc.relation.none.fl_str_mv | Tropical Medicine & International Health |
| dc.rights.*.fl_str_mv | info:eu-repo/semantics/openAccess |
| dc.title.none.fl_str_mv | Molecular, antibiogram and serological typing of Staphylococcus aureus isolates recovered from Al-Makased hospital in East Jerusalem |
| dc.type.none.fl_str_mv | Article info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article |
| description | Staphylococcus aureus is a major cause of nosocomial infections and a risk in patients who have either undergone surgery or are on haemodialysis. The S. aureus infections in patients admitted to the clinical departments of Al-Makased Charitable Hospital in Jerusalem during a period of one year were investigated. Isolates included were from blood, surgical wounds, or other nonsuperficial sites. Of 63 isolates available for analysis, 46 (73.0%) expressed type 8 capsular polysaccharide; 13 (20.7%), type 5 capsular polysaccharide; only 4 isolates (6.3%) did not express type 5 or type 8 antibodies. The strains fitted in 7 different antibiogram types, with the type showing resistance only to penicillin and ampicillin prevalent in 34 out of 63 isolates (54.0%). Of the 12 methicillin-resistant S. aureus (MRSA) isolates (19.1%), 8(66.7%) possessed the type 8 capsule and 4(33.7%) the type 5 capsule. Pulsed-field gel electrophoresis of all isolates with the restriction-endonuclease enzymes Sma I revealed 34 patterns demonstrating that no single methicillin-sensitive S. aureus strain was endemic in the hospital. However, all MRSA isolates with a type 8 capsule showed identical PFGE patterns using the 2 restriction-endonuclease enzymes Sma I and SST II. Moreover, type 5 isolates showed identical patterns (one isolate differed from the rest with one band only). These data suggest and confirm the clonality of type 5 and type 8 MRSA isolates. Analysing the results of the capsular and antibiogram typing schemes in conjunction proved useful and suggested that such an analysis can be employed as a helpful epidemiological tool in hospitals with limited resources. |
| eu_rights_str_mv | openAccess |
| format | article |
| id | LAURepo_b4911ece5d225d814e2b1acef23a382d |
| identifier_str_mv | 1360-2276 Essawi, T., Na'was, T., Hawwari, A., Wadi, S., Doudin, A., & Fattom, A. I. (1998). Molecular, antibiogram and serological typing of Staphylococcus aureus isolates recovered from Al‐Makased hospital in East Jerusalem. Tropical Medicine & International Health, 3(7), 576-583. |
| language_invalid_str_mv | en |
| network_acronym_str | LAURepo |
| network_name_str | Lebanese American University repository |
| oai_identifier_str | oai:laur.lau.edu.lb:10725/3565 |
| publishDate | 2002 |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| spelling | Molecular, antibiogram and serological typing of Staphylococcus aureus isolates recovered from Al-Makased hospital in East JerusalemNa'was, TarekEssawi, TamerHawwari, AbbassWadi, SuzanDoudin, AnwarFattom, Ali IbrahimStaphylococcus aureus is a major cause of nosocomial infections and a risk in patients who have either undergone surgery or are on haemodialysis. The S. aureus infections in patients admitted to the clinical departments of Al-Makased Charitable Hospital in Jerusalem during a period of one year were investigated. Isolates included were from blood, surgical wounds, or other nonsuperficial sites. Of 63 isolates available for analysis, 46 (73.0%) expressed type 8 capsular polysaccharide; 13 (20.7%), type 5 capsular polysaccharide; only 4 isolates (6.3%) did not express type 5 or type 8 antibodies. The strains fitted in 7 different antibiogram types, with the type showing resistance only to penicillin and ampicillin prevalent in 34 out of 63 isolates (54.0%). Of the 12 methicillin-resistant S. aureus (MRSA) isolates (19.1%), 8(66.7%) possessed the type 8 capsule and 4(33.7%) the type 5 capsule. Pulsed-field gel electrophoresis of all isolates with the restriction-endonuclease enzymes Sma I revealed 34 patterns demonstrating that no single methicillin-sensitive S. aureus strain was endemic in the hospital. However, all MRSA isolates with a type 8 capsule showed identical PFGE patterns using the 2 restriction-endonuclease enzymes Sma I and SST II. Moreover, type 5 isolates showed identical patterns (one isolate differed from the rest with one band only). These data suggest and confirm the clonality of type 5 and type 8 MRSA isolates. Analysing the results of the capsular and antibiogram typing schemes in conjunction proved useful and suggested that such an analysis can be employed as a helpful epidemiological tool in hospitals with limited resources.PublishedN/A2016-04-14T13:49:20Z2016-04-14T13:49:20Z20022016-04-14Articleinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1360-2276http://hdl.handle.net/10725/3565http://dx.doi.org/10.1046/j.1365-3156.1998.00265.xEssawi, T., Na'was, T., Hawwari, A., Wadi, S., Doudin, A., & Fattom, A. I. (1998). Molecular, antibiogram and serological typing of Staphylococcus aureus isolates recovered from Al‐Makased hospital in East Jerusalem. Tropical Medicine & International Health, 3(7), 576-583.http://onlinelibrary.wiley.com/doi/10.1046/j.1365-3156.1998.00265.x/fullenTropical Medicine & International Healthinfo:eu-repo/semantics/openAccessoai:laur.lau.edu.lb:10725/35652021-03-19T10:00:46Z |
| spellingShingle | Molecular, antibiogram and serological typing of Staphylococcus aureus isolates recovered from Al-Makased hospital in East Jerusalem Na'was, Tarek |
| status_str | publishedVersion |
| title | Molecular, antibiogram and serological typing of Staphylococcus aureus isolates recovered from Al-Makased hospital in East Jerusalem |
| title_full | Molecular, antibiogram and serological typing of Staphylococcus aureus isolates recovered from Al-Makased hospital in East Jerusalem |
| title_fullStr | Molecular, antibiogram and serological typing of Staphylococcus aureus isolates recovered from Al-Makased hospital in East Jerusalem |
| title_full_unstemmed | Molecular, antibiogram and serological typing of Staphylococcus aureus isolates recovered from Al-Makased hospital in East Jerusalem |
| title_short | Molecular, antibiogram and serological typing of Staphylococcus aureus isolates recovered from Al-Makased hospital in East Jerusalem |
| title_sort | Molecular, antibiogram and serological typing of Staphylococcus aureus isolates recovered from Al-Makased hospital in East Jerusalem |
| url | http://hdl.handle.net/10725/3565 http://dx.doi.org/10.1046/j.1365-3156.1998.00265.x http://onlinelibrary.wiley.com/doi/10.1046/j.1365-3156.1998.00265.x/full |