WASP family members and formin proteins coordinate regulation of cell protrusions in carcinoma cells
We examined the role of the actin nucleation promoters neural Wiskott-Aldrich syndrome protein (N-WASP) and WAVE2 in cell protrusion in response to epidermal growth factor (EGF), a key regulator in carcinoma cell invasion. We found that WAVE2 knockdown (KD) suppresses lamellipod formation and increa...
Saved in:
| Main Author: | |
|---|---|
| Other Authors: | , , , , , , , , , , |
| Format: | article |
| Published: |
2008
|
| Online Access: | http://hdl.handle.net/10725/5198 http://dx.doi.org/110.1083/jcb.200708123 http://libraries.lau.edu.lb/research/laur/terms-of-use/articles.php http://jcb.rupress.org/content/180/6/1245/tab-pdf |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1864513465375260672 |
|---|---|
| author | Sarmiento, Corina |
| author2 | Wang, Weigang Dovas, Athanassios Yamaguchi, Hideki Sidani, Mazen El-Sibai, Mirvat Desmarais, Vera Holman, Holly A. Kitchen, Susan Backer, Jonathan M. Alberts, Art Condeelis, John |
| author2_role | author author author author author author author author author author author |
| author_facet | Sarmiento, Corina Wang, Weigang Dovas, Athanassios Yamaguchi, Hideki Sidani, Mazen El-Sibai, Mirvat Desmarais, Vera Holman, Holly A. Kitchen, Susan Backer, Jonathan M. Alberts, Art Condeelis, John |
| author_role | author |
| dc.creator.none.fl_str_mv | Sarmiento, Corina Wang, Weigang Dovas, Athanassios Yamaguchi, Hideki Sidani, Mazen El-Sibai, Mirvat Desmarais, Vera Holman, Holly A. Kitchen, Susan Backer, Jonathan M. Alberts, Art Condeelis, John |
| dc.date.none.fl_str_mv | 2008 2017-02-08T13:02:09Z 2017-02-08T13:02:09Z 2017-02-08 |
| dc.identifier.none.fl_str_mv | 0021-9525 http://hdl.handle.net/10725/5198 http://dx.doi.org/110.1083/jcb.200708123 Sarmiento, C., Wang, W., Dovas, A., Yamaguchi, H., Sidani, M., El-Sibai, M., ... & Alberts, A. (2008). WASP family members and formin proteins coordinate regulation of cell protrusions in carcinoma cells. The Journal of cell biology, 180(6), 1245-1260. http://libraries.lau.edu.lb/research/laur/terms-of-use/articles.php http://jcb.rupress.org/content/180/6/1245/tab-pdf |
| dc.language.none.fl_str_mv | en |
| dc.relation.none.fl_str_mv | Journal of Cell Biology |
| dc.rights.*.fl_str_mv | info:eu-repo/semantics/openAccess |
| dc.title.none.fl_str_mv | WASP family members and formin proteins coordinate regulation of cell protrusions in carcinoma cells |
| dc.type.none.fl_str_mv | Article info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article |
| description | We examined the role of the actin nucleation promoters neural Wiskott-Aldrich syndrome protein (N-WASP) and WAVE2 in cell protrusion in response to epidermal growth factor (EGF), a key regulator in carcinoma cell invasion. We found that WAVE2 knockdown (KD) suppresses lamellipod formation and increases filopod formation, whereas N-WASP KD has no effect. However, simultaneous KD of both proteins results in the formation of large jagged protrusions with lamellar properties and increased filopod formation. This suggests that another actin nucleation activity is at work in carcinoma cells in response to EGF. A mammalian Diaphanous–related formin, mDia1, localizes at the jagged protrusions in double KD cells. Constitutively active mDia1 recapitulated the phenotype, whereas inhibition of mDia1 blocked the formation of these protrusions. Increased RhoA activity, which stimulates mDia1 nucleation, was observed in the N-WASP/WAVE2 KD cells and was shown to be required for the N-WASP/WAVE2 KD phenotype. These data show that coordinate regulation between the WASP family and mDia proteins controls the balance between lamellar and lamellipodial protrusion activity. |
| eu_rights_str_mv | openAccess |
| format | article |
| id | LAURepo_b5e5965265d7765decd67ddd1800c5fb |
| identifier_str_mv | 0021-9525 Sarmiento, C., Wang, W., Dovas, A., Yamaguchi, H., Sidani, M., El-Sibai, M., ... & Alberts, A. (2008). WASP family members and formin proteins coordinate regulation of cell protrusions in carcinoma cells. The Journal of cell biology, 180(6), 1245-1260. |
| language_invalid_str_mv | en |
| network_acronym_str | LAURepo |
| network_name_str | Lebanese American University repository |
| oai_identifier_str | oai:laur.lau.edu.lb:10725/5198 |
| publishDate | 2008 |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| spelling | WASP family members and formin proteins coordinate regulation of cell protrusions in carcinoma cellsSarmiento, CorinaWang, WeigangDovas, AthanassiosYamaguchi, HidekiSidani, MazenEl-Sibai, MirvatDesmarais, VeraHolman, Holly A.Kitchen, SusanBacker, Jonathan M.Alberts, ArtCondeelis, JohnWe examined the role of the actin nucleation promoters neural Wiskott-Aldrich syndrome protein (N-WASP) and WAVE2 in cell protrusion in response to epidermal growth factor (EGF), a key regulator in carcinoma cell invasion. We found that WAVE2 knockdown (KD) suppresses lamellipod formation and increases filopod formation, whereas N-WASP KD has no effect. However, simultaneous KD of both proteins results in the formation of large jagged protrusions with lamellar properties and increased filopod formation. This suggests that another actin nucleation activity is at work in carcinoma cells in response to EGF. A mammalian Diaphanous–related formin, mDia1, localizes at the jagged protrusions in double KD cells. Constitutively active mDia1 recapitulated the phenotype, whereas inhibition of mDia1 blocked the formation of these protrusions. Increased RhoA activity, which stimulates mDia1 nucleation, was observed in the N-WASP/WAVE2 KD cells and was shown to be required for the N-WASP/WAVE2 KD phenotype. These data show that coordinate regulation between the WASP family and mDia proteins controls the balance between lamellar and lamellipodial protrusion activity.PublishedN/A2017-02-08T13:02:09Z2017-02-08T13:02:09Z20082017-02-08Articleinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article0021-9525http://hdl.handle.net/10725/5198http://dx.doi.org/110.1083/jcb.200708123Sarmiento, C., Wang, W., Dovas, A., Yamaguchi, H., Sidani, M., El-Sibai, M., ... & Alberts, A. (2008). WASP family members and formin proteins coordinate regulation of cell protrusions in carcinoma cells. The Journal of cell biology, 180(6), 1245-1260.http://libraries.lau.edu.lb/research/laur/terms-of-use/articles.phphttp://jcb.rupress.org/content/180/6/1245/tab-pdfenJournal of Cell Biologyinfo:eu-repo/semantics/openAccessoai:laur.lau.edu.lb:10725/51982021-03-19T10:03:22Z |
| spellingShingle | WASP family members and formin proteins coordinate regulation of cell protrusions in carcinoma cells Sarmiento, Corina |
| status_str | publishedVersion |
| title | WASP family members and formin proteins coordinate regulation of cell protrusions in carcinoma cells |
| title_full | WASP family members and formin proteins coordinate regulation of cell protrusions in carcinoma cells |
| title_fullStr | WASP family members and formin proteins coordinate regulation of cell protrusions in carcinoma cells |
| title_full_unstemmed | WASP family members and formin proteins coordinate regulation of cell protrusions in carcinoma cells |
| title_short | WASP family members and formin proteins coordinate regulation of cell protrusions in carcinoma cells |
| title_sort | WASP family members and formin proteins coordinate regulation of cell protrusions in carcinoma cells |
| url | http://hdl.handle.net/10725/5198 http://dx.doi.org/110.1083/jcb.200708123 http://libraries.lau.edu.lb/research/laur/terms-of-use/articles.php http://jcb.rupress.org/content/180/6/1245/tab-pdf |