Whole Genome Molecular Characterization of Methicillin Resistant Staphylococcus aureus Isolated From Clinical Settings in Lebanon
The high prevalence of multi-drug resistant Staphylococcus aureus, particularly the Methicillin-Resistant S. aureus (MRSA), has become a worldwide health concern. In this study, we used whole-genome sequencing, multi-locus Sequence Typing (MLST), S. aureus protein A (spa), and Staphylococcal Chromos...
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| Format: | masterThesis |
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2022
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| Online Access: | http://hdl.handle.net/10725/13949 https://doi.org/10.26756/th.2022.431 http://libraries.lau.edu.lb/research/laur/terms-of-use/thesis.php |
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| _version_ | 1864513468706586624 |
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| author | Almachtoub, Tia |
| author_facet | Almachtoub, Tia |
| author_role | author |
| dc.creator.none.fl_str_mv | Almachtoub, Tia |
| dc.date.none.fl_str_mv | 2022-08-18T08:11:49Z 2022-08-18T08:11:49Z 2022 2022-05-16 |
| dc.identifier.none.fl_str_mv | http://hdl.handle.net/10725/13949 https://doi.org/10.26756/th.2022.431 http://libraries.lau.edu.lb/research/laur/terms-of-use/thesis.php |
| dc.language.none.fl_str_mv | en |
| dc.publisher.none.fl_str_mv | Lebanese American University |
| dc.rights.*.fl_str_mv | info:eu-repo/semantics/openAccess |
| dc.subject.none.fl_str_mv | Staphylococcus aureus infections -- Lebanon Methicillin resistance -- Lebanon Lebanese American University -- Dissertations Dissertations, Academic |
| dc.title.none.fl_str_mv | Whole Genome Molecular Characterization of Methicillin Resistant Staphylococcus aureus Isolated From Clinical Settings in Lebanon |
| dc.type.none.fl_str_mv | Thesis info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/masterThesis |
| description | The high prevalence of multi-drug resistant Staphylococcus aureus, particularly the Methicillin-Resistant S. aureus (MRSA), has become a worldwide health concern. In this study, we used whole-genome sequencing, multi-locus Sequence Typing (MLST), S. aureus protein A (spa), and Staphylococcal Chromosomal Cassette (SCCmec) typing of MRSA and pan-genome analysis to study the molecular characteristics, resistance patterns, and relatedness of the isolates. In silico typing was used to determine the antimicrobial resistance and virulence determinants, rep families, and prophages. All the studied isolates carried the blaZ gene, and most were multi-drug resistant. Two MRSA isolates recovered from hospitalized patients were additionally resistant to vancomycin. Seven distinct MLST allelic profiles were identified, and the most common STs and spa types, respectively, were ST789 and ST1and t091 and t127. SCCmec typing of MRSA revealed the prevalence of type V(5C2). MRSA ST789-t091-V was the predominant type, but we also detected SCCmec cassette harboring ccrA1B1 and mecC2, classified as SCCmec type IX(1C2). |
| eu_rights_str_mv | openAccess |
| format | masterThesis |
| id | LAURepo_b8fac8ddeb80bc803d1c05e39cafa715 |
| language_invalid_str_mv | en |
| network_acronym_str | LAURepo |
| network_name_str | Lebanese American University repository |
| oai_identifier_str | oai:laur.lau.edu.lb:10725/13949 |
| publishDate | 2022 |
| publisher.none.fl_str_mv | Lebanese American University |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| spelling | Whole Genome Molecular Characterization of Methicillin Resistant Staphylococcus aureus Isolated From Clinical Settings in LebanonAlmachtoub, TiaStaphylococcus aureus infections -- LebanonMethicillin resistance -- LebanonLebanese American University -- DissertationsDissertations, AcademicThe high prevalence of multi-drug resistant Staphylococcus aureus, particularly the Methicillin-Resistant S. aureus (MRSA), has become a worldwide health concern. In this study, we used whole-genome sequencing, multi-locus Sequence Typing (MLST), S. aureus protein A (spa), and Staphylococcal Chromosomal Cassette (SCCmec) typing of MRSA and pan-genome analysis to study the molecular characteristics, resistance patterns, and relatedness of the isolates. In silico typing was used to determine the antimicrobial resistance and virulence determinants, rep families, and prophages. All the studied isolates carried the blaZ gene, and most were multi-drug resistant. Two MRSA isolates recovered from hospitalized patients were additionally resistant to vancomycin. Seven distinct MLST allelic profiles were identified, and the most common STs and spa types, respectively, were ST789 and ST1and t091 and t127. SCCmec typing of MRSA revealed the prevalence of type V(5C2). MRSA ST789-t091-V was the predominant type, but we also detected SCCmec cassette harboring ccrA1B1 and mecC2, classified as SCCmec type IX(1C2).1 online resource (xiii, 70 leaves): col. ill.Includes bibliographical references (leaf 53-70).Lebanese American University2022-08-18T08:11:49Z2022-08-18T08:11:49Z20222022-05-16Thesisinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesishttp://hdl.handle.net/10725/13949https://doi.org/10.26756/th.2022.431http://libraries.lau.edu.lb/research/laur/terms-of-use/thesis.phpeninfo:eu-repo/semantics/openAccessoai:laur.lau.edu.lb:10725/139492022-08-23T06:08:05Z |
| spellingShingle | Whole Genome Molecular Characterization of Methicillin Resistant Staphylococcus aureus Isolated From Clinical Settings in Lebanon Almachtoub, Tia Staphylococcus aureus infections -- Lebanon Methicillin resistance -- Lebanon Lebanese American University -- Dissertations Dissertations, Academic |
| status_str | publishedVersion |
| title | Whole Genome Molecular Characterization of Methicillin Resistant Staphylococcus aureus Isolated From Clinical Settings in Lebanon |
| title_full | Whole Genome Molecular Characterization of Methicillin Resistant Staphylococcus aureus Isolated From Clinical Settings in Lebanon |
| title_fullStr | Whole Genome Molecular Characterization of Methicillin Resistant Staphylococcus aureus Isolated From Clinical Settings in Lebanon |
| title_full_unstemmed | Whole Genome Molecular Characterization of Methicillin Resistant Staphylococcus aureus Isolated From Clinical Settings in Lebanon |
| title_short | Whole Genome Molecular Characterization of Methicillin Resistant Staphylococcus aureus Isolated From Clinical Settings in Lebanon |
| title_sort | Whole Genome Molecular Characterization of Methicillin Resistant Staphylococcus aureus Isolated From Clinical Settings in Lebanon |
| topic | Staphylococcus aureus infections -- Lebanon Methicillin resistance -- Lebanon Lebanese American University -- Dissertations Dissertations, Academic |
| url | http://hdl.handle.net/10725/13949 https://doi.org/10.26756/th.2022.431 http://libraries.lau.edu.lb/research/laur/terms-of-use/thesis.php |