IL-1 stimulates ceramide accumulation without inducing apoptosis in intestinal epithelial cells
Background: In inflammatory bowel disease (IBD), cytokine levels (such as interleukin-1 (IL-1)) are elevated. We have shown previously that IL-1 activates phospholipid signaling pathways in intestinal epithelial cells (IEC), leading to increased ceramide levels. Aim: To determine whether ceramide in...
محفوظ في:
| المؤلف الرئيسي: | |
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| مؤلفون آخرون: | , , , |
| التنسيق: | article |
| منشور في: |
2002
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| الوصول للمادة أونلاين: | http://hdl.handle.net/10725/2820 http://dx.doi.org/10.1080/09629350210313 http://www.hindawi.com/journals/mi/2002/467015/abs/ |
| الوسوم: |
إضافة وسم
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| _version_ | 1864513459429834752 |
|---|---|
| author | Homaidan, Fadia R. |
| author2 | El-Sabban, Marwan E. Chakroun, Iman El-Sibai, Mirvat Dbaibo, Ghassan S. |
| author2_role | author author author author |
| author_facet | Homaidan, Fadia R. El-Sabban, Marwan E. Chakroun, Iman El-Sibai, Mirvat Dbaibo, Ghassan S. |
| author_role | author |
| dc.creator.none.fl_str_mv | Homaidan, Fadia R. El-Sabban, Marwan E. Chakroun, Iman El-Sibai, Mirvat Dbaibo, Ghassan S. |
| dc.date.none.fl_str_mv | 2002 2015-12-17T11:29:57Z 2015-12-17T11:29:57Z 2016-04-11 |
| dc.identifier.none.fl_str_mv | 0962-9351 http://hdl.handle.net/10725/2820 http://dx.doi.org/10.1080/09629350210313 Homaidan, F. R., El-Sabban, M. E., Chakroun, I., El-Sibai, M., & Dbaibo, G. S. (2002). IL-1 stimulates ceramide accumulation without inducing apoptosis in intestinal epithelial cells. Mediators of inflammation, 11(1), 39-45. http://www.hindawi.com/journals/mi/2002/467015/abs/ |
| dc.language.none.fl_str_mv | en |
| dc.relation.none.fl_str_mv | Mediators of Inflammation |
| dc.rights.*.fl_str_mv | info:eu-repo/semantics/openAccess |
| dc.title.none.fl_str_mv | IL-1 stimulates ceramide accumulation without inducing apoptosis in intestinal epithelial cells |
| dc.type.none.fl_str_mv | Article info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article |
| description | Background: In inflammatory bowel disease (IBD), cytokine levels (such as interleukin-1 (IL-1)) are elevated. We have shown previously that IL-1 activates phospholipid signaling pathways in intestinal epithelial cells (IEC), leading to increased ceramide levels. Aim: To determine whether ceramide induces apoptosis in IEC. Methods: Apoptosis was evaluated by annexin-Vbinding or Hoechst nuclear staining. Levels of bcl-2, bcl-x, bax, p53 and p21 were determined by Western blotting, and cell cycle analysis was determined by flow cytometry. Results: IL-1 increased ceramide accumulation in a time-dependent and concentration-dependent manner with a peak response at 4 h, with [IL-1] = 30 ng/ml. Neither IL-1 nor ceramide induced apoptosis in IEC, but they increased bcl-2 levels and decreased bax and p21 levels without affecting bcl-x and p53 levels. They also caused a slight but significant increase in the G2/M phase. These data suggest a role for ceramide in IBD and suggest a possible mechanism for the enhanced tumorigenic activity in IBD patients. |
| eu_rights_str_mv | openAccess |
| format | article |
| id | LAURepo_b9e17d27401a80397d6e8d8910ffdea7 |
| identifier_str_mv | 0962-9351 Homaidan, F. R., El-Sabban, M. E., Chakroun, I., El-Sibai, M., & Dbaibo, G. S. (2002). IL-1 stimulates ceramide accumulation without inducing apoptosis in intestinal epithelial cells. Mediators of inflammation, 11(1), 39-45. |
| language_invalid_str_mv | en |
| network_acronym_str | LAURepo |
| network_name_str | Lebanese American University repository |
| oai_identifier_str | oai:laur.lau.edu.lb:10725/2820 |
| publishDate | 2002 |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| spelling | IL-1 stimulates ceramide accumulation without inducing apoptosis in intestinal epithelial cellsHomaidan, Fadia R.El-Sabban, Marwan E.Chakroun, ImanEl-Sibai, MirvatDbaibo, Ghassan S.Background: In inflammatory bowel disease (IBD), cytokine levels (such as interleukin-1 (IL-1)) are elevated. We have shown previously that IL-1 activates phospholipid signaling pathways in intestinal epithelial cells (IEC), leading to increased ceramide levels. Aim: To determine whether ceramide induces apoptosis in IEC. Methods: Apoptosis was evaluated by annexin-Vbinding or Hoechst nuclear staining. Levels of bcl-2, bcl-x, bax, p53 and p21 were determined by Western blotting, and cell cycle analysis was determined by flow cytometry. Results: IL-1 increased ceramide accumulation in a time-dependent and concentration-dependent manner with a peak response at 4 h, with [IL-1] = 30 ng/ml. Neither IL-1 nor ceramide induced apoptosis in IEC, but they increased bcl-2 levels and decreased bax and p21 levels without affecting bcl-x and p53 levels. They also caused a slight but significant increase in the G2/M phase. These data suggest a role for ceramide in IBD and suggest a possible mechanism for the enhanced tumorigenic activity in IBD patients.PublishedN/A2015-12-17T11:29:57Z2015-12-17T11:29:57Z20022016-04-11Articleinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article0962-9351http://hdl.handle.net/10725/2820http://dx.doi.org/10.1080/09629350210313Homaidan, F. R., El-Sabban, M. E., Chakroun, I., El-Sibai, M., & Dbaibo, G. S. (2002). IL-1 stimulates ceramide accumulation without inducing apoptosis in intestinal epithelial cells. Mediators of inflammation, 11(1), 39-45.http://www.hindawi.com/journals/mi/2002/467015/abs/enMediators of Inflammationinfo:eu-repo/semantics/openAccessoai:laur.lau.edu.lb:10725/28202021-03-19T09:59:49Z |
| spellingShingle | IL-1 stimulates ceramide accumulation without inducing apoptosis in intestinal epithelial cells Homaidan, Fadia R. |
| status_str | publishedVersion |
| title | IL-1 stimulates ceramide accumulation without inducing apoptosis in intestinal epithelial cells |
| title_full | IL-1 stimulates ceramide accumulation without inducing apoptosis in intestinal epithelial cells |
| title_fullStr | IL-1 stimulates ceramide accumulation without inducing apoptosis in intestinal epithelial cells |
| title_full_unstemmed | IL-1 stimulates ceramide accumulation without inducing apoptosis in intestinal epithelial cells |
| title_short | IL-1 stimulates ceramide accumulation without inducing apoptosis in intestinal epithelial cells |
| title_sort | IL-1 stimulates ceramide accumulation without inducing apoptosis in intestinal epithelial cells |
| url | http://hdl.handle.net/10725/2820 http://dx.doi.org/10.1080/09629350210313 http://www.hindawi.com/journals/mi/2002/467015/abs/ |